Quantitative Microscopy in Regeneration

Objectives

Long term goals:

Comprehensive quantitative assessment of lung micro-structure in animal models of human lung disease using a correlative approach from non-destructive imaging techniques to high-resolution electron microscopy.

Milestones: 

  • Combination of imaging techniques with lung function assays in healthy and diseased animals.
  • Application of these techniques to animal models of heart and lung disease and regeneration.
  • Further development and application of non-destructive correlative imaging techniques combined with stereology for quantitative lung imaging in animals and human biopsies (including optimized specimen preparation).
  • Establishing quantitative volume-EM of the lung (based on serial block face SEM, focussed ion beam SEM, electron tomography).

Research Focus

As part of the imaging platform in REBIRTH 2, we provide two advanced microscopy techniques: 1.) biomedical electron microscopy (EM) combined with 2.) unbiased methods for quantitative assessment of the microstructure of cells, tissues, and organs (stereology). In addition to established methods, we are involved in the further development of stereological methods and 3D imaging techniques and their application to REBIRTH target organs such as the lung. These approaches allow for high-resolution structural analysis, localization and distribution of target molecules and quantitative phenotype analysis of experimental animal models or human biopsies. Beyond providing these techniques as a service, our own research projects are focussed on the (ultra)structural analysis of the lung and its surfactant system in health and disease (e.g. animal models of ischemia/reperfusion injury, emphysema and fibrosis), as well as remodeling and regeneration of the heart.

Collaborations

  • H. Clark/J. Madsen (Southampton): Stereological analysis of the effects of irradiation and bone marrow transplantation on the mouse lung
  • A. Günther (Gießen): Surfactant in animal models of lung fibrosis
  • S. Sedej/T. Eisenberg/F. Madeo (Graz): Effect of spermidine on aging-associated changes of the heart
  • M. Beers (Philadelphia): Lung phenotype analysis of mouse models of lung disease (emphysema, fibrosis) based on surfactant alterations
  • I. Gruh, U. Martin, REBIRTH Unit Myocardial Tissue Engineering: EM characterization of stem cell derived target cell differentiation (lung, heart)
  • A. Heisterkamp, T. Ripken, H. Meyer, Laser Manipulation and Cellular Engineering, Laser Zentrum Hannover (): Scanning laser optical tomography (SLOT)
  • T. Wahlers, T. Wittwer (Cologne): Stem-cell based lung preservation in experimental lung transplantation
  • T. Geiser (Bern): Resident pulmonary stem cells in lung fibrosis

Publications

2013 - ongoing

2017

Schnapper A, Christmann A, Knudsen L, Rahmanian P, Choi YH, Zeriouh M, Karavidic S, Neef K, Sterner-Kock A, Guschlbauer M, Hofmaier F, Maul AC, Wittwer T, Wahlers T, Muhlfeld C, Ochs M. Stereological assessment of the blood-air barrier and the surfactant system after mesenchymal stem cell pretreatment in a porcine non-heart-beating donor model for lung transplantation. J Anat. 2017/12/02 ed2017.

Piatkowski T, Brandenberger C, Rahmanian P, Choi YH, Zeriouh M, Sabashnikov A, Wittwer T, Wahlers TCW, Ochs M, Muhlfeld C. Localization of Exogenous Mesenchymal Stem Cells in a Pig Model of Lung Transplantation. Thorac Cardiovasc Surg. 2017/10/11 ed2017.

Jacob A, Morley M, Hawkins F, McCauley KB, Jean JC, Heins H, Na CL, Weaver TE, Vedaie M, Hurley K, Hinds A, Russo SJ, Kook S, Zacharias W, Ochs M, Traber K, Quinton LJ, Crane A, Davis BR, White FV, Wambach J, Whitsett JA, Cole FS, Morrisey EE, Guttentag SH, Beers MF, Kotton DN. Differentiation of Human Pluripotent Stem Cells into Functional Lung Alveolar Epithelial Cells. Cell Stem Cell. 2017/10/03 ed2017. p. 472-88 e10.

Opitz L, Kling KM, Brandenberger C, Muhlfeld C. Lipid-body containing interstitial cells (lipofibroblasts) in the lungs of various mouse strains. J Anat. 2017/08/09 ed2017. p. 970-7.

Schipke J, Gonzalez-Tendero A, Cornejo L, Willfuhr A, Bijnens B, Crispi F, Muhlfeld C, Gratacos E. Experimentally induced intrauterine growth restriction in rabbits leads to differential remodelling of left versus right ventricular myocardial microstructure. Histochem Cell Biol. 2017/07/12 ed2017. p. 557-67.

Stauber M, Boldt K, Wrede C, Weidemann M, Kellner M, Schuster-Gossler K, Kuhnel MP, Hegermann J, Ueffing M, Gossler A. 1700012B09Rik, a FOXJ1 effector gene active in ciliated tissues of the mouse but not essential for motile ciliogenesis. Dev Biol. 2017/07/02 ed2017. p. 186-99.

Neves J, Leitz D, Kraut S, Brandenberger C, Agrawal R, Weissmann N, Muhlfeld C, Mall MA, Altamura S, Muckenthaler MU. Disruption of the Hepcidin/Ferroportin Regulatory System Causes Pulmonary Iron Overload and Restrictive Lung Disease. EBioMedicine. 2017/05/14 ed2017. p. 230-9.

Bhayana S, Baisantry A, Kraemer TD, Wrede C, Hegermann J, Brasen JH, Bockmeyer C, Ulrich Becker J, Ochs M, Gwinner W, Haller H, Melk A, Schmitt R. Autophagy in kidney transplants of sirolimus treated recipients. J Nephropathol. 2017/05/12 ed2017. p. 90-6.

Granados M, Morticelli L, Andriopoulou S, Kalozoumis P, Pflaum M, Iablonskii P, Glasmacher B, Harder M, Hegermann J, Wrede C, Tudorache I, Cebotari S, Hilfiker A, Haverich A, Korossis S. Development and Characterization of a Porcine Mitral Valve Scaffold for Tissue Engineering. J Cardiovasc Transl Res. 2017/05/04 ed2017. p. 374-90.

Steffen L, Ruppert C, Hoymann HG, Funke M, Ebener S, Kloth C, Muhlfeld C, Ochs M, Knudsen L, Lopez-Rodriguez E. Surfactant replacement therapy reduces acute lung injury and collapse induration-related lung remodeling in the bleomycin model. Am J Physiol Lung Cell Mol Physiol. 2017/04/30 ed2017. p. L313-L27.

Schneider JP, Arkenau M, Knudsen L, Wedekind D, Ochs M. Lung remodeling in aging surfactant protein D deficient mice. Ann Anat. 2017/02/12 ed2017. p. 158-75.

Schipke J, Brandenberger C, Rajces A, Manninger M, Alogna A, Post H, Muhlfeld C. Assessment of cardiac fibrosis: a morphometric method comparison for collagen quantification. J Appl Physiol (1985). 2017/01/28 ed2017. p. 1019-30.

Eisenberg T, Abdellatif M, Zimmermann A, Schroeder S, Pendl T, Harger A, Stekovic S, Schipke J, Magnes C, Schmidt A, Ruckenstuhl C, Dammbrueck C, Gross AS, Herbst V, Carmona-Gutierrez D, Pietrocola F, Pieber TR, Sigrist SJ, Linke WA, Muhlfeld C, Sadoshima J, Dengjel J, Kiechl S, Kroemer G, Sedej S, Madeo F. Dietary spermidine for lowering high blood pressure. Autophagy. 2017/01/25 ed2017. p. 767-9.

Schmiedl A, Roolfs T, Tutdibi E, Gortner L, Monz D. Influence of prenatal hypoxia and postnatal hyperoxia on morphologic lung maturation in mice. PLoS One: Public Library of Science; 2017. p. e0175804.

Jörger A, Acevedo C, Busley D, Ganter M, Schmiedl A, Humann-Ziehank E. Stereological and biophysical characteristics of the ovine surfactant system and its changes caused by ovine pulmonary adenocarcinoma. Research in Veterinary Science2017. p. 332-40.

Brandenberger C, Kling KM, Vital M, Mühlfeld C. The Role of Pulmonary and Systemic Immunosenescence in Acute Lung Injury. A&D. 2017:0-.

Beers MF, Knudsen L, Tomer Y, Maronn J, Zhao M, Ochs M, Mulugeta S. Aberrant lung remodeling in a mouse model of surfactant dysregulation induced by modulation of the Abca3 gene. Annals of Anatomy - Anatomischer Anzeiger2017. p. 135-46.

Schrimpf C, Wrede C, Glage S, Hegermann J, Backhaus S, Blasczyk R, Heuft HG, Muller T. Differentiation of induced pluripotent stem cell-derived neutrophil granulocytes from common marmoset monkey (Callithrix jacchus). Transfusion. 2017;57(1):60-9.

Muhlfeld C, Madsen J, Mackay RM, Schneider JP, Schipke J, Lutz D, Birkelbach B, Knudsen L, Botto M, Ochs M, Clark H. Effect of irradiation/bone marrow transplantation on alveolar epithelial type II cells is aggravated in surfactant protein D deficient mice. Histochemistry and cell biology. 2017;147(1):49-61.

Knudsen L, Ruppert C, Ochs M. Tissue remodelling in pulmonary fibrosis. Cell Tissue Res. 2017;367(3):607-26.

Kling KM, Lopez-Rodriguez E, Pfarrer C, Muhlfeld C, Brandenberger C. Aging exacerbates acute lung injury-induced changes of the air-blood barrier, lung function, and inflammation in the mouse. Am J Physiol Lung Cell Mol Physiol. 2017;312(1):L1-L12.

Grothausmann R, Knudsen L, Ochs M, Muhlfeld C. Digital 3D reconstructions using histological serial sections of lung tissue including the alveolar capillary network. Am J Physiol Lung Cell Mol Physiol. 2017;312(2):L243-L57.

Ebener S, Barnowski S, Wotzkow C, Marti TM, Lopez-Rodriguez E, Crestani B, Blank F, Schmid RA, Geiser T, Funke M. Toll-like receptor 4 activation attenuates profibrotic response in control lung fibroblasts but not in fibroblasts from patients with IPF. Am J Physiol Lung Cell Mol Physiol. 2017;312(1):L42-L55.

2016

Weidemann M, Schuster-Gossler K, Stauber M, Wrede C, Hegermann J, Ott T, Boldt K, Beyer T, Serth K, Kremmer E, Blum M, Ueffing M, Gossler A. CFAP157 is a murine downstream effector of FOXJ1 that is specifically required for flagellum morphogenesis and sperm motility. Development. 2016;143(24):4736-48.

Weber N, Schwanke K, Greten S, Wendland M, Iorga B, Fischer M, Geers-Knorr C, Hegermann J, Wrede C, Fiedler J, Kempf H, Franke A, Piep B, Pfanne A, Thum T, Martin U, Brenner B, Zweigerdt R, Kraft T. Stiff matrix induces switch to pure beta-cardiac myosin heavy chain expression in human ESC-derived cardiomyocytes. Basic research in cardiology. 2016;111(6):68.

Schlosser A, Pilecki B, Hemstra LE, Kejling K, Kristmannsdottir GB, Wulf-Johansson H, Moeller JB, Fuchtbauer EM, Nielsen O, Kirketerp-Moller K, Dubey LK, Hansen PB, Stubbe J, Wrede C, Hegermann J, Ochs M, Rathkolb B, Schrewe A, Bekeredjian R, Wolf E, Gailus-Durner V, Fuchs H, Hrabe de Angelis M, Lindholt JS, Holmskov U, Sorensen GL. MFAP4 Promotes Vascular Smooth Muscle Migration, Proliferation and Accelerates Neointima Formation. Arteriosclerosis, thrombosis, and vascular biology. 2016;36(1):122-33.

Schipke J, Grimm C, Arnstein G, Kockskamper J, Sedej S, Muhlfeld C. Cardiomyocyte loss is not required for the progression of left ventricular hypertrophy induced by pressure overload in female mice. J Anat. 2016;229(1):75-81.

Reiter U, Reiter G, Manninger M, Adelsmayr G, Schipke J, Alogna A, Rajces A, Stalder AF, Greiser A, Muhlfeld C, Scherr D, Post H, Pieske B, Fuchsjager M. Early-stage heart failure with preserved ejection fraction in the pig: a cardiovascular magnetic resonance study. J Cardiovasc Magn Reson. 2016;18(1):63.

Ong CB, Kumagai K, Brooks PT, Brandenberger C, Lewandowski RP, Jackson-Humbles DN, Nault R, Zacharewski TR, Wagner JG, Harkema JR. Ozone-Induced Type 2 Immunity in Nasal Airways. Development and Lymphoid Cell Dependence in Mice. Am J Respir Cell Mol Biol. 2016;54(3):331-40.

Ochs M, Knudsen L, Hegermann J, Wrede C, Grothausmann R, Muhlfeld C. Using electron microscopes to look into the lung. Histochemistry and cell biology. 2016;146(6):695-707.

Lopez-Rodriguez E, Gay-Jordi G, Mucci A, Lachmann N, Serrano-Mollar A. Lung surfactant metabolism: early in life, early in disease and target in cell therapy. Cell Tissue Res. 2016.

Lopez-Rodriguez E, Boden C, Echaide M, Perez-Gil J, Kolb M, Gauldie J, Maus UA, Ochs M, Knudsen L. Surfactant dysfunction during overexpression of TGF-beta1 precedes profibrotic lung remodeling in vivo. Am J Physiol Lung Cell Mol Physiol. 2016;310(11):L1260-71.

Kuebler WM, Wittenberg C, Lee WL, Reppien E, Goldenberg NM, Lindner K, Gao Y, Winoto-Morbach S, Drab M, Muhlfeld C, Dombrowsky H, Ochs M, Schutze S, Uhlig S. Thrombin stimulates albumin transcytosis in lung microvascular endothelial cells via activation of acid sphingomyelinase. Am J Physiol Lung Cell Mol Physiol. 2016;310(8):L720-32.

Kruger M, Decker S, Schneider JP, Haverich A, Schega O. [Surgical treatment of acute mediastinitis]. Chirurg. 2016;87(6):478-85.

Kellner M, Heidrich M, Lorbeer RA, Antonopoulos GC, Knudsen L, Wrede C, Izykowski N, Grothausmann R, Jonigk D, Ochs M, Ripken T, Kuhnel MP, Meyer H. A combined method for correlative 3D imaging of biological samples from macro to nano scale. Scientific reports. 2016;6:35606.

Funke M, Knudsen L, Lagares D, Ebener S, Probst CK, Fontaine BA, Franklin A, Kellner M, Kuhnel M, Matthieu S, Grothausmann R, Chun J, Roberts JD, Jr., Ochs M, Tager AM. Lysophosphatidic Acid Signaling through the Lysophosphatidic Acid-1 Receptor Is Required for Alveolarization. Am J Respir Cell Mol Biol. 2016;55(1):105-16.

Eisenberg T, Abdellatif M, Schroeder S, Primessnig U, Stekovic S, Pendl T, Harger A, Schipke J, Zimmermann A, Schmidt A, Tong M, Ruckenstuhl C, Dammbrueck C, Gross AS, Herbst V, Magnes C, Trausinger G, Narath S, Meinitzer A, Hu Z, Kirsch A, Eller K, Carmona-Gutierrez D, Buttner S, Pietrocola F, Knittelfelder O, Schrepfer E, Rockenfeller P, Simonini C, Rahn A, Horsch M, Moreth K, Beckers J, Fuchs H, Gailus-Durner V, Neff F, Janik D, Rathkolb B, Rozman J, de Angelis MH, Moustafa T, Haemmerle G, Mayr M, Willeit P, von Frieling-Salewsky M, Pieske B, Scorrano L, Pieber T, Pechlaner R, Willeit J, Sigrist SJ, Linke WA, Muhlfeld C, Sadoshima J, Dengjel J, Kiechl S, Kroemer G, Sedej S, Madeo F. Cardioprotection and lifespan extension by the natural polyamine spermidine. Nat Med. 2016;22(12):1428-38.

Busley D, Ochs M, Hoeltig D, Ganter M, Acevedo C, Schmiedl A, Hennig-Pauka I. Characterization of surfactant alterations in pigs infected with Actinobacillus pleuropneumoniae. Exp Lung Res. 2016;42(1):1-13.

Brandenberger C, Muhlfeld C. Mechanisms of lung aging. Cell Tissue Res. 2016.

Bougioukas I, Didilis V, Emigholz J, Waldmann-Beushausen R, Stojanovic T, Muhlfeld C, Schoendube FA, Danner BC. The effect of amifostine on lung ischaemia-reperfusion injury in rats. Interact Cardiovasc Thorac Surg. 2016;23(2):273-9.

Beers MF, Knudsen L, Tomer Y, Maronn J, Zhao M, Ochs M, Mulugeta S. Aberrant lung remodeling in a mouse model of surfactant dysregulation induced by modulation of the Abca3 gene. Ann Anat. 2016.

Bates MA, Brandenberger C, Langohr, II, Kumagai K, Lock AL, Harkema JR, Holian A, Pestka JJ. Silica-Triggered Autoimmunity in Lupus-Prone Mice Blocked by Docosahexaenoic Acid Consumption. PloS one. 2016;11(8):e0160622.

Baisantry A, Bhayana S, Wrede C, Hegermann J, Haller H, Melk A, Schmitt R. The impact of autophagy on the development of senescence in primary tubular epithelial cells. Cell Cycle. 2016;15(21):2973-9.

Baisantry A, Bhayana S, Rong S, Ermeling E, Wrede C, Hegermann J, Pennekamp P, Sorensen-Zender I, Haller H, Melk A, Schmitt R. Autophagy Induces Prosenescent Changes in Proximal Tubular S3 Segments. Journal of the American Society of Nephrology : JASN. 2016;27(6):1609-16.

Ahuja S, Knudsen L, Chillappagari S, Henneke I, Ruppert C, Korfei M, Gochuico BR, Bellusci S, Seeger W, Ochs M, Guenther A, Mahavadi P. MAP1LC3B overexpression protects against Hermansky-Pudlak syndrome type-1-induced defective autophagy in vitro. Am J Physiol Lung Cell Mol Physiol. 2016;310(6):L519-31.

2015

Willfuhr A, Brandenberger C, Piatkowski T, Grothausmann R, Nyengaard JR, Ochs M, Muhlfeld C. Estimation of the Number of Alveolar Capillaries by the Euler Number (Euler-Poincare Characteristic). Am J Physiol Lung Cell Mol Physiol. 2015:ajplung 00410 2014.

Vital M, Harkema JR, Rizzo M, Tiedje J, Brandenberger C. Alterations of the Murine Gut Microbiome with Age and Allergic Airway Disease. J Immunol Res. 2015;2015:892568.

Schipke J, Roloff K, Kuhn M, Muhlfeld C. Systemic, but Not Cardiomyocyte-Specific, Deletion of the Natriuretic Peptide Receptor Guanylyl Cyclase a Increases Cardiomyocyte Number in Neonatal Mice. Histochem Cell Biol. 2015;144(4):365-75. Epub 2015/06/11.

Muhlfeld C, Hegermann J, Wrede C, Ochs M. A Review of Recent Developments and Applications of Morphometry/Stereology in Lung Research. Am J Physiol Lung Cell Mol Physiol. 2015;309(6):L526-36.

McDonough JE, Knudsen L, Wright AC, Elliott W, Ochs M, Hogg JC. Regional Differences in Alveolar Density in the Human Lung Are Related to Lung Height. J Appl Physiol (1985). 2015;118(11):1429-34.

Lutz D, Gazdhar A, Lopez-Rodriguez E, Ruppert C, Mahavadi P, Gunther A, Klepetko W, Bates JH, Smith B, Geiser T, Ochs M, Knudsen L. Alveolar Derecruitment and Collapse Induration as Crucial Mechanisms in Lung Injury and Fibrosis. Am J Respir Cell Mol Biol. 2015;52(2):232-43.

Kupsch A, Lange A, Hentschel MP, Luck S, Schmidt V, Grothausmann R, Hilger A, Manke I. Missing Wedge Computed Tomography by Iterative Algorithm Directt. J Microsc. 2015.

Knudsen L, Atochina-Vasserman EN, Massa CB, Birkelbach B, Guo CJ, Scott P, Haenni B, Beers MF, Ochs M, Gow AJ. The Role of Inducible Nitric Oxide Synthase for Interstitial Remodeling of Alveolar Septa in Surfactant Protein D-Deficient Mice. Am J Physiol Lung Cell Mol Physiol. 2015;309(9):L959-69. Epub 2015/09/01.

Kellner M, Wehling J, Warnecke G, Heidrich M, Izykowski N, Vogel-Claussen J, Lorbeer RA, Antonopoulos G, Janciauskiene S, Grothausmann R, Knudsen L, Ripken T, Meyer H, Kreipe H, Ochs M, Jonigk D, Kuhnel MP. Correlating 3d Morphology with Molecular Pathology: Fibrotic Remodelling in Human Lung Biopsies. Thorax. 2015.

Katsirntaki K, Mauritz C, Olmer R, Schmeckebier S, Sgodda M, Puppe V, Eggenschwiler R, Duerr J, Schubert SC, Schmiedl A, Ochs M, Cantz T, Salwig I, Szibor M, Braun T, Rathert C, Martens A, Mall MA, Martin U. Bronchoalveolar Sublineage Specification of Pluripotent Stem Cells: Effect of Dexamethasone Plus Camp-Elevating Agents and Keratinocyte Growth Factor. Tissue Eng Part A. 2015;21(3-4):669-82.

Jonigk D, Izykowski N, Rische J, Braubach P, Kuhnel M, Warnecke G, Lippmann T, Kreipe H, Haverich A, Welte T, Gottlieb J, Laenger F. Molecular Profiling in Lung Biopsies of Human Pulmonary Allografts to Predict Chronic Lung Allograft Dysfunction. Am J Pathol. 2015;185(12):3178-88.

Holm AT, Wulf-Johansson H, Hvidsten S, Jorgensen PT, Schlosser A, Pilecki B, Ormhoj M, Moeller JB, Johannsen C, Baun C, Andersen T, Schneider JP, Hegermann J, Ochs M, Gotz AA, Schulz H, de Angelis MH, Vestbo J, Holmskov U, Sorensen GL. Characterization of Spontaneous Air Space Enlargement in Mice Lacking Microfibrillar-Associated Protein 4. Am J Physiol Lung Cell Mol Physiol. 2015;308(11):L1114-24.

Grothausmann R, Kellner M, Heidrich M, Lorbeer RA, Ripken T, Meyer H, Kuehnel MP, Ochs M, Rosenhahn B. Method for 3d Airway Topology Extraction. Comput Math Methods Med. 2015;2015:127010.

Brandenberger C, Ochs M, Mühlfeld C. Assessing Particle and Fiber Toxicology in the Respiratory System: The Stereology Toolbox. Particle and Fibre Toxicology. 2015;12(1):1-15.

Brandenberger C, Hotchkiss JA, Krieger SM, Pottenger LH, Harkema JR. Inhalation Exposure to Ethylene Induces Eosinophilic Rhinitis and Nasal Epithelial Remodeling in Fischer 344 Rats. Chem Biol Interact. 2015.

Boning A, Rohrbach S, Kohlhepp L, Heep M, Hagmuller S, Niemann B, Muhlfeld C. Differences in Ischemic Damage between Young and Old Hearts--Effects of Blood Cardioplegia. Exp Gerontol. 2015;67:3-8. Epub 2015/04/29.

Birkelbach B, Lutz D, Ruppert C, Henneke I, Lopez-Rodriguez E, Gunther A, Ochs M, Mahavadi P, Knudsen L. Linking Progression of Fibrotic Lung Remodeling and Ultrastructural Alterations of Alveolar Epithelial Type Ii Cells in the Amiodarone Mouse Model. Am J Physiol Lung Cell Mol Physiol. 2015;309(1):L63-75.

Becker S, Steinmeyer J, Avsar M, Hoffler K, Salman J, Haverich A, Warnecke G, Ochs M, Schnapper A. Evaluating Acellular Versus Cellular Perfusate Composition During Prolonged Ex Vivo Lung Perfusion after Initial Cold Ischaemia for 24 Hours. Transpl Int. 2015.

Baisantry A, Bhayana S, Rong S, Ermeling E, Wrede C, Hegermann J, Pennekamp P, Sorensen-Zender I, Haller H, Melk A, Schmitt R. Autophagy Induces Prosenescent Changes in Proximal Tubular S3 Segments. J Am Soc Nephrol. 2015.

Schneider JP, Pedersen L, Muhlfeld C, Ochs M. Staining Histological Lung Sections with Sudan Black B or Sudan Iii for Automated Identification of Alveolar Epithelial Type Ii Cells. Acta Histochem. 2015;117(8):675-80. Epub 2015/11/13.

2014

Winkler C, Bahlmann O, Viereck J, Knudsen L, Wedekind D, Hoymann HG, Madsen J, Thum T, Hohlfeld JM, Ochs M. Impact of a Met(11)Thr Single Nucleotide Polymorphism of Surfactant Protein D on Allergic Airway Inflammation in a Murine Asthma Model. Exp Lung Res. 2014;40(4):154-63.

Tahedl D, Wirkes A, Tschanz SA, Ochs M, Muhlfeld C. How Common Is the Lipid Body-Containing Interstitial Cell in the Mammalian Lung? Am J Physiol Lung Cell Mol Physiol. 2014;307(5):L386-94. Epub 2014/06/29.

Slottosch I, Liakopoulos O, Kuhn E, Deppe A, Lopez-Pastorini A, Schwarz D, Neef K, Choi Y-H, Jung K, Mühlfeld C, Wahlers T. Controlled Lung Reperfusion to Reduce Pulmonary Ischaemia/Reperfusion Injury after Cardiopulmonary Bypass in a Porcine Model. Interactive CardioVascular and Thoracic Surgery. 2014;19(6):962-70.

Schneider JP, Ochs M. Alterations of Mouse Lung Tissue Dimensions During Processing for Morphometry: A Comparison of Methods. Am J Physiol Lung Cell Mol Physiol. 2014;306(4):L341-50.

Schipke J, Mayhew TM, Muhlfeld C. Allometry of Left Ventricular Myocardial Innervation. J Anat. 2014;224(4):518-26.

Schipke J, Banmann E, Nikam S, Voswinckel R, Kohlstedt K, Loot AE, Fleming I, Muhlfeld C. The Number of Cardiac Myocytes in the Hypertrophic and Hypotrophic Left Ventricle of the Obese and Calorie-Restricted Mouse Heart. J Anat. 2014;225(5):539-47.

Rudat C, Grieskamp T, Rohr C, Airik R, Wrede C, Hegermann J, Herrmann BG, Schuster-Gossler K, Kispert A. Upk3b Is Dispensable for Development and Integrity of Urothelium and Mesothelium. PLoS One. 2014;9(11):e112112.

Ochs M. Estimating Structural Alterations in Animal Models of Lung Emphysema. Is There a Gold Standard? Ann Anat. 2014;196(1):26-33.

Muhlfeld C. Quantitative Morphology of the Vascularisation of Organs: A Stereological Approach Illustrated Using the Cardiac Circulation. Ann Anat. 2014;196(1):12-9.

Mohamed BA, Barakat AZ, Held T, Elkenani M, Muhlfeld C, Manner J, Adham IM. Respiratory Distress and Early Neonatal Lethality in Hspa4l/Hspa4 Double-Mutant Mice. Am J Respir Cell Mol Biol. 2014;50(4):817-24.

Minocchieri S, Knoch S, Schoel WM, Ochs M, Nelle M. Nebulizing Poractant Alfa Versus Conventional Instillation: Ultrastructural Appearance and Preservation of Surface Activity. Pediatr Pulmonol. 2014;49(4):348-56.

Mahavadi P, Henneke I, Ruppert C, Knudsen L, Venkatesan S, Liebisch G, Chambers RC, Ochs M, Schmitz G, Vancheri C, Seeger W, Korfei M, Guenther A. Altered Surfactant Homeostasis and Alveolar Epithelial Cell Stress in Amiodarone-Induced Lung Fibrosis. Toxicol Sci. 2014;142(1):285-97.

Mahavadi P, Henneke I, Knudsen L, Ruppert C, Venkatesan S, Liebisch G, Chambers RC, Piskulak K, Ochs M, Schmitz G, Vancheri C, Seeger W, Korfei M, Guenther A. Regulation of Macroautophagy in Amiodarone Induced Pulmonary Fibrosis. Pneumologie. 2014;68(02):A18.

Lange A, Hentschel MP, Kupsch A, Hilger A, Manke I, Lück S, Ulm VS, Grothausmann R. Reduction of Missing Wedge Artifacts in Computerised Tomography by Directt. Materials Testing. 2014;56(9):716-21.

Korner C, Kuchenbuch T, Pfeil U, Jung K, Padberg W, Kummer W, Muhlfeld C, Grau V. Low-Dose Adrenomedullin-2/Intermedin(8-47) Reduces Pulmonary Ischemia/Reperfusion Injury. Peptides. 2014;62:49-54.

Knudsen L, Atochina-Vasserman EN, Guo CJ, Scott PA, Haenni B, Beers MF, Ochs M, Gow AJ. Nos2 Is Critical to the Development of Emphysema in Sftpd Deficient Mice but Does Not Affect Surfactant Homeostasis. PLoS One. 2014;9(1):e85722.

Johnson L, Montgomery JB, Schneider JP, Townsend HG, Ochs M, Singh B. Morphometric Examination of the Equine Adult and Foal Lung. Anat Rec (Hoboken). 2014;297(10):1950-62.

Herrmann G, Knudsen L, Madershahian N, Muhlfeld C, Frank K, Rahmanian P, Wahlers T, Wittwer T, Ochs M. Effects of Exogenous Surfactant on the Non-Heart-Beating Donor Lung Graft in Experimental Lung Transplantation - a Stereological Study. J Anat. 2014;224(5):594-602.

Chowdhury A, Herzog C, Hasselbach L, Khouzani HL, Zhang J, Hammerschmidt M, Rudat C, Kispert A, Gaestel M, Menon MB, Tudorache I, Hilfiker-Kleiner D, Muhlfeld C, Schmitto JD, Muller M, Theilmeier G. Expression of Fibulin-6 in Failing Hearts and Its Role for Cardiac Fibroblast Migration. Cardiovasc Res. 2014;103(4):509-20.

Boning A, Hagmuller S, Heep M, Rohrbach S, Niemann B, Muhlfeld C. Is Warm or Cold Calafiore Blood Cardioplegia Better? Hemodynamic, Metabolic, and Electron Microscopic Differences. Thorac Cardiovasc Surg. 2014;62(8):683-9.

Bang C, Batkai S, Dangwal S, Gupta SK, Foinquinos A, Holzmann A, Just A, Remke J, Zimmer K, Zeug A, Ponimaskin E, Schmiedl A, Yin X, Mayr M, Halder R, Fischer A, Engelhardt S, Wei Y, Schober A, Fiedler J, Thum T. Cardiac Fibroblast-Derived Microrna Passenger Strand-Enriched Exosomes Mediate Cardiomyocyte Hypertrophy. J Clin Invest. 2014;124(5):2136-46. Epub 2014/04/20.

Wittwer T, Rahmanian P, Choi YH, Zeriouh M, Karavidic S, Neef K, Christmann A, Piatkowski T, Schnapper A, Ochs M, Muhlfeld C, Wahlers T. Mesenchymal Stem Cell Pretreatment of Non-Heart-Beating-Donors in Experimental Lung Transplantation. J Cardiothorac Surg. 2014;9:151.

2013

Vasilescu DM, Klinge C, Knudsen L, Yin L, Wang G, Weibel ER, Ochs M, Hoffman EA. Stereological Assessment of Mouse Lung Parenchyma Via Nondestructive, Multiscale Micro-Ct Imaging Validated by Light Microscopic Histology. J Appl Physiol (1985). 2013;114(6):716-24. Epub 2012/12/25.

Timpel M, Wanderka N, Grothausmann R, Banhart J. Distribution of Fe-Rich Phases in Eutectic Grains of Sr-Modified Al–10 Wt.% Si–0.1 Wt.% Fe Casting Alloy. Journal of Alloys and Compounds. 2013;558:18-25.

Schneider JP, Ochs M. Stereology of the Lung. Methods Cell Biol. 2013;113:257-94.

Schmeckebier S, Mauritz C, Katsirntaki K, Sgodda M, Puppe V, Duerr J, Schubert SC, Schmiedl A, Lin Q, Palecek J, Draeger G, Ochs M, Zenke M, Cantz T, Mall MA, Martin U. Keratinocyte Growth Factor and Dexamethasone Plus Elevated Camp Levels Synergistically Support Pluripotent Stem Cell Differentiation into Alveolar Epithelial Type Ii Cells. Tissue Eng Part A. 2013;19(7-8):938-51.

Piatkowski T, Muhlfeld C, Borchardt T, Braun T. Reconstitution of the Myocardium in Regenerating Newt Hearts Is Preceded by Transient Deposition of Extracellular Matrix Components. Stem Cells Dev. 2013;22(13):1921-31.

Patra C, van Amerongen MJ, Ghosh S, Ricciardi F, Sajjad A, Novoyatleva T, Mogha A, Monk KR, Muhlfeld C, Engel FB. Organ-Specific Function of Adhesion G Protein-Coupled Receptor Gpr126 Is Domain-Dependent. Proc Natl Acad Sci U S A. 2013;110(42):16898-903.

Ochs M, Muhlfeld C. Quantitative Microscopy of the Lung: A Problem-Based Approach. Part 1: Basic Principles of Lung Stereology. Am J Physiol Lung Cell Mol Physiol. 2013;305(1):L15-22.

Ochs M. Rebuilding the Lung: Signals for a Complex Architectural Task. J Appl Physiol (1985). 2013;114(8):959-60.

Muhlfeld C, Schipke J, Schmidt A, Post H, Pieske B, Sedej S. Hypoinnervation Is an Early Event in Experimental Myocardial Remodelling Induced by Pressure Overload. J Anat. 2013;222(6):634-44.

Muhlfeld C, Ochs M. Quantitative Microscopy of the Lung: A Problem-Based Approach. Part 2: Stereological Parameters and Study Designs in Various Diseases of the Respiratory Tract. Am J Physiol Lung Cell Mol Physiol. 2013;305(3):L205-21.

Muhlfeld C, Knudsen L, Ochs M. Stereology and Morphometry of Lung Tissue. Methods Mol Biol. 2013;931:367-90.

Monz D, Tutdibi E, Mildau C, Shen J, Kasoha M, Laschke MW, Roolfs T, Schmiedl A, Tschernig T, Bieback K, Gortner L. Human Umbilical Cord Blood Mononuclear Cells in a Double-Hit Model of Bronchopulmonary Dysplasia in Neonatal Mice. PLoS One. 2013;8(9):e74740.

Knudsen L, Ochs K, Boxler L, Tornoe I, Lykke-Sorensen G, Mackay RM, Clark HW, Holmskov U, Ochs M, Madsen J. Surfactant Protein D (Sp-D) Deficiency Is Attenuated in Humanised Mice Expressing the Met(11)Thr Short Nucleotide Polymorphism of Sp-D: Implications for Surfactant Metabolism in the Lung. J Anat. 2013;223(6):581-92.

Kirstein MM, Boukouris AE, Pothiraju D, Buitrago-Molina LE, Marhenke S, Schutt J, Orlik J, Kuhnel F, Hegermann J, Manns MP, Vogel A. Activity of the Mtor Inhibitor Rad001, the Dual Mtor and Pi3-Kinase Inhibitor Bez235 and the Pi3-Kinase Inhibitor Bkm120 in Hepatocellular Carcinoma. Liver Int. 2013;33(5):780-93.

Kensah G, Roa Lara A, Dahlmann J, Zweigerdt R, Schwanke K, Hegermann J, Skvorc D, Gawol A, Azizian A, Wagner S, Maier LS, Krause A, Drager G, Ochs M, Haverich A, Gruh I, Martin U. Murine and Human Pluripotent Stem Cell-Derived Cardiac Bodies Form Contractile Myocardial Tissue in Vitro. Eur Heart J. 2013;34(15):1134-46.

Gortner L, Monz D, Mildau C, Shen J, Kasoha M, Laschke MW, Roolfs T, Schmiedl A, Meier C, Tutdibi E. Bronchopulmonary Dysplasia in a Double-Hit Mouse Model Induced by Intrauterine Hypoxia and Postnatal Hyperoxia: Closer to Clinical Features? Ann Anat. 2013;195(4):351-8.

Wittwer T, Madershahian N, Rahmanian P, Choi YH, Neef K, Frank K, Muller-Ehmsen J, Ochs M, Muhlfeld C, Wahlers T. Surfactant Application in Experimental Lung Transplantation. J Heart Lung Transplant. 2013;32(3):355-9.

Gazdhar A, Susuri N, Hostettler K, Gugger M, Knudsen L, Roth M, Ochs M, Geiser T. Hgf Expressing Stem Cells in Usual Interstitial Pneumonia Originate from the Bone Marrow and Are Antifibrotic. PLoS One. 2013;8(6):e65453.

Adam RJ, Michalski AS, Bauer C, Abou Alaiwa MH, Gross TJ, Awadalla MS, Bouzek DC, Gansemer ND, Taft PJ, Hoegger MJ, Diwakar A, Ochs M, Reinhardt JM, Hoffman EA, Beichel RR, Meyerholz DK, Stoltz DA. Air Trapping and Airflow Obstruction in Newborn Cystic Fibrosis Piglets. Am J Respir Crit Care Med. 2013;188(12):1434-41.

Gazdhar A, Temuri A, Knudsen L, Gugger M, Schmid RA, Ochs M, Geiser T. Targeted Gene Transfer of Hepatocyte Growth Factor to Alveolar Type Ii Epithelial Cells Reduces Lung Fibrosis in Rats. Hum Gene Ther. 2013;24(1):105-16.

Arlt T, Grothausmann R, Manke I, Markotter H, Hilger A, Kardjilov N, Totzke C, Banhart J, Kupsch A, Lange A, Hentschel MP, Kruger P, Haussmann J, Hartnig C, Wippermann K. Tomographic methods for fuel cell research. Materials Testing. 2013;55(3):207-13.

2006 - 2012

2012

Grothausmann R, Zehl G, Manke I, Fiechter S, Bogdanoff P, Dorbandt I, Kupsch A, Lange A, Hentschel M, Schumacher G, Banhart J. Three-Dimentional Characterization of Nano-Particles Catalyst. International Journal of Materials Research, Carl Hanser Verlag, 2012, 103, 135-136.

Vasilescu DM, Gao Z, Saha PK, Yin L, Wang G, Haefeli-Bleuer B, Ochs M, Weibel ER, Hoffman EA. Assessment of Morphometry of Pulmonary Acini in Mouse Lungs by Nondestructive Imaging Using Multiscale Microcomputed Tomography. Proc Natl Acad Sci U S A. 2012;109(42):17105-10. Epub 2012/10/03.

Steinwede K, Maus R, Bohling J, Voedisch S, Braun A, Ochs M, Schmiedl A, Langer F, Gauthier F, Roes J, Welte T, Bange FC, Niederweis M, Buhling F, Maus UA. Cathepsin G and Neutrophil Elastase Contribute to Lung-Protective Immunity against Mycobacterial Infections in Mice. J Immunol. 2012;188(9):4476-87.

Patra C, Talukdar S, Novoyatleva T, Velagala SR, Muhlfeld C, Kundu B, Kundu SC, Engel FB. Silk Protein Fibroin from Antheraea Mylitta for Cardiac Tissue Engineering. Biomaterials. 2012;33(9):2673-80.

Mohamed BA, Barakat AZ, Zimmermann WH, Bittner RE, Muhlfeld C, Hunlich M, Engel W, Maier LS, Adham IM. Targeted Disruption of Hspa4 Gene Leads to Cardiac Hypertrophy and Fibrosis. J Mol Cell Cardiol. 2012;53(4):459-68. Epub 2012/08/14.

Leuenberger A, Gazdhar A, Herrmann G, Ochs M, Geiser T, Knudsen L. Cell-Specific Expression of Human Hgf by Alveolar Type Ii Cells Induces Remodeling of Septal Wall Tissue in the Lung: A Morphometric Study. J Appl Physiol (1985). 2012;113(5):799-807.

Vasilescu DM, Knudsen L, Ochs M, Weibel ER, Hoffman EA. Optimized Murine Lung Preparation for Detailed Structural Evaluation Via Micro-Computed Tomography. J Appl Physiol (1985). 2012;112(1):159-66.

Kellner M, Heidrich M, Beigel R, Lorbeer RA, Knudsen L, Ripken T, Heisterkamp A, Meyer H, Kuhnel MP, Ochs M. Imaging of the Mouse Lung with Scanning Laser Optical Tomography (Slot). J Appl Physiol (1985). 2012;113(6):975-83. Epub 2012/07/17.

Grothausmann R, Gout J, Kühnel M. Voxelizer Plug-in for Blender. 2012.

Grothausmann R, Fiechter S, Beare R, Lehmann G, Kropf H, Vinod Kumar GS, Manke I, Banhart J. Automated Quantitative 3d Analysis of Faceting of Particles in Tomographic Datasets. Ultramicroscopy. 2012;122:65-75. Epub 2012/09/27.

Graulich T, Das SK, Krasteva G, Ruppert C, Wessels L, Hoefler G, Muhlfeld C. Cancer Cachexia Alters Intracellular Surfactant Metabolism but Not Total Alveolar Surface Area. Histochem Cell Biol. 2012;138(5):803-13.

Frenzel E, Korenbaum E, Hegermann J, Ochs M, Koepke J, Koczulla AR, Welte T, Kohnlein T, Janciauskiene S. Does Augmentation with Alpha1-Antitrypsin Affect Neutrophil Extracellular Traps Formation? Int J Biol Sci. 2012;8(7):1023-5.

Emmrich S, Henke K, Hegermann J, Ochs M, Reinhardt D, Klusmann JH. Mirnas Can Increase the Efficiency of Ex Vivo Platelet Generation. Ann Hematol. 2012;91(11):1673-84.

Burkhardt W, Kraft S, Ochs M, Proquitte H, Mense L, Rudiger M. Persurf, a New Method to Improve Surfactant Delivery: A Study in Surfactant Depleted Rats. PLoS One. 2012;7(10):e47923.

Alten L, Schuster-Gossler K, Beckers A, Groos S, Ulmer B, Hegermann J, Ochs M, Gossler A. Differential Regulation of Node Formation, Nodal Ciliogenesis and Cilia Positioning by Noto and Foxj1. Development. 2012;139(7):1276-84.

Knudsen L, Boxler L, Muhlfeld C, Schaefer IM, Becker L, Bussinger C, von Stietencron I, Madershahian N, Richter J, Wahlers T, Wittwer T, Ochs M. Lung Preservation in Experimental Ischemia/Reperfusion Injury and Lung Transplantation: A Comparison of Natural and Synthetic Surfactants. J Heart Lung Transplant. 2012;31(1):85-93.

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