Unit 1 - JRG Reprogramming

Lachmann N, Jagielska J, Heckl D, Brennig S, Pfaff N, Maetzig T, Modlich U, Cantz T, Gentner B, Schambach A, Moritz T. (2011) Micro RNA-150 regulated vectors allows lymphocyte-sparing transgene expression in hematopoietic gene therapy. Gene Therapy 2011, Oct 6 (epub)

Pfaff N, Fiedler J, Holzmann A, Schambach A, Moritz T, Cantz T, Thum T. MiRNA screening reveals a new miRNA family stimulating iPS cell generation via regulation of Meox2. EMBO reports 2011, Sep 23 (epub)

Pfaff N, Lachmann N, Sgodda M, Greber B, Araúzo-Bravo MJ, Kues W, Glage S, Baum C, Niemann H, Schambach A, Cantz T, Moritz T. Efficient hematopoietic redifferentiation of induced pluripotent stem cells derived from primitive murine bone marrow cells. Stem Cell Dev 2011,  Aug 24 (epub)

Hauschild J, Petersen B, Santiago Y, Queisser AL, Carnwath JW, Lucas-Hahn A, Zhang L, Meng X, Gregory PD, Schwinzer R, Cost GJ, Niemann H. Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases. Proc Natl Acad Sci U S A. 2011, 108:12013-7.

Garrels W, Mátés L, Holler S, Dalda A, Taylor U, Petersen B, Niemann H, Izsvák Z, Ivics Z, Kues WA. Germline transgenic pigs by Sleeping Beauty transposition in porcine zygotes and targeted integration in the pig genome. PLoS One. 2011,  6: e23573.

Aydin S, Grabellus F, Eisele L, Möllmann M, Ebeling P, Moritz T, Carpinteiro A, Nückel H, Göthert J, Dührsen U, Dürig J. Investigating the role of CD38 and functionally related molecular risk factors in the CLL NOD/SCID xenograft model. Eur J Haematol 2011, 87: 10-19.

Giordano FA, Sorg UR, Appelt JU, Lachmann N, Bleier S, Roeder I, Kleff V, Flasshove M, Zeller WJ, Allgayer H, von Kalle C, Fruehauf S, Moritz T, Laufs S. Clonal inventory screens uncover monoclonality following serial transplantation of MGMTP140K transduced stem cells and dose-intense chemotherapy. Hum Gene Therapy, 2011, 22: 697-710.

Iqbal K, Kues W, Baulain U, Garrels W, Herrmann D, Niemann H. Species-specific telomer length differences between blastocyst cell compartments and ectopic telomere extension in early bovine embryos by human telomerase reverse transcriptase. Biology Reproduct 2011, 84: 723-33

Nowak-Imialek M, Kues WA, Petersen B, Lucas-Hahn A, Herrmann D, Haridoss S, Oropeza M, Lemme E, Schöler HR, Carnwath JW, Niemann H. Oct4-Enhanced Green Fluorescent Protein Transgenic Pigs: A New Large Animal Model for Reprogramming Studies. Stem Cell Dev 2011, 20:1563-75

Nowak-Imalek M, Kues WA, Rudolph C, Schlegelberger B, Taylor U, Carnwath JW, Niemann H: Preferential loss of porcine chromosomes in reprogrammed interspecies cell hybrids. Cloning Stem Cells 2010, 12: 55-65

Sanchez-Aguilera A, Rattmann I, Drew DZ, Muller LU, Summey V, Lucas DM, Byrd JC, Croce CM, Gu Y, Cancelas JA, Johnston P, Moritz T, Williams DA. Involvement of RhoH GTPase in the development of B-cell chronic lymphocytic leukemia. Leukemia 2010, 24:97-104.

Funke S, Schneider IC, Glaser S, Mühlebach MD, Moritz T, Cattaneo R, Cichutek K. Pseudotyping lentiviral vectors with the wild-type measles virus glycoproteins improves titer and selectivity. Gene Ther 2009, 16: 700-5.

Kleff V, Sorg UR, Bury C, Suzuki T, Rattmann I, Jerabek-Willemsen M, Poremba C, Flasshove M, Opalka B, Trapnell B, Dirksen U, Moritz T. Gene therapyf of ßc-deficient pulmonary alveolar proteinosis (ßc-PAPf): Studies in a murine in vivo model. Mol Ther 2008, 16: 757-64.

Kues WA, Sudheer S, Herrmann D, Carnwath JW, Havlicek V, Besenfelder U, Lehrach H, Adjaye J, Niemann H. Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo. Proc Natl Acad Sci USA. 2008, 105: 19768-73

Nowak-Imialek M, Wrenzycki C, Herrmafnn D, Lucas-Hahn A, Lagutina I, Lemme E, Lazzari G, Galli C, Niemann H. Messenger RNA expression patterns of histone associated genes in bovine preimplantation embryos derived from different origins. Mol Reprod Dev 2008, 75: 731-43

Milsom MD, Jerabek-Willemsen M, Harris CE, Schambach A, Broun E, Bailey J, Jansen M, Schleimer D, Nattamai K, Wilhelm J, Watson A, Geiger H, Margison GP, Moritz T, Baum C, Thomale J, Williams DA. Reciprocal relationship between O6-methylguanine-DNA methyltransferase P140K expression level and chemoprotection of hematopoietic stem cells. Cancer Res 2008, 68: 6171-80

Unit 2 - JRG Stem Cell Biologie

Lachmann N, Jagielska J, Heckl D, Brennig S, Pfaff N, Maetzig T, Modlich U, Cantz T, Gentner B, Schambach A, Moritz T (2011). Microrna-150-Regulated Vectors Allow Lymphocyte-Sparing Transgene Expression in Hematopoietic Gene Therapy. Gene Ther. 2011 Oct 6.

Sgodda M, Möbus S, Hoepfner J, Sharma AD, Schambach A, Greber B, Ott M, Cantz T (2011). Improved Hepatic Differentiation Strategies for Human Induced Pluripotent Stem Cells. Curr Mol Medicine, in press

Gross B, Sgodda M, Rasche M, Schambach A, Göhring G, Schlegelberger B, Greber B, Linden T, Reinhardt D, Cantz T*, Klusmann JH*. (2011). Improved generation of patient-specific induced pluripotent stem cells using a chemically-defined and Matrigel-based approach. Curr Mol Medicine, in press

Pfaff N, Fiedler J, Holzmann A, Schambach A, Moritz T, Cantz T*, Thum T* (2011). MiRNA screening reveals a new miRNA family stimulating iPS cell generation via regulation of Meox2. EMBOreports, in press (*corresponding authors)

Eggenschwiler R, Loya K, Sgodda M, André F, Cantz T (2011). Hepatic Differentiation of Murine Disease-Specific Induced Pluripotent Stem Cells Allows Disease Modelling in vitro. Stem Cells International, doi: 10.4061/2011/924782, in press

Wu G*, Liu N*, Rittelmeyer I, Sharma AD, Sgodda M, Zaehres H, Bleidissel M, Greber B, Gentile L, Han DW, Rudolph C, Steinemann D, Schambach A, Ott M, Schöler HR, Cantz T (2011). Generation of Healthy Mice from Gene-Corrected Disease-Specific Induced Pluripotent Stem Cells. PLoS Biol 9(7): e1001099

Wurm M, Groß B, Sgodda M, Ständker L, Müller T, Forssmann WG, Horn PA, Blasczyk R, Cantz T (2011). Improved lentiviral gene transfer into human embryonic stem cells grown in co-culture with murine feeder and stroma cells. Biol Chem, in press

Pfaff N, Lachmann N, Kohlscheen S, Sgodda M, Araúzo-Bravo MJ, Greber B, Kues WA, Glage S, Baum C, Niemann H, Schambach A, Cantz T, Moritz T (2011). Efficient hematopoietic redifferentiation of induced pluripotent stem cells derived from primitive murine bone marrow cells. Stem Cells Dev. 2011 Jul 6. [Epub ahead of print]

Tiemann U, Sgodda M, Warlich E, Ballmaier M, Schöler HR, Schambach A, Cantz T (2011). Optimal Reprogramming Factor Stoichiometry Increases Colony Numbers and Affects Molecular Characteristics of Murine Induced Pluripotent Stem Cells. Cytometry Part A, 9(6):426-435

Terwel D, Löschmann YN, Schmidt HH, Schöler HR, Cantz T, Heneka MT (2011). Neuroinflammatory and behavioural changes in the Atp7B mutant mouse model of Wilson's disease. J Neurochem, 118(1):105-112

Sharma AD, Narain N, Händel EM, Iken M, Singhal N, Cathomen T, Manns MP, Schöler HR, Ott M, Cantz T (2011). MicroRNA-221 regulates FAS-induced fulminant liver failure. Hepatology, 53(5):1651-1661

Warlich E, Kühle J, Cantz T, Brugman MH, Mätzig T, Galla M, Filipczyk A, Halle S, Klump H, Schöler HR, Baum C, Schroeder T, and Schambach A (2011). Lentiviral Vector Design and Imaging Approaches to Visualize the Early Stages of Cellular Reprogramming. Mol Ther, 19(4):782-789

Zaehres H, Kögler G, Bleidissel M, Arauzo-Bravo M, Santourlidis S, Weinhold S, Greber B, Kim JB, Buchheiser A, Liedtke S, Graffmann N, Zhao X, Meyer H, Reinhardt P, Waclawczyk S, Ortmeier C, Uhrberg M, Schöler HR*, Cantz T*, Wernet P* (2010). Induction of pluripotency in human cord blood unrestricted somatic stem cells. Exp Hematol, 38(9):809-18 (*corresponding authors)

Wu G, Gentile L, Do JT, Cantz T, Sutter J, Psathaki K, Araúzo-Bravo MJ, Ortmeier C, Schöler H (2010). Efficient Derivation of Pluripotent Stem Cells from siRNA-Mediated Cdx2-deficient Mouse Embryos. Stem Cells Dev, E-Pub: 12 Oct 2010 (doi: 10.1089/scd.2010.0128)

Schambach A, Cantz T, Baum C, Cathomen T (2010). Reprogramming somatic cells into induced pluripotent stem cells. Expert Opinion On Biological Therapy (Review), 10(7):1089-103.

Voelkel C, Galla M, Maetzig T, Warlich E, Kuehle J, Zychlinski D, Bode J, Cantz T, Schambach A, Baum C (2010). Protein transduction from retroviral Gag precursors. PNAS, 107(17):7805-10.

Tomala M, Lavrentieva A, Moretti P, Rinas U, Kasper C, Stahl F, Schambach A, Warlich E, Martin U, Cantz T, Scheper T (2010). Preparation of bioactive soluble human leukemia inhibitory factor from recombinant Escherichia coli using thioredoxin as fusion partner. Protein Expr Purif., 73(1):51-7

Cantz T, Martin U (2010). Induced Pluripotent Stem (iPS) Cells: Characteristics and Perspectives. Advances in Biochemical Engineering/Biotechnology (Review), 123:107-26

Kriegeskotte C, Cantz T, Haberland J, Zibert A,  Haier J, Köhler G, Schöler HR, Schmidt HHJ, Arlinghaus HF. Laser Secondary Neutral Mass Spectrometry for Copper Detection in Micro-Scale Biopsies. J Mass Spec, 2009, 44(10):1417-22.

Haridass D, Yuan Q, Becker PD, Cantz T, Iken M, Rothe M, Narain N, Bock M, Nörder M, Legrand M, Wedemeyer H, Weijer K, Spits H, Manns MP, Cai J, Deng H, Di Santo JP, Guzman CA, Ott M. Repopulation efficiencies of adult hepatocytes, fetal liver progenitor cells and embryonic stem cell-derived hepatic cells in Alb-uPA mice. Am J Pathol, 2009, 175(4):1483-92

Loya K, Eggenschwiler R, Ko K, Sgodda M, André F, Bleidißel M, Schöler HR, and Cantz T. Hepatic differentiation of pluripotent stem cells, Biol Chem, 2009, 390(10):1047-1055.

Sancho-Bru P, Najimi M, Caruso M, Pawelyn K, Cantz T, Forbes SJ, Roskams T, Ott M, Gehling U, Sokal E, Verfaillie C, Muraca M. Stem and progenitor cells for liver repopulation: can we standardize the process from bench to bedside? Gut, 2009; 58(4):594-603

Cantz T, Bleidißel M, Stehling M, Schöler HR. In vitro differentiation of reprogrammed murine somatic cells into hepatic precursor cells. Biol Chem, 2008; 389:889–896

Cantz T, Key G, Bleidißel M, Brenne A, Schöler HR. Absence of Oct4 expression in somatic tumor cell lines. Stem Cells, 2008; 26:692– 697

Sharma AD*, Cantz T*, Vogel A, Schambach A, Hairdass D, Iken M, Bleidißel M, Manns MP, Schöler HR, Ott M. Murine Embryonic Stem Cell-Derived Hepatic Progenitor Cells Engraft in Recipient Livers with Limited Capacity of Liver Tissue Formation. Cell Transplantation, 2008; 17:313–323 (* equal contribution)

Unit 3 - JRG Differentation

3a

Maetzig T, Brugman MH, Bartels S, Heinz N, Kustokova O, Modlich U, Li Z, Galla M, Schiedlmeier B, Schambach A, Baum C (2011). Blood 117: 3053-3064.

Turan S, Galla M, Ernst E, Qiao J, Voelkel C, Schiedlmeier B, Zehe C, Bohde J (2011). Recombinase-mediated cassette exchange (RCME): traditional concepts and current challenges. J Mol Biol. 407: 193-221.

Heinz N, Schambach A, Galla M, Maetzig T, Baum C, Loew R, Schiedlmeier B (2011). Retroviral and transposon-based tet-regulated all-in-one vectors with reduced background expression and improved dynamic range. Hum Gene Ther. 22: 166-176.

Kustikova OS, Schiedlmeier B, Brugman MH, Stahlhut M, Bartels S, Li Z, Baum C (2009). Cell-intrinsic defects and vector-related properties cooperate to determine the incidence and consequences of insertional mutagenesis. Mol Ther 2009 17: 1537-1547

Milsom MD, Schiedlmeier B, Bailey J, Kim MO, Li D, Jansen M, Ali AM, Kirby M, Baum C, Fairbairn LJ, Williams DA (2009). Ectopic HOXB4 overcomes the inhibitory effect of tumor necrosis factor- alpha on Fanconi anemia hematopoietic stem and progenitor cells. Blood 113: 5111-5120

Chan KM, Bonde S, Klump H, Zavazava N (2008). Hematopoiesis and immunity of HOXB4-transduced embryonic stem cell-derived hematopoietic progenitor cells. Blood 111: 2953-2961.

Schiedlmeier B, Santos AC, Ribeiro A, Moncaut N, Lesinski D, Auer H, Kornacker K, Ostertag W, Baum C, Mallo M, Klump H. (2007) HOXB4 ´s road map to stem cell expansion. Proc Natl Acad Sci USA 104: 16952-16957

3b

Naujok O, Burns C, Jones PM, Lenzen S. Insulin-producing surrogate beta-cells from embryonic stem cells: are we there yet? Mol Ther. 2011 Oct;19(10):1759-68. doi: 10.1038/mt.2011.165. Epub 2011 Aug 9.

Naujok O, Kaldrack J, Taivankhuu T, Jorns A, Lenzen S. Selective removal of undifferentiated embryonic stem cells from differentiation cultures through HSV1 thymidine kinase and ganciclovir treatment. Stem Cell Rev 2010; 6: 450-461
Jörns A, Sennholz C, Naujok O, Lenzen S. Beta cell mass regulation in the rat pancreas through glucocorticoids and thyroid hormones. Pancreas 2010; 39: 1167-1172

Naujok O, Francini F., Picton S., Bailey CJ., Lenzen S., Jörns A. Changes in gene expression and morphology of mouse embryonic stem cells on differentiation into insulin-producing cells in vitro and in vivo. Diabetes Metab Res Rev 25, 464-476, 2009

Milsom MD, Schiedlmeier B, Bailey J, Kim MO, Li D, Jansen M, Ali AM, Kirby M, Baum C, Fairbairn LJ, Williams DA. Ectopic HOXB4 overcomes the inhibitory effect of tumor necrosis factor-{alpha} on Fanconi anemia hematopoietic stem and progenitor cells. Blood. 2009; Mar 6. [Epub ahead of print] PMID: 19270262.

Naujok O, Francini F, Picton S, Jorns A, Bailey CJ, Lenzen S (2008) A new experimental protocol for preferential differentiation of mouse embryonic stem cells into insulin-producing cells. Cell Transplant 17, 1231-1242.

Naujok O, Francini F, Jörns A, Lenzen S. An efficient experimental strategy for mouse ES cell differentiation and separation of a cytokeratin 19 positive population of insulin-producing cells. Cell Proliferation. 2008; 41, 607-24.

Chan KM, Bonde S, Klump H, Zavazava N. Hematopoiesis and immunity of HOXB4-transduced embryonic stem cell-derived hematopoietic progenitor cells. Blood. 2008; 111, 2953-61.

Unit 4 - JRG Stem Cell Glycans

M.K. Sethi, F.F. Buettner, A. Ashikov, V.B. Krylov, H. Takeuchi, N.E. Nifantiev, R.S. Haltiwanger, R. Gerardy-Schahn, H. Bakker, Molecular Cloning of a Xylosyltransferase that Transfers the Second Xylose to O-Glucosylated Epidermal Growth Factor Repeats of Notch, J Biol Chem, (2011).

F.F. Buettner, S.A. Konze, A. Maas, G.F. Gerlach, Proteomic and immunoproteomic characterization of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae, Proteome Sci, 9 (2011)

M.K. Sethi, F.F. Buettner, V.B. Krylov, H. Takeuchi, N.E. Nifantiev, R.S. Haltiwanger, R. Gerardy-Schahn, H. Bakker, Identification of glycosyltransferase 8 family members as xylosyltransferases acting on O-glucosylated notch epidermal growth factor repeats, J Biol Chem, 285 (2010) 1582-1586.

S.P. Galuska, M. Rollenhagen, M. Kaup, K. Eggers, I. Oltmann-Norden, M. Schiff, M. Hartmann, B. Weinhold, H. Hildebrandt, R. Geyer, M. Muhlenhoff, H. Geyer, Synaptic cell adhesion molecule SynCAM 1 is a target for polysialylation in postnatal mouse brain, Proc Natl Acad Sci U S A, 107 (2010) 10250-10255.

E.C. Schulz, D. Schwarzer, M. Frank, K. Stummeyer, M. Muhlenhoff, A. Dickmanns, R. Gerardy-Schahn, R. Ficner, Structural basis for the recognition and cleavage of polysialic acid by the bacteriophage K1F tailspike protein EndoNF, J Mol Biol, 397 (2010) 341-351.

E.C. Schulz, A. Dickmanns, H. Urlaub, A. Schmitt, M. Muhlenhoff, K. Stummeyer, D. Schwarzer, R. Gerardy-Schahn, R. Ficner, Crystal structure of an intramolecular chaperone mediating triple-beta-helix folding, Nat Struct Mol Biol, 17 (2010) 210-215.

S.P. Galuska, H. Geyer, C. Bleckmann, R.C. Rohrich, K. Maass, A.K. Bergfeld, M. Muhlenhoff, R. Geyer, Mass spectrometric fragmentation analysis of oligosialic and polysialic acids, Anal Chem, 82 (2010) 2059-2066.

H. Bakker, T. Oka, A. Ashikov, A. Yadav, M. Berger, N.A. Rana, X. Bai, Y. Jigami, R.S. Haltiwanger, J.D. Esko, R. Gerardy-Schahn, Functional UDP-xylose transport across the endoplasmic reticulum/Golgi membrane in a Chinese hamster ovary cell mutant defective in UDP-xylose Synthase, J Biol Chem, 284 (2009) 2576-2583.

K. Boztug, G. Appaswamy, A. Ashikov, A.A. Schaffer, U. Salzer, J. Diestelhorst, M. Germeshausen, G. Brandes, J. Lee-Gossler, F. Noyan, A.K. Gatzke, M. Minkov, J. Greil, C. Kratz, T. Petropoulou, I. Pellier, C. Bellanne-Chantelot, N. Rezaei, K. Monkemoller, N. Irani-Hakimeh, H. Bakker, R. Gerardy-Schahn, C. Zeidler, B. Grimbacher, K. Welte, C. Klein, A syndrome with congenital neutropenia and mutations in G6PC3, N Engl J Med, 360 (2009) 32-43.

F.F. Buettner, I.M. Bendalla, J.T. Bosse, J. Meens, J.H. Nash, E. Hartig, P.R. Langford, G.F. Gerlach, Analysis of the Actinobacillus pleuropneumoniae HlyX (FNR) regulon and identification of iron-regulated protein B as an essential virulence factor, Proteomics, 9 (2009) 2383-2398.

A. Johswich, B. Kraft, M. Wuhrer, M. Berger, A.M. Deelder, C.H. Hokke, R. Gerardy-Schahn, H. Bakker, Golgi targeting of Drosophila melanogaster beta4GalNAcTB requires a DHHC protein family-related protein as a pilot, J Cell Biol, 184 (2009) 173-183.

D. Kahlisch, F.F. Buettner, H.Y. Naim, G.F. Gerlach, Glycoprotein analysis of porcine bronchoalveolar lavage fluid reveals potential biomarkers corresponding to resistance to Actinobacillus pleuropneumoniae infection, Vet Res, 40 (2009) 60.

D. Schwarzer, K. Stummeyer, T. Haselhorst, F. Freiberger, B. Rode, M. Grove, T. Scheper, M. von Itzstein, M. Muhlenhoff, R. Gerardy-Schahn, Proteolytic release of the intramolecular chaperone domain confers processivity to endosialidase F, J Biol Chem, 284 (2009) 9465-9474.

I. Oltmann-Norden, S.P. Galuska, H. Hildebrandt, R. Geyer, R. Gerardy-Schahn, H. Geyer, M. Mühlenhoff, Impact of the polysialyltransferases ST8SiaII and ST8SiaIV on polysialic acid synthesis during postnatal mouse brain development. J Biol Chem. 2008; 283(3):1463-71.

S.P. Galuska, R. Geyer, R. Gerardy-Schahn, M. Mühlenhoff, H. Geyer, Enzyme-dependent Variations in the Polysialylation of the Neural Cell Adhesion Molecule NCAM in Vivo. J Biol Chem. 2008; 283(1):17-28.

A. Stolz, N. Haines, A. Pich, K.D. Irvine, C.H. Hokke, A.M. Deelder, R. Gerardy-Schahn, M. Wuhrer, H. Bakker, Distinct contributions of beta 4GalNAcTA and beta 4GalNAcTB to Drosophila glycosphingolipid biosynthesis. Glycoconj. J. 2008; 25, 167-175.

V. Krylov, N. Ustyuzhanina, A. Grachev, H. Bakker, N. Nifantiev, Stereoselective synthesis of the 3-aminopropyl glycosides of ?-D-Xyl-(1 -> 3)?-D-Glc and ?-D-Xyl-(1 -> 3)-?-D-Xyl-(1 -> 3)-?-D-Glc and of their corresponding N-octanoyl derivatives. Synthesis 2007; 20: 3147-3154.

S.P. Galuska, R. Geyer, M. Mühlenhoff, H. Geyer, Characterization of Oligo- and Polysialic Acids by MALDI-TOF-MS. Anal. Chem. 2007; 79, 7161-7169.

S. Curreli, Z. Arany, R. Gerardy-Schahn, D. Mann, N.M. Stamatos, Polysialylated neuropilin-2 is expressed on the surface of human dendritic cells and modulates dendritic cell-T lymphocyte interactions.J. Biol. Chem. 2007; 282, 30346-56.

Unit 5 - RG Cell Surgery

M. Heidrich, M.P. Kühnel, M. Kellner, R.A. Lorbeer, T. Lange, A. Winkel, M. Stiesch, H. Meyer, A. Heisterkamp, 3D imaging of biofilms on implants by detection of scattered light with a scanning laser optical tomograph, Biomed Opt Express, 2, 11, 2982-94, 2011

K. Kuetemeyer, A. Lucas-Hahn, B. Petersen, H. Niemann, A. Heisterkamp, Femtosecond laser-induced fusion of nonadherent cells and two-cell porcine embryos, J Biomed Opt, 16, 8, 088001, 2011

K. Kuetemeyer, G. Kensah, M. Heidrich, H. Meyer, U. Martin, I. Gruh, A. Heisterkamp, Two-photon induced collagen-crosslinking in bioartificial cardiac tissue, Biomed Opt Express, 19, 7, 15996-16007, 2011

M. Pospiech, M. Emons, K. Kuetemeyer, A. Heisterkamp, U. Morgner, Superresolved femtosecond laser nanosurgery of cells, Biomed Opt Express, 2, 2, 264-70, 2011

R.A. Lorbeer, M. Heidrich, C. Lorbeer, D.F. Ramírez Ojeda, G. Bicker, H. Meyer, A. Heisterkamp, Highly efficient 3D fluorescence microscopy with a scanning laser optical tomograph, Opt Express 19, 6, 5419-30, 2011

S. Kalies, K. Kuetemeyer, A. Heisterkamp, Mechanisms of high-order photobleaching and its relationship to intracellular ablation, Biomed Opt Express, 2, 4, 805-16, 2011

D. Heinemann, M. Schomaker, D. Motekaitis, J. Krawinkel, D. Killian, H. M. Escobar, C. Junghanß, A. Heisterkamp, Gold nanoparticle mediated cell manipulation using fs and ps laser pulses for cell perforation and transfection, Proc SPIE, 7925-18, 2011

M. Schomaker, J. Baumgart, D. Motekaitis, D. Heinemann, J. Krawinkel, M. Pangalos, W. Bintig, E. Boulais, R. Lachaine, B. St.-Louis Lalonde, A. Ngezahayo, M. Meunier, A. Heisterkamp, Mechanisms of gold nanoparticle mediated ultrashort laser cell membrane perforation, Proc SPIE, 7925, 79250F, 2011

K. Kuetemeyer, A. Lucas-Hahn, B. Petersen, P. Hassel, E. Lemme, H. Niemann, A. Heisterkamp, Combined multiphoton imaging and automated functional enucleation of porcine oocytes using femtosecond laser pulses, J Biomed Opt, 15, 4, 046006, 2010

K. Kuetemeyer, R. Rezgui, H. Lubatschowski, A. Heisterkamp, Influence of laser parameters and staining on femtosecond laser-based intracellular nanosurgery, Biomed Opt Express, 1, 2, 587-597, 2010

J. Baumgart,W. Bintig, A. Ngezahayo, H. Lubatschowski, and A. Heisterkamp, Fs-laser-induced Ca2+ concentration change during membrane perforation for cell transfection, Opt Express, 18, 3, 2219, 2010

K. Kuetemeyer, J. Baumgart, H. Lubatschowski, A. Heisterkamp, Repetition rate dependency of lowdensity plasma effects during femtosecond-laser-based surgery of biological tissue, Appl Phys B, 97, 3, 695, 2009

J. Baumgart, K. Kütemeyer, W. Bintig, A. Ngezahayo, W. Ertmer, H. Lubatschowski, and A. Heisterkamp, Repetition rate dependency of reactive oxygen species formation during femtosecond laser based cell surgery, J Biomed Opt, 14, 5, 054040, 2009

A. Ngezahayo, J. Baumgart, S. Przemeck, K. Kutemeyer, L. Kruppe, F. Witte, W. Bintig, H. Lubatschowski, A. Heisterkamp, Intracellular manipulation of single cells using ultrashort laser pulses: mitochondria and cytoskeleton, European Journal of Cell Biology, 88, 76-76, Suppl. 59, 2009

K. Kuetemeyer, A. Lucas-Hahn, B. Petersen, P. Hassel, E. Lemme, H. Niemann, A. Heisterkamp, Femtosecond laser based enucleation of porcine oocytes for somatic cell nuclear transfer, Proc SPIE, 7373, 2009

M. Schomaker, J. Baumgart, A. Ngezahayo, J. Bullerdiek,I. Nolte, H. Escobar, H. Lubatschowski, A. Heisterkamp, Nanoparticle mediated laser cell perforation, Proc SPIE, 7373, 2009

M. Schomaker, J. Baumgart, A. Ngezahayo, J. Bullerdiek,I. Nolte, H. Escobar, H. Lubatschowski, A. Heisterkamp, Plasmonic perforation of living cells using ultrashort laser pulses and gold nanoparticles, Proc SPIE, 7192, 2009

Baumgart J, Bintig W, Willenbrock S, Escobar H, Ngezahayo A, Ertmer W, Lubatschowski H, Heisterkamp A. Quantified femtosecond laser based opto-perforation of living GFSHR-17 and MTH53a cells, Opt Express, 16, 5, 3021-31, 2008

Heisterkamp A, et al. Fs-Laser Scissors for Photobleaching, Ablation in Fixed Samples and Living Cells and Studies of Cell Mechanics, Methods in Cell Biology, 82 Laser Manipulation of Cells and Tissue, 293-302, 2007 (invited)

Arnold CL, Heisterkamp A, Ertmer W, Lubatschowski H. Computational model for nonlinear plasma formation in high NA micromachining of transparent materials and biological cells, Opt Express, 15, 16, 10303-17, 2007

Baumgart J, Bintig W, Ngezahayo A, Ertmer W, Lubatschowski H, Heisterkamp A. Live cell opto-injection by femtosecond laser pulses, Proc. SPIE. Optical Interactions with Tissue and Cells XVIII, 2007; Vol 6435, p. 512ff.

Arnold CL, Heisterkamp A, Ertmer W, Lubatschowski H, Modeling of ultrashort pulse propagation and nonlinear plasma formation in transparent Kerr media using realistic initial conditions, Proc. SPIE, 2007; Commercial and Biomedical Applications of Ultrafast Lasers VIII.

Heisterkamp A, Baumgart J, Martin U, Ngezahayo A, Murua Escobar H, Nolte I, Lubatschowski H, Laser nanosurgery for stem cell research, Proceedings of LIM, 2007.

Unit 6 - SU Embyonic Stem Cells

6a

I. Bernemann, T. Mueller, R. Blasczyk, B. Glasmacher, N. Hofmann, Colonization of collagen scaffolds by adipocytes derived from mesenchymal stem cells of the common marmoset monkey, Biochemical and Biophysical Research Communications, 411 (2011) 317-322.

U. Diekmann, R. Spindler, W.F. Wolkers, B. Glasmacher, T. Muller, Cryopreservation and quality control of mouse embryonic feeder cells, Cryobiology, 63 (2011) 104-110.

K. Hanazawa, T. Mueller, T. Becker, M. Heistermann, R. Behr, E. Sasaki, Low invasive retrieval of preimplantation embryos from the common marmoset, Theriogenology, accepted (2011).

T. Muller, T. Hupfeld, J. Roessler, M. Simoni, J. Gromoll, R. Behr, Molecular cloning and functional characterization of endogenous recombinant common marmoset monkey (Callithrix jacchus) follicle-stimulating hormone, Journal of Medical Primatology, 40 (2011) 111-119.

R.R. Plentz, V. Palagani, O. Naujok, U. Diekmann, S. Glage, A. Wiedemann, A. Joerns, T. Mueller, Endocrine pancreas of the common marmoset monkey (Callithrix jacchus) displays islet architecture like in the human, Islets, (2011).

M. Wurm, B. Gross, M. Sgodda, L. Standker, T. Muller, W.G. Forssmann, P.A. Horn, R. Blasczyk, T. Cantz, Improved lentiviral gene transfer into human embryonic stem cells grown in co-culture with murine feeder and stroma cells, Biological Chemistry, 392 (2011) 887-895.

M. Wurm, A. Schambach, D. Lindemann, H. Hanenberg, L. Standker, W.G. Forssmann, R. Blasczyk, P.A. Horn, The influence of semen-derived enhancer of virus infection on the efficiency of retroviral gene transfer, Journal of Gene Medicine, 12 (2010) 137-146.

G. Fleischmann, T. Muller, R. Blasczyk, E. Sasaki, P.A. Horn, Growth characteristics of the nonhuman primate embryonic stem cell line cjes001 depending on feeder cell treatment, Cloning and stem cells, 11 (2009) 225-233.

T. Muller, G. Fleischmann, K. Eildermann, K. Matz-Rensing, P.A. Horn, E. Sasaki, R. Behr, A novel embryonic stem cell line derived from the common marmoset monkey (Callithrix jacchus) exhibiting germ cell-like characteristics, Human reproduction, 24 (2009) 1359-1372.

L. Sieron, S. Immenschuh, R. Blasczyk, B. Eiz-Vesper, The predicted protein structures of the novel DRB1*0717 and DRB1*0701 are highly related., Tissue Antigens, 74 (2009) 460-462.

M. Wurm, S. Tischer, S. Immenschuh, R. Blasczyk, B. Eiz-Vesper, The predicted protein structures of the novel DRB1*0717 and DRB1*0701 are highly related, Tissue antigens, 74 (2009) 460-462.

6b

Northrup E, Eisenblätter R, Glage S, Rudolph C, Dorsch M, Schlegelberger B, Hedrich HJ, Zschemisch NH (2011) Loss of Dnd1 facilitates the cultivation of genital ridge-derived rat embryonic germ cells. Exp Cell Res. 317(13):1885-94.

Held N, Smits BM, Gockeln R, Schubert S, Nave H, Northrup E, Cuppen E, Hedrich HJ, Wedekind D (2011) A mutation in Myo15 leads to Usher-like symptoms in LEW/Ztm-ci2 rats. PLoS One. 6(3):e15669.

Jörns A, Rath KJ, Terbish T, Arndt T, Meyer Zu Vilsendorf A, Wedekind D, Hedrich HJ, Lenzen S (2010) Diabetes prevention by immunomodulatory FTY720 treatment in the LEW.1AR1-iddm rat despite immune cell activation. Endocrinology. 151(8):3555-65.

de Buhr MF, Hedrich HJ, Westendorf AM, Obermeier F, Hofmann C, Zschemisch NH, Buer J, Bumann D, Goyert SM, Bleich A (2009) Analysis of Cd14 as a genetic modifier of experimental inflammatory bowel disease (IBD) in mice. Inflamm Bowel Dis. 15(12):1824-36.

Janus LM, Smoczek A, Hedrich HJ, Bleich A (2009) Risk assessment of minute virus of mice transmission during rederivation: detection in reproductive organs, gametes, and embryos of mice after in vivo infection. Biol Reprod. 81(5):1010-5.

Arndt T, Wedekind D, Weiss H, Tiedge M, Lenzen S, Hedrich HJ, Jörns A (2009) Prevention of spontaneous immune-mediated diabetes development in the LEW.1AR1-iddm rat by selective CD8+ T cell transfer is associated with a cytokine shift in the pancreas-draining lymph nodes. Diabetologia. 52(7):1381-90.

Barkhausen T, Tschernig T, Rosenstiel P, van Griensven M, Vonberg RP, Dorsch M, Mueller-Heine A, Chalaris A, Scheller J, Rose-John S, Seegert D, Krettek C, Waetzig GH 82011) Selective blockade of interleukin-6 trans-signaling improves survival in a murine polymicrobial sepsis model. Crit Care Med.;39(6):1407-13.

Dorsch M, Wedekind D (2010) Cryopreservation and orthotopic transplantation of rat ovaries. Methods Mol Biol. 597:301-10.

Zeckey C, Tschernig T, Hildebrand F, Frink M, Frömke C, Dorsch M, Krettek C, Barkhausen T (2010) Macrophage-activating lipopeptide-2 exerts protective effects in a murine sepsis model. Shock. 33(6):614-9.

Velaga S, Herbrand H, Friedrichsen M, Jiong T, Dorsch M, Hoffmann MW, Förster R, Pabst O (2009) Chemokine receptor CXCR5 supports solitary intestinal lymphoid tissue formation, B cell homing, and induction of intestinal IgA responses. J Immunol. 182(5):2610-9.

Unit 7 - RG Liver Regeneration

T.W. Kang, T. Yevsa, N. Woller, L. Hoenicke, T. Wuestefeld, D. Dauch, A. Hohmeyer, M. Gereke, R. Rudalska, A. Potapova, M. Iken, M. Vucur, S. Weiss, M. Heikenwalder, S. Khan, J. Gil, D. Bruder, M. Manns, P. Schirmacher, F. Tacke, M. Ott, T. Luedde, T. Longerich, S. Kubicka, L. Zender, Senescence surveillance of pre-malignant hepatocytes limits liver cancer development, Nature, 479 (2011).

E. Gurlevik, N. Woller, N. Struver, P. Schache, A. Kloos, M.P. Manns, L. Zender, F. Kuhnel, S. Kubicka, Selectivity of oncolytic viral replication prevents antiviral immune response and toxicity, but does not improve antitumoral immunity, Mol Ther, 18 (2010) 1972-1982

F. Kuhnel, E. Gurlevik, T.C. Wirth, N. Struver, N.P. Malek, M. Muller-Schilling, M.P. Manns, A. Carnero, L. Zender, S. Kubicka, Targeting of p53-transcriptional dysfunction by conditionally replicating adenovirus is not limited by p53-homologues, Mol Ther, 18 (2010) 936-946.

T. Wuestefeld, L. Zender, DLC1 and liver cancer: the Akt connection, Gastroenterology, 139 (2010) 1093-1096.

L. Zender, A. Villanueva, V. Tovar, D. Sia, D.Y. Chiang, J.M. Llovet, Cancer gene discovery in hepatocellular carcinoma, J Hepatol, 52 (2010) 921-929.

A. Bric, C. Miething, C.U. Bialucha, C. Scuoppo, L. Zender, A. Krasnitz, Z. Xuan, J. Zuber, M. Wigler, J. Hicks, R.W. McCombie, M.T. Hemann, G.J. Hannon, S. Powers, S.W. Lowe, Functional identification of tumor-suppressor genes through an in vivo RNA interference screen in a mouse lymphoma model, Cancer Cell, 16 (2009) 324-335.

E. Gurlevik, N. Woller, P. Schache, N.P. Malek, T.C. Wirth, L. Zender, M.P. Manns, S. Kubicka, F. Kuhnel, p53-dependent antiviral RNA-interference facilitates tumor-selective viral replication, Nucleic Acids Res, 37 (2009) e84.

M.J. Laska, S.W. Lowe, L. Zender, S. Hearn, U. Vogel, U.B. Jensen, A. Bric, B.A. Nexo, Enforced expression of PPP1R13L increases tumorigenesis and invasion through p53-dependent and p53-independent mechanisms, Mol Carcinog, 48 (2009) 832-842.

L.X. Liu, N.P. Lee, V.W. Chan, W. Xue, L. Zender, C. Zhang, M. Mao, H. Dai, X.L. Wang, M.Z. Xu, T.K. Lee, I.O. Ng, Y. Chen, H.F. Kung, S.W. Lowe, R.T. Poon, J.H. Wang, J.M. Luk, Targeting cadherin-17 inactivates Wnt signaling and inhibits tumor growth in liver carcinoma, Hepatology, 50 (2009) 1453-1463.

O. Ma, W.W. Cai, L. Zender, T. Dayaram, J. Shen, A.J. Herron, S.W. Lowe, T.K. Man, C.C. Lau, L.A. Donehower, MMP13, Birc2 (cIAP1), and Birc3 (cIAP2), amplified on chromosome 9, collaborate with p53 deficiency in mouse osteosarcoma progression, Cancer Res, 69 (2009) 2559-2567.

P. Schache, E. Gurlevik, N. Struver, N. Woller, N. Malek, L. Zender, M. Manns, T. Wirth, F. Kuhnel, S. Kubicka, VSV virotherapy improves chemotherapy by triggering apoptosis due to proteasomal degradation of Mcl-1, Gene Ther, 16 (2009) 849-861.

M.Z. Xu, T.J. Yao, N.P. Lee, I.O. Ng, Y.T. Chan, L. Zender, S.W. Lowe, R.T. Poon, J.M. Luk, Yes-associated protein is an independent prognostic marker in hepatocellular carcinoma, Cancer, 115 (2009) 4576-4585.

L. Zender, K.L. Rudolph, Keeping your senescent cells under control, Aging (Albany NY), 1 (2009) 438-441.

Schaetzlein S, Kodandaramireddy NR, Ju Z, Lechel A, Stepczynska A, Lilli DR,Clark AB, Rudolph C, Kuhnel F, Wei K, Schlegelberger B, Schirmacher P, Kunkel TA,Greenberg RA, Edelmann W, Rudolph KL. Exonuclease-1 deletion impairs DNA damage signaling and prolongs lifespan of telomere-dysfunctional mice. Cell. 2007; Sep 7;130(5):863-77.

Zender L, Xue W, Zuber J, Semighini CP, Krasnitz A, Ma, B, Zender P, Kubicka S, Luk JM, Schirmacher P, McCombie RW, Wigler M, Hicks J, Hannon GJ, Powers S, Lowe SW. An oncogenomics-based in vivo RNAi screen identifies new tumor suppressors in liver cancer, Cell, 2008; Nov.

Krizhanovsky V, Yon M, Dickins RA, Hearn S, Simon J, Miething C, Yee H, Zender L, Lowe SW. Senescence of activated stellate cells limits liver fibrosis. Cell. 2008; Aug 22;134(4):657-67.

Xue W, Krasnitz A, Lucito R, Sordella R, Vanaelst L, Cordon-Cardo C, Singer S, Kuehnel F, Wigler M, Powers S, Zender L, Lowe SW. DLC1 is a chromosome 8p tumor suppressor whose loss promotes hepatocellular carcinoma. Genes Dev. 2008; Jun 1;22(11):1439-44.

Unit 8 - W2 Proliferation Control

Puklowski, A., Homsi, Y., Chauhan, S., Keller, D., Grünwald, V., Kubicka, S., Pich, A., Manns, M.P., Hoffmann, I., Gönczy, P., and Malek, N.P., The SCFFbw5 E3-ubiquitin ligase is regulated by the APC/C and Plk4 to control centrosome duplication. Nature Cell Biology accepted 2011

Kossatz, U., Breuhahn K., Wolf B., Hardtke-Wolenski, M., Wilkens, L., Steinemann, D., Singer, S., Brass, F., Kubicka, S., Schlegelberger, B., Schirmacher, P., Manns, M.P., Singer, J.D., and Malek, N.P. The cyclin E regulator cullin 3 prevents mouse hepatic progenitor cells from becoming tumor-initiating cells. J Clin Invest. 2010 Nov 1;120(11):3820-3

Stauch, B., Simon, B., Basile, T., Schneider, G., Malek, N.P., Kalesse, M., and Carlomagno, T. Elucidation of the structure and intermolecular interactions of a reversible cyclic-peptide inhibitor of the proteasome by NMR spectroscopy and molecular modeling. 2010. Angew Chem Int Ed Engl 49:3934-3938.

Buitrago-Molina LE, Pothiraju D, Lamlé J, Marhenke S, Kossatz U, Breuhahn K, Manns MP, Malek N, Vogel A. Hepatology. 2009 Aug;50(2):500-9. Rapamycin delays tumor development in murine livers by inhibiting proliferation of hepatocytes with DNA damage. 

Gürlevik E, Woller N, Schache P, Malek NP, Wirth TC, Zender L, Manns MP, Kubicka S, Kühnel F. p53-dependent antiviral RNA-interference facilitates tumor-selective viral replication. Nucleic Acids Res. 2009 May 14. 

Ramakrishna E, Woller N, Mundt B, Knocke S, Gürlevik E, Saborowski M, Malek N, Manns MP, Wirth T, Kühnel F, Kubicka S. Antitumoral immune responses by recruitment and expansion of dendritic cells in tumors infected with telomerase-depedent oncolytic viruses. ?Cancer Res. 2009 Feb 15;69(4):1448-58. Epub 2009 Feb 3.

Nickeleit I, Zender S, Sasse F, Geffers R, Brandes G, Sörensen I, Steinmetz H, Kubicka S, Carlomagno T, Menche D, Buer J, Gossler A, Manns MP, Kalesse M, Frank R, Malek NP. Argyrin A reveals a critical role for the tumor suppressor protein p27kip1 in mediating anti-tumor activities in response to proteasome inhibition ?Cancer Cell. 2008; 14:23-35. 

McEvoy JD, Kossatz U, Malek NP, Singer JD. Constitutive turnover of cyclin E by Cul3 maintains quiescence. Mol.Cell.Biol. 2007; May;27(10):3651-66.

McEvoy, Justina D., Justina D. McEvoy, Uta Kossatz, Nisar Malek and Jeffrey D. Singer, Constitutive turnover of cyclin E by Cul3 maintains quiescence. ?Mol.Cell.Biol. 2007 May;27(10):3651-66. Epub 2007 Mar 5

Unit 9 - RG Cardiac Organogenesis

Norden J, Greulich F, Rudat C, Taketo MM, Kispert A. Wnt/{beta}-catenin signaling Maintains the mesenchymal precursor pool for murine sinus horn formation. Circ. Res. 2011;109:e42-50.

Greulich F, Rudat C, Kispert A. Mechanisms of T-box gene function in the developing heart. Cardiovasc. Res. 2011;91:212-222. 

Singh R, Kispert A. Tbx20, Smads, and the atrioventricular canal. Trends Cardiovasc. Med. 2010;20:109-114.

Grieskamp T, Rudat C, Lüdtke TH, Norden J, Kispert A. Notch signaling regulates smooth muscle differentiation of epicardium-derived cells. Circ. Res. 2011;108:813-823.

Froese N, Kattih B, Breitbart A, Grund A, Geffers R, Molkentin JD, Kispert A,  Wollert KC, Drexler H, Heineke J. GATA6 promotes angiogenic function and survival in endothelial cells by suppression of autocrine transforming growth factor beta/activin receptor-like kinase 5 signaling. J. Biol. Chem. 2011;286:5680-5690.

Aanhaanen WT, Mommersteeg MT, Norden J, Wakker V, de Gier-de Vries C, Anderson RH, Kispert A, Moorman AF, Christoffels VM. Developmental origin, growth, and three-dimensional architecture of the atrioventricular conduction axis of the mouse heart. Circ. Res. 2010;107:728-736.

Norden J, Grieskamp T, Lausch E, van Wijk B, van den Hoff MJ, Englert C, Petry M, Mommersteeg MT, Christoffels VM, Niederreither K, Kispert A. Wt1 and retinoic acid signaling in the subcoelomic mesenchyme control the development of  the pleuropericardial membranes and the sinus horns. Circ. Res. 2010;106:1212-1220. 

Christoffels VM, Smits GJ, Kispert A, Moorman AF. Development of the pacemaker tissues of the heart. Circ Res. 2010;106:240-254. 

Mommersteeg MT, Domínguez JN, Wiese C, Norden J, de Gier-de Vries C, Burch JB, Kispert A, Brown NA, Moorman AF, Christoffels VM. The sinus venosus progenitors separate and diversify from the first and second heart fields early in development. Cardiovasc Res. 2010;87:92-101. 

Singh R, Horsthuis T, Farin HF, Grieskamp T, Norden J, Petry M, Wakker V, Moorman AF, Christoffels VM, Kispert A. Tbx20 interacts with smads to confine tbx2 expression to the atrioventricular canal. Circ Res. 2009;105:442-542.

Aanhaanen WT, Brons JF, Domínguez JN, Rana MS, Norden J, Airik R, Wakker V, de Gier-de Vries C, Brown NA, Kispert A, Moorman AF, Christoffels VM. The Tbx2+ primary myocardium of the atrioventricular canal forms the atrioventricular node  and the base of the left ventricle. Circ. Res. 2009;104:1267-1274. 

Christoffels VM, Grieskamp T, Norden J, Mommersteeg MT, Rudat C, Kispert A. Tbx18 and the fate of epicardial progenitors. Nature 2009;458:E8-9.

Lüdtke TH, Christoffels VM, Petry M, Kispert A. Tbx3 promotes liver bud expansion during mouse development by suppression of cholangiocyte differentiation. Hepatology 2009;49:969-978.

Wiese C, Grieskamp T, Airik R, Mommersteeg MT, Gardiwal A, de Gier-de Vries C, Schuster-Gossler K, Moorman AF, Kispert A, Christoffels VM. Formation of the sinus node head and differentiation of sinus node myocardium are independently regulated by Tbx18 and Tbx3. Circ. Res. 2009;104:388-397.

Kobayashi K, Luo M, Zhang Y, Wilkes DC, Ge G, Grieskamp T, Yamada C, Liu TC,  Huang G, Basson CT, Kispert A, Greenspan DS, Sato TN. Secreted Frizzled-related protein 2 is a procollagen C proteinase enhancer with a role in fibrosis associated with myocardial infarction. Nat. Cell. Biol. 2009;11:46-55.

Lausch E, Hermanns P, Farin HF, Alanay Y, Unger S, Nikkel S, Steinwender C, Scherer G, Spranger J, Zabel B, Kispert A, Superti-Furga A. TBX15 mutations cause craniofacial dysmorphism, hypoplasia of scapula and pelvis, and short stature in  Cousin syndrome. Am. J. Hum. Genet. 2008;83:649-655.

Farin HF, Mansouri A, Petry M, Kispert A. T-box protein Tbx18 interacts with  the paired box protein Pax3 in the development of the paraxial mesoderm. J. Biol. Chem. 2008;283:25372-25380. 

Winkler ME, Mauritz C, Groos S, Kispert A, Menke S, Hoffmann A, Gruh I, Schwanke K, Haverich A, Martin U. Serum-free differentiation of murine embryonic  stem cells into alveolar type II epithelial cells. Cloning Stem Cells. 2008;10:49-64.

Farin HF, Bussen M, Schmidt MK, Singh MK, Schuster-Gossler K, Kispert A. Transcriptional repression by the T-box proteins Tbx18 and Tbx15 depends on Groucho corepressors. J. Biol. Chem. 2007;282:25748-25759.

Unit 10 - RG Organogenesis / Notch Signalling

A. Giros, K. Grgur, A. Gossler, M. Costell, alpha5beta1 integrin-mediated adhesion to fibronectin is required for axis elongation and somitogenesis in mice, PloS one, 6 (2011) e22002

M. Hoch, P. Fischer, B. Stapel, E. Missol-Kolka, B. Sekkali, M. Scherr, F. Favret, T. Braun, M. Eder, K. Schuster-Gossler, A. Gossler, A. Hilfiker, J.L. Balligand, H. Drexler, D. Hilfiker-Kleiner, Erythropoietin preserves the endothelial differentiation capacity of cardiac progenitor cells and reduces heart failure during anticancer therapies, Cell stem cell, 9 (2011) 131-143.

M. Clepce, A. Gossler, K. Reich, J. Kornhuber, N. Thuerauf, The relation between depression, anhedonia and olfactory hedonic estimates--a pilot study in major depression, Neuroscience letters, 471 (2010) 139-143.

B. Lenz, H.W. Klafki, T. Hillemacher, H. Frieling, M. Clepce, A. Gossler, N. Thuerauf, G. Winterer, J. Kornhuber, S. Bleich, ERK1/2 protein and mRNA levels in human blood are linked to smoking behavior, Addiction biology, (2010).

B. Lenz, H.W. Klafki, T. Hillemacher, N. Killisch, G. Schaller, H. Frieling, M. Clepce, A. Gossler, N. Thuerauf, G. Winterer, J. Kornhuber, S. Bleich, Smoking behaviour is associated with expression and phosphorylation of CREB in human buffy coat, The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum, 13 (2010) 207-215.

S.F. Rocha, S.S. Lopes, A. Gossler, D. Henrique, Dll1 and Dll4 function sequentially in the retina and pV2 domain of the spinal cord to regulate neurogenesis and create cell diversity, Developmental biology, 328 (2009) 54-65.

I. Sörensen, R. Adams, A. Gossler, DLL1 mediated Notch activation regulates endothelial identity in mouse fetal arteries. Blood. 2009; 113, 5680-88.

K. Schuster-Gossler, B. Harris, K.R. Johnson, J. Serth, A. Gossler, Notch signalling in the paraxial mesoderm is most sensitive to reduced Pofut1 levels during early mouse development. BMC Dev Biol. 2009; Jan 22;9:6.

R. Benedito, C. Roca, I. Sörensen, S. Adams, A. Gossler, M. Fruttiger, R.H. Adams, The Notch ligands Dll4 and Jagged1 have opposing effects on angiogenesis. Cell. 2009; 137, 1124-35.

A. Beckers, L. Alten, C. Viebahn, P. Andre, A. Gossler, The mouse homeobox gene Noto regulates node morphogenesis, notochordal ciliogenesis, and left-right patterning. PNAS. 2007; 104, 15765–70.

J. Feller, A. Schneider, K. Schuster-Gossler, A. Gossler, Non-cyclic Notch activity in the presomitic mesoderm demonstrates uncoupling of somite compartmentalization and boundary formation. Genes & Development. 2009; 22, 2166-71

T.B. Feyerabend, G. Terszowski, A. Tietz, C. Blum, H. Luche, A. Gossler, N.W. Gale, F. Radtke, H.J. Fehling, H.R. Rodewald, Deletion of Notch1 Converts Pro-T Cells to Dendritic Cells and Promotes Thymic B Cells by Cell-Extrinsic and Cell-Intrinsic Mechanisms. Immunity, 2009; 30, 67-79.

S. Estrach, R. Cordes, K. Hozumi, A. Gossler, F.M. Watt, Role of the Notch ligand Delta1 in embryonic and adult mouse epidermis. The Journal of Investigative Dermatology, 2008; 128, 825-832.

I. Geffers, K. Serth, G. Chapman, R. Jaekel, K. Schuster-Gossler, R. Cordes, D.B. Sparrow, E. Kremmer, S.L. Dunwoodie, T. Klein, A. Gossler, Divergent functions and distinct localization of the Notch ligands DLL1 and DLL3 in vivo. The Journal of Cell Biology, 2007; 178, 465-476.

H.T. Cheng, M. Kim, M.T. Valerius, K. Surendran, K. Schuster-Gossler, A. Gossler, A.P. McMahon, R. Kopan, Notch2, but not Notch1, is required for proximal fate acquisition in the mammalian nephron. Development, 2007; 134, 801-811.

A. Limbourg, M. Ploom, D. Elligsen, I. Sörensen, T. Ziegelhoeffer, A. Gossler, H. Drexler, F.R. Limbourg, The Notch Ligand Delta-like 1 (Dll1) is Essential for Postnatal Arteriogenesis. Circ. Res, 2007; 100, 363-371.

K. Schuster-Gossler, R. Cordes, A. Gossler, Premature myogenic differentiation and depletion of progenitor cells cause severe muscle hypotrophy in Delta1 mutants. PNAS, 2007; 104, 537-542.

Unit 11 - JRG Regenerative Agents

Heida N M, Leifheit-Nestler M, Schroeter M R, Muller J P, Cheng I F, Henkel S, Limbourg A, Limbourg F P, Alves F, Quigley J P, Ruggeri Z M, Hasenfuss G, Konstantinides S, Schafer K. (2010) Leptin enhances the potency of circulating angiogenic cells via src kinase and integrin (alpha)vbeta5: implications for angiogenesis in human obesity. Arterioscler Thromb Vasc Bio 30:200-206.

Leifheit-Nestler M, Conrad G, Heida NM, Limbourg A, Limbourg FP, Seidler T, Schroeter MR, Hasenfuss G, Konstantinides S, Schafer K. (2010) Overexpression of Integrin {beta}5 Enhances the Paracrine Properties of Circulating Angiogenic Cells via Src Kinase-Mediated Activation of STAT3. Arterioscler Thromb Vasc Biol

Limbourg FP, Drexler H. Quo vadis, progenitor? Progenitor cell homing in chronic ischemic heart disease. J Cardiovasc Pharmacol. 2009; 53:438-9.

Limbourg A, Korff T, Napp LC, Schaper W, Drexler H, Limbourg FP. Evaluation of Postnatal Arteriogenesis and Angiogenesis in a Mouse Model of Hindlimb Ischemia. Nature Protocols. 2009; 4:1737-1746.

Limbourg FP, Takeshita K, Radtke F, Bronson RT, Chin MT, Liao JK. Essential role of endothelial Notch1 in angiogenesis. Circulation. 2005.

Limbourg A, Ploom M, Elligsen D, Sorensen I, Ziegelhoeffer T, Gossler A, Drexler H, Limbourg FP. Notch ligand Delta-like 1 is essential for postnatal arteriogenesis. Circ Res. 2007.

Templin C, Kotlarz D, Faulhaber J, Schnabel S, Grote K, Salguero G, Luchtefeld M, Hiller KH, Jakob P, Naim HY, Schieffer B, Hilfiker-Kleiner D, Landmesser U, Limbourg FP, Drexler H. Ex vivo expanded hematopoietic progenitor cells improve cardiac function after myocardial infarction: role of beta-catenin transduction and cell dose. J Mol Cell Cardiol. 2008.

Tongers J, Knapp JM, Korf M, Kempf T, Limbourg A, Limbourg FP, Li Z, Fraccarollo D, Bauersachs J, Han X, Drexler H, Fiedler B, Wollert KC. Haeme oxygenase promotes progenitor cell mobilization, neovascularization, and functional recovery after critical hind-limb ischaemia in mice. Cardiovasc Res. 2008.

Unit 12 - JRG Cardiovascular Cell Therapy

Froese N, Kattih B, Breitbart A, Grund A, Geffers R, Molkentin JD, Kispert A, Wollert KC, Drexler H, and Heineke J§. GATA6 Promotes Angiogenic Function and Survival in Endothelial Cells by Suppression of Autocrine Transforming Growth Factor {beta}/Activin Receptor-like Kinase 5 Signaling. J Biol Chem 2011; 286: 5680-5690. #corresponding author

Korf-Klingebiel M, Kempf T, Schluter KD, Willenbockel C, Brod T, Heineke J, Schmidt VJ, Jantzen F, Brandes RP, Sugden PH, Drexler H, Molkentin JD, and Wollert KC. Conditional transgenic expression of fibroblast growth factor 9 in the adult mouse heart reduces heart failure mortality after myocardial infarction. Circulation 2011; 123: 504-514.

Echtermeyer F, Harendza T, Hubrich S, Lorenz A, Herzog C, Mueller M, Schmitz M, Grund A, Larmann J, Stypmann J, Schieffer B, Lichtinghagen R, Hilfiker-Kleiner D, Wollert KC, Heineke J, Theilmeier G. Syndecan-4 signaling inhibits apoptosis and controls NFAT activity during myocardial damage and remodeling. Cardiovasc Res. 2011; 92(1):123-31.

Fiedler J, Jazbutyte V, Kirchmaier BC, Gupta SK, Lorenzen J, Galuppo P, Kneitz S, Pena JTG, Sohn-Lee C, Loyer X, Soutschek J, Brand T, Tuschl T, Heineke J,  Martin U, Schulte-Merker S, Ertl G, Engelhardt S, Bauersachs J and Thum T. MicroRNA-24 regulates vascularity in a mouse model of myocardial infarction. Circulation. 2011; 124(6):720-30

Heineke J*, Auger-Messier M*, Xu J, Sargent M, York A, Welle S, Molkentin JD. Genetic deletion of myostatin from the heart prevents skeletal muscle atrophy in heart failure. Circulation. Jan 26 2010; 121(3): 419-25

Heineke J, Wollert KC, Osinska H, Sargent MA, York AJ, Robbins J, Molkentin JD. Calcineurin protects the heart in a murine model of dilated cardiomyopathy. J Mol Cell Cardiol. 2009 Oct 22.

Gunkel S*, Knoll R, Heineke J*, Hilfiker-Kleiner D. MLP: a stress sensor goes nuclear. J Mol Cell Cardiol. 2009 Oct; 47(4): 423-5.

Heineke J, Auger-Messier M, Xu J, Oka T, Sargent MA, York A, Klevitsky R, Vaikunth S, Duncan SA, Aronow BJ, Robbins J, Cromblehol TM, Molkentin JD. Cardiomyocyte GATA4 functions as a stress-responsive regulator of angiogenesis in the murine heart. J Clin Invest. Nov 2007; 117(11):3198-3210.

Unit 13 - JRG Hematopoietic Cell Therapy

Voelkel C, Galla M, Dannhauser PN, Maetzig T, Sodeik B, Schambach A, Baum C. Pseudotype-independent non-specific uptake of gammaretroviral and lentiviral particles in human cells. Hum Gene Ther. 2011 Oct 19. [Epub ahead of print] PubMed PMID: 22010882.

Maetzig T, Galla M, Baum C, Schambach A. Gammaretroviral vectors: biology, technology and application. Viruses. 2011 Jun;3(6):677-713. Epub 2011 Jun 3. PubMed PMID: 21994751; PubMed Central PMCID: PMC3185771.

Jaako P, Flygare J, Olsson K, Quere R, Ehinger M, Henson A, Ellis S, Schambach A, Baum C, Richter J, Larsson J, Bryder D, Karlsson S. Mice with ribosomal protein S19 deficiency develop bone marrow failure and symptoms like patients with Diamond-Blackfan anemia. Blood. 2011 Oct 11. [Epub ahead of print] PubMed PMID: 21989989.

Lachmann N, Jagielska J, Heckl D, Brennig S, Pfaff N, Maetzig T, Modlich U, Cantz T, Gentner B, Schambach A, Moritz T. MicroRNA-150-regulated vectors allow lymphocyte-sparing transgene expression in hematopoietic gene therapy. Gene Ther.2011 Oct 6. doi: 10.1038/gt.2011.148. [Epub ahead of print] PubMed PMID: 21975463.

Li W, Wang X, Fan W, Zhao P, Chan YC, Chen S, Zhang S, Guo X, Zhang Y, Li Y, Cai J, Qin D, Li X, Yang J, Peng T, Zychlinski D, Hoffmann D, Zhang R, Deng K, Ng KM, Menten B, Zhong M, Wu J, Li Z, Chen Y, Schambach A, Tse HF, Pei D, Esteban MA. Modeling abnormal early development with induced pluripotent stem cells from aneuploid syndromes. Hum Mol Genet. 2011 Oct 13. [Epub ahead of print] PubMed PMID: 21949351.

Pfaff N, Fiedler J, Holzmann A, Schambach A, Moritz T, Cantz T, Thum T. miRNA screening reveals a new miRNA family stimulating iPS cell generation via regulation of Meox2. EMBO Rep. 2011 Oct 28;12(11):1153-9. doi:10.1038/embor.2011.176. PubMed PMID: 21941297.

Avedillo Díez I, Zychlinski D, Coci EG, Galla M, Modlich U, Dewey RA, Schwarzer A, Maetzig T, Mpofu N, Jaeckel E, Boztug K, Baum C, Klein C, Schambach A. Development of novel efficient SIN vectors with improved safety features for Wiskott-Aldrich syndrome stem cell based gene therapy. Mol Pharm. 2011 Oct 3;8(5):1525-37. Epub 2011 Aug 31. PubMed PMID: 21851067.

Rothe M, Rittelmeyer I, Iken M, Rüdrich U, Schambach A, Glage S, Manns MP, Baum C, Bock M, Ott M, Modlich U. Epidermal growth factor improves lentivirus vector gene transfer into primary mouse hepatocytes. Gene Ther. 2011 Aug 18. doi: 10.1038/gt.2011.117. [Epub ahead of print] PubMed PMID: 21850050.

Schott JW, Galla M, Godinho T, Baum C, Schambach A. Viral and Non-Viral Approaches for Transient Delivery of mRNA and Protein. Curr Gene Ther. 2011 Jul 25. [Epub ahead of print] PubMed PMID: 21787295.

Wu G, Liu N, Rittelmeyer I, Sharma AD, Sgodda M, Zaehres H, Bleidissel M, Greber B, Gentile L, Han DW, Rudolph C, Steinemann D, Schambach A, Ott M, Schöler HR, Cantz T. Generation of healthy mice from gene-corrected disease-specific induced pluripotent stem cells. PLoS Biol. 2011 Jul;9(7):e1001099. Epub 2011 Jul 12. PubMed PMID: 21765802; PubMed Central PMCID: PMC3134447.

van der Loo JC, Swaney WP, Grassman E, Terwilliger A, Higashimoto T, Schambach A, Baum C, Thrasher AJ, Williams DA, Nordling DL, Reeves L, Malik P. Scale-up and manufacturing of clinical-grade self-inactivating gamma-retroviral vectors by transient transfection. Gene Ther. 2011 Jul 14. doi:10.1038/gt.2011.102. [Epub ahead of print] PubMed PMID: 21753795.

Huston MW, van Til NP, Visser TP, Arshad S, Brugman MH, Cattoglio C, Nowrouzi A, Li Y, Schambach A, Schmidt M, Baum C, von Kalle C, Mavilio F, Zhang F, Blundell MP, Thrasher AJ, Verstegen MM, Wagemaker G. Correction of murine SCID-X1 by lentiviral gene therapy using a codon-optimized IL2RG gene and minimal pretransplant conditioning. Mol Ther. 2011 Oct;19(10):1867-77. doi:10.1038/mt.2011.127. Epub 2011 Jul 12. PubMed PMID: 21750532; PubMed Central PMCID: PMC3188737.

Pfaff N, Lachmann N, Kohlscheen S, Sgodda M, Araúzo-Bravo MJ, Greber B, Kues W, Glage S, Baum C, Niemann H, Schambach A, Cantz T, Moritz T. Efficient Hematopoietic Redifferentiation of Induced Pluripotent Stem Cells Derived from Primitive Murine Bone Marrow Cells. Stem Cells Dev. 2011 Aug 24. [Epub ahead of print] PubMed PMID: 21732815.

Pike-Overzet K, Rodijk M, Ng YY, Baert MR, Lagresle-Peyrou C, Schambach A, Zhang F, Hoeben RC, Hacein-Bey-Abina S, Lankester AC, Bredius RG, Driessen GJ, Thrasher AJ, Baum C, Cavazzana-Calvo M, van Dongen JJ, Staal FJ. Correction of murine Rag1 deficiency by self-inactivating lentiviral vector-mediated gene transfer. Leukemia. 2011 Sep;25(9):1471-83. doi: 10.1038/leu.2011.106. Epub 2011 May 27. PubMed PMID: 21617701.

Galla M, Schambach A, Falk CS, Maetzig T, Kuehle J, Lange K, Zychlinski D, Heinz N, Brugman MH, Göhring G, Izsvák Z, Ivics Z, Baum C. Avoiding cytotoxicity of transposases by dose-controlled mRNA delivery. Nucleic Acids Res. 2011 Sep 1;39(16):7147-60. Epub 2011 May 23. PubMed PMID: 21609958; PubMed Central PMCID: PMC3167617.

Zhang S, Chen S, Li W, Guo X, Zhao P, Xu J, Chen Y, Pan Q, Liu X, Zychlinski D, Lu H, Tortorella MD, Schambach A, Wang Y, Pei D, Esteban MA. Rescue of ATP7B function in hepatocyte-like cells from Wilson's disease induced pluripotent stem cells using gene therapy or the chaperone drug curcumin. Hum Mol Genet. 2011 Aug 15;20(16):3176-87. Epub 2011 May 18. PubMed PMID: 21593220.

Tiemann U, Sgodda M, Warlich E, Ballmaier M, Schöler HR, Schambach A, Cantz T. Optimal reprogramming factor stoichiometry increases colony numbers and affects molecular characteristics of murine induced pluripotent stem cells. Cytometry A. 2011 Jun;79(6):426-35. doi: 10.1002/cyto.a.21072. Epub 2011 May 4. PubMed PMID: 21548079.

Jacob R, Rüdrich U, Rothe M, Kirsch S, Maasoumy B, Narain N, Verfaillie CM, Sancho-Bru P, Iken M, Popescu I, Schambach A, Manns MP, Bock M. Induction of a mature hepatocyte phenotype in adult liver derived progenitor cells by ectopic expression of transcription factors. Stem Cell Res. 2011 May;6(3):251-61. Epub 2011 Feb 24. PubMed PMID: 21474405.

Baum C, Schambach A. Promoter competition for gene therapy of SCID-X1. Hum Gene Ther. 2011 Mar;22(3):255-6. PubMed PMID: 21401346.

Heckl D, Wicke DC, Brugman MH, Meyer J, Schambach A, Büsche G, Ballmaier M, Baum C, Modlich U. Lentiviral gene transfer regenerates hematopoietic stem cells in a mouse model for Mpl-deficient aplastic anemia. Blood. 2011 Apr 7;117(14):3737-47. Epub 2011 Feb 2. PubMed PMID: 21289307.

Warlich E, Kuehle J, Cantz T, Brugman MH, Maetzig T, Galla M, Filipczyk AA, Halle S, Klump H, Schöler HR, Baum C, Schroeder T, Schambach A. Lentiviral vector design and imaging approaches to visualize the early stages of cellular reprogramming. Mol Ther. 2011 Apr;19(4):782-9. Epub 2011 Feb 1. PubMed PMID: 21285961; PubMed Central PMCID: PMC3070104.

Bals C, Schambach A, Meyer J, Scheper T, Rinas U. Expression and purification of bioactive soluble murine stem cell factor from recombinant Escherichia coli using thioredoxin as fusion partner. J Biotechnol. 2011 Mar 10;152(1-2):1-8. Epub 2011 Jan 22. PubMed PMID: 21262286.

Maetzig T, Brugman MH, Bartels S, Heinz N, Kustikova OS, Modlich U, Li Z, Galla M, Schiedlmeier B, Schambach A, Baum C. Polyclonal fluctuation of lentiviral vector-transduced and expanded murine hematopoietic stem cells. Blood.
2011 Mar 17;117(11):3053-64. Epub 2011 Jan 19. PubMed PMID: 21248062.

Huelsmann PM, Hofmann AD, Knoepfel SA, Popp J, Rauch P, Di Giallonardo F, Danke C, Gueckel E, Schambach A, Wolff H, Metzner KJ, Berens C. A suicide gene approach using the human pro-apoptotic protein tBid inhibits HIV-1 replication. BMC Biotechnol. 2011 Jan 11;11:4. PubMed PMID: 21223573; PubMed Central PMCID:PMC3224247.

Heinz N, Schambach A, Galla M, Maetzig T, Baum C, Loew R, Schiedlmeier B. Retroviral and transposon-based tet-regulated all-in-one vectors with reduced background expression and improved dynamic range. Hum Gene Ther. 2011 Feb;22(2):166-76. Epub 2010 Dec 19. PubMed PMID: 20825282.

Turan S, Kuehle J, Schambach A, Baum C, Bode J. Multiplexing RMCE: versatile extensions of the Flp-recombinase-mediated cassette-exchange technology. J Mol Biol. 2010 Sep 10;402(1):52-69. Epub 2010 Jul 19. PubMed PMID: 20650281.

Ng YY, Baert MR, Pike-Overzet K, Rodijk M, Brugman MH, Schambach A, Baum C, Hendriks RW, van Dongen JJ, Staal FJ. Correction of B-cell development in Btk-deficient mice using lentiviral vectors with codon-optimized human BTK. Leukemia. 2010 Sep;24(9):1617-30. Epub 2010 Jun 24. PubMed PMID: 20574453.

Schambach A, Cantz T, Baum C, Cathomen T. Generation and genetic modification of induced pluripotent stem cells. Expert Opin Biol Ther. 2010 Jul;10(7):1089-103. Review. PubMed PMID: 20528610.

Suerth JD, Maetzig T, Galla M, Baum C, Schambach A. Self-inactivating alpharetroviral vectors with a split-packaging design. J Virol. 2010 Jul;84(13):6626-35. Epub 2010 Apr 21. PubMed PMID: 20410274; PubMed Central PMCID: PMC2903275.

Voelkel C, Galla M, Maetzig T, Warlich E, Kuehle J, Zychlinski D, Bode J, Cantz T, Schambach A, Baum C. Protein transduction from retroviral Gag precursors. Proc Natl Acad Sci U S A. 2010 Apr 27;107(17):7805-10. Epub 2010 Apr 12. PubMed PMID: 20385817; PubMed Central PMCID: PMC2867892.

Tomala M, Lavrentieva A, Moretti P, Rinas U, Kasper C, Stahl F, Schambach A,  Warlich E, Martin U, Cantz T, Scheper T. Preparation of bioactive soluble human leukemia inhibitory factor from recombinant Escherichia coli using thioredoxin as fusion partner. Protein Expr Purif. 2010 Sep;73(1):51-7. Epub 2010 Apr 8. PubMed PMID: 20381622.

Gama-Norton L, Herrmann S, Schucht R, Coroadinha AS, Löw R, Alves PM, Bartholomae CC, Schmidt M, Baum C, Schambach A, Hauser H, Wirth D. Retroviral vector performance in defined chromosomal Loci of modular packaging cell lines. Hum Gene Ther. 2010 Aug;21(8):979-91. PubMed PMID: 20222806.

Wurm M, Schambach A, Lindemann D, Hanenberg H, Ständker L, Forssmann WG, Blasczyk R, Horn PA. The influence of semen-derived enhancer of virus infection on the efficiency of retroviral gene transfer. J Gene Med. 2010 Feb;12(2):137-46. PubMed PMID: 20052742.

Pauwels K, Gijsbers R, Toelen J, Schambach A, Willard-Gallo K, Verheust C, Debyser Z, Herman P. State-of-the-art lentiviral vectors for research use: risk assessment and biosafety recommendations. Curr Gene Ther. 2009 Dec;9(6):459-74.Review. PubMed PMID: 20021330.

Broll S, Oumard A, Hahn K, Schambach A, Bode J. Minicircle performance depending on S/MAR-nuclear matrix interactions. J Mol Biol. 2010 Feb 5;395(5):950-65. Epub 2009 Dec 29. PubMed PMID: 20004666.

Cathomen T, Schambach A. Zinc-finger nucleases meet iPS cells: Zinc positive: tailored genome engineering meets reprogramming. Gene Ther. 2010 Jan;17(1):1-3. Epub 2009 Nov 12. PubMed PMID: 19907499.

Loew R, Meyer Y, Kuehlcke K, Gama-Norton L, Wirth D, Hauser H, Stein S, Grez M, Thornhill S, Thrasher A, Baum C, Schambach A. A new PG13-based packaging cell line for stable production of clinical-grade self-inactivating gamma-retroviral vectors using targeted integration. Gene Ther. 2010 Feb;17(2):272-80. Epub 2009 Oct 29. PubMed PMID: 19865181.

Maetzig T, Galla M, Brugman MH, Loew R, Baum C, Schambach A. Mechanisms controlling titer and expression of bidirectional lentiviral and gammaretroviral vectors. Gene Ther. 2010 Mar;17(3):400-11. Epub 2009 Oct 22. PubMed PMID: 19847204.

Metcalf JA, Ma X, Linders B, Wu S, Schambach A, Ohlemiller KK, Kovacs A, Bigg M, He L, Tollefsen DM, Ponder KP. A self-inactivating gamma-retroviral vector reduces manifestations of mucopolysaccharidosis I in mice. Mol Ther. 2010 Feb;18(2):334-42. Epub 2009 Oct 20. PubMed PMID: 19844196; PubMed Central PMCID: PMC2839301.

Modlich U, Navarro S, Zychlinski D, Maetzig T, Knoess S, Brugman MH, Schambach A, Charrier S, Galy A, Thrasher AJ, Bueren J, Baum C. Insertional transformation of hematopoietic cells by self-inactivating lentiviral and gammaretroviral vectors. Mol Ther. 2009 Nov;17(11):1919-28. Epub 2009 Aug 11. PubMed PMID: 19672245; PubMed Central PMCID: PMC2835038.

Maruggi G, Porcellini S, Facchini G, Perna SK, Cattoglio C, Sartori D, Ambrosi A, Schambach A, Baum C, Bonini C, Bovolenta C, Mavilio F, Recchia A. Transcriptional enhancers induce insertional gene deregulation independently from the vector type and design. Mol Ther. 2009 May;17(5):851-6. Epub 2009 Mar 17. PubMed PMID: 19293778; PubMed Central PMCID: PMC2835143.

Skokowa J, Lan D, Thakur BK, Wang F, Gupta K, Cario G, Brechlin AM, Schambach A, Hinrichsen L, Meyer G, Gaestel M, Stanulla M, Tong Q, Welte K. NAMPT is essential for the G-CSF-induced myeloid differentiation via a NAD(+)-sirtuin-1-dependent pathway. Nat Med. 2009 Feb;15(2):151-8. Epub 2009 Feb 1. PubMed PMID: 19182797.

Schambach A, Swaney WP, van der Loo JC. Design and production of retro- and lentiviral vectors for gene expression in hematopoietic cells. Methods Mol Biol. 2009;506:191-205. PubMed PMID: 19110628.

Modlich U, Schambach A, Li Z, Schiedlmeier B. Murine hematopoietic stem cell transduction using retroviral vectors. Methods Mol Biol. 2009;506:23-31. PubMed PMID: 19110617.

Schambach A, Baum C. Clinical application of lentiviral vectors - concepts and practice. Curr Gene Ther. 2008 Dec;8(6):474-82. Review. PubMed PMID: 19075630.

Cornils K, Lange C, Schambach A, Brugman MH, Nowak R, Lioznov M, Baum C, Fehse B. Stem cell marking with promotor-deprived self-inactivating retroviral vectors does not lead to induced clonal imbalance. Mol Ther. 2009 Jan;17(1):131-43. Epub 2008 Nov 11. PubMed PMID: 19002163; PubMed Central PMCID: PMC2834973.

Moreno-Carranza B, Gentsch M, Stein S, Schambach A, Santilli G, Rudolf E, Ryser MF, Haria S, Thrasher AJ, Baum C, Brenner S, Grez M. Transgene optimization significantly improves SIN vector titers, gp91phox expression and reconstitution of superoxide production in X-CGD cells. Gene Ther. 2009 Jan;16(1):111-8. Epub 2008 Sep 11. PubMed PMID: 18784749.

Milsom MD, Jerabek-Willemsen M, Harris CE, Schambach A, Broun E, Bailey J, Jansen M, Schleimer D, Nattamai K, Wilhelm J, Watson A, Geiger H, Margison GP, Moritz T, Baum C, Thomale J, Williams DA. Reciprocal relationship between O6-methylguanine-DNA methyltransferase P140K expression level and chemoprotection of hematopoietic stem cells. Cancer Res. 2008 Aug 1;68(15):6171-80. PubMed PMID: 18676840.

Sharma AD, Cantz T, Vogel A, Schambach A, Haridass D, Iken M, Bleidissel M, Manns MP, Schöler HR, Ott M. Murine embryonic stem cell-derived hepatic progenitor cells engraft in recipient livers with limited capacity of liver tissue formation. Cell Transplant. 2008;17(3):313-23. PubMed PMID: 18522234.

Modlich U, Schambach A, Brugman MH, Wicke DC, Knoess S, Li Z, Maetzig T, Rudolph C, Schlegelberger B, Baum C. Leukemia induction after a single retroviral vector insertion in Evi1 or Prdm16. Leukemia. 2008 Aug;22(8):1519-28. Epub 2008 May 22. PubMed PMID: 18496560.

Müller LU, Milsom MD, Kim MO, Schambach A, Schuesler T, Williams DA. Rapid lentiviral transduction preserves the engraftment potential of Fanca(-/-) hematopoietic stem cells. Mol Ther. 2008 Jun;16(6):1154-60. Epub 2008 Apr 8. PubMed PMID: 18398427.

Zychlinski D, Schambach A, Modlich U, Maetzig T, Meyer J, Grassman E, Mishra A, Baum C. Physiological promoters reduce the genotoxic risk of integrating gene vectors. Mol Ther. 2008 Apr;16(4):718-25. Epub 2008 Mar 4. PubMed PMID: 18334985.

Galla M, Schambach A, Towers GJ, Baum C. Cellular restriction of retrovirus particle-mediated mRNA transfer. J Virol. 2008 Mar;82(6):3069-77. Epub 2008 Jan 16. PubMed PMID: 18199655; PubMed Central PMCID: PMC2258976.

Thornhill SI, Schambach A, Howe SJ, Ulaganathan M, Grassman E, Williams D, Schiedlmeier B, Sebire NJ, Gaspar HB, Kinnon C, Baum C, Thrasher AJ. Self-inactivating gammaretroviral vectors for gene therapy of X-linked severe combined immunodeficiency. Mol Ther. 2008 Mar;16(3):590-8. Epub 2008 Jan 8. PubMed PMID: 18180772.

Baum C, Schambach A, Modlich U, Thrasher A. [Gene therapy of SCID-X1]. Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. 2007 Dec;50(12):1507-17. Review. German. PubMed PMID: 18046520.

Heuser M, Argiropoulos B, Kuchenbauer F, Yung E, Piper J, Fung S, Schlenk RF, Dohner K, Hinrichsen T, Rudolph C, Schambach A, Baum C, Schlegelberger B, Dohner H, Ganser A, Humphries RK. MN1 overexpression induces acute myeloid leukemia in mice and predicts ATRA resistance in patients with AML. Blood. 2007 Sep 1;110(5):1639-47. Epub 2007 May 9. PubMed PMID: 17494859.

Schambach A, Baum C. Vector design for expression of O6-methylguanine-DNA methyltransferase in hematopoietic cells. DNA Repair (Amst). 2007 Aug 1;6(8):1187-96. Epub 2007 May 7. Review. PubMed PMID: 17482894; PubMed Central PMCID: PMC2128767.

Zhang F, Thornhill SI, Howe SJ, Ulaganathan M, Schambach A, Sinclair J, Kinnon C, Gaspar HB, Antoniou M, Thrasher AJ. Lentiviral vectors containing an enhancer-less ubiquitously acting chromatin opening element (UCOE) provide highly reproducible and stable transgene expression in hematopoietic cells. Blood. 2007 Sep 1;110(5):1448-57. Epub 2007 Apr 24. PubMed PMID: 17456723; PubMed Central PMCID: PMC2629730.

Schambach A, Galla M, Maetzig T, Loew R, Baum C. Improving transcriptional termination of self-inactivating gamma-retroviral and lentiviral vectors. Mol Ther. 2007 Jun;15(6):1167-73. Epub 2007 Apr 3. PubMed PMID: 17406345.

Unit 14 - RG Lymphatic Cell Therapy

Salguero G, Sundarasetty BS, Borchers S, Wedekind D, Eiz-Vesper B, Velaga S, Jirmo AC, Behrens G, Warnecke G, Knöfel AK, Blasczyk R, Mischak-Weissinger E, Ganser A, Stripecke R. Preconditioning Therapy with Lentiviral Vector-Programmed Dendritic Cells Accelerates the Homeostatic Expansion of Antigen-Reactive Human T Cells in NOD.Rag1(-/-).IL-2r?c(-/-) mice. Hum Gene Ther. 2011 Jul 27. [Epub ahead of print]

Rickmann M, Krauter J, Stamer K, Heuser M, Salguero G, Mischak-Weissinger E, Ganser A, Stripecke R. (2011)   Elevated frequencies of leukemic myeloid and plasmacytoid dendritic cells in acute myeloid leukemia with the FLT3 internal tandem duplication. Ann Hematol.;90(9):1047-58.

Pincha M, Salguero G, Wedekind D, Sundarasetty BS, Lin A, Kasahara N, Brugman MH, Jirmo AC, Modlich U, Gutzmer R, Büsche G, Ganser A, Stripecke R.(2011) Lentiviral vectors for induction of self-differentiation and conditional ablation of dendritic cells. Gene Ther.;18(8):750-64.

Pincha M , Sundarasetty BS and Stripecke R (2010). Lentiviral Vectors for Immunization: an Inflammatory Field. Expert Reviews Vaccines; (3):309-21.

Jirmo AC, Koya RC, Sundarassety BS, Pincha M, Yu G-Y, Lai M, Bakshi R, Schlaphoff V, Grabowski J, Behrens G, Wedemeyer H, Stripecke R  (2010). Monocytes Transduced with Lentiviral Vectors Expressing Hepatitis C Virus Non-Structural Proteins and Differentiated into Dendritic Cells Stimulate Multi-antigenic CD8+ T cell responses. Vaccine 28(4):922-33.

Stripecke R. (2009). Lentiviral vector-mediated genetic programming of mouse and human dendritic cells. Methods Mol Biol.; 506:139-58.

Koya RC, Weber JS, Kasahara N, Lau R, Levine AM, Stripecke R. Making Dendritic Cells from Inside-Out: lentiviral vector-mediated gene delivery of GM-CSF and IL-4 into CD14+ monocytes generates DCs in vitro. Human Gene Therapy. 2004; 15:733-48.

Koya R, Kimura T, Ribas A, Rozengurt N, Lawson GW, Faure-Kumar E, Wang H-J, Herschmann H, Kasahara K, Stripecke R. Lentiviral vector-mediated autonomous differentiation of mouse bone marrow cells into immunologically potent dendritic cell vaccines. Mol Ther. 2007; 15(5):971-80.

Kimura T, Koya RC, Anselmi L, Sternini C, Wang H, Comin-Anduix B, Prins R, Faure-Kumar E, Cui Y, Rozengurt N, Kasahara N, Stripecke R. Lentiviral Vectors with CMV or MHCII Promoters administered in vivo: Immune reactivity versus persistence of expression. Mol Ther. 2007; 15(7):1390-9.

Köchling J, Prada J, Bahrami M, Stripecke R, Seeger K, Henze G, Wittig B, Schmidt M. Anti-tumor effect of DNA-based vaccination and dSLIM immunomodulatory molecules in mice with Ph(+) acute lymphoblastic leukaemia. Vaccine. 2008; 26;26(36):4669-75.

Stripecke R. Lentiviral vector-mediated genetic programming of mouse and human dendritic cells. Methods Mol. Biol. 2009; 506:139-58.

Sarafian T, Montes C, Harui A, Beedanagari SR, Kiertscher S, Stripecke R, Hossepian D, Kitchen C, Kern R, Belperio J, Roth MD (2008). Clarifying CB2 receptor-dependent and independent effects of THC on human lung epithelial cells. Toxicol Appl Pharmacol. 15;231(3):282-90.

Cheng JC, Kinjo K, Judelson DR, Chang J, Wu WS, Schmid I, Shankar DB, Kasahara N, Stripecke R, Bhatia R, Landaw EM, Sakamoto KM (2008). CREB is a critical regulator of normal hematopoiesis and leukemogenesis. Blood 111(3):1182-92.

Unit 15 - JRG Lymphocyte Biology

Zietara, N., Lyszkiewicz, M., Krueger, A., and Weiss, S. (2011). ICOS-dependent stimulation of NKT cells by marginal zone B cells. Eur J Immunol, doi: 10.1002/eji.201041092

Krueger, A. (2011). A missing link in thymic dendritic cell development. Eur J Immunol, 41, 2145-7.

Haas, J. D., Nistala, K., Petermann, F., Saran, N., Chennupati, V., Schmitz, S., Korn, T., Wedderburn, L. R., Förster, R., Krueger, A.*, and Prinz, I*. (2011). Expression of miRNAs miR-133b and miR-206 in the Il17a/f locus is co-regulated with IL-17 production in ab and yd T cells. PLoS One, 6(5), e20171. (* Equal contribution).

Surdziel, E., Cabanski, M., Dallmann, I., Lyszkiewicz, M., Krueger, A., Ganser, A., Scherr, M., and Eder, M. (2011). Enforced expression of miR-125b affects myelopoiesis by targeting multiple signalling pathways. Blood 117, 4338-48.

Lyszkiewicz, M., Witzlau, K., Pommerencke, J., and Krueger, A. (2011). Chemokine receptor CX3CR1 promotes dendritic cell development under steady-state conditions. Eur J Immunol, 41, 1256-65.

Krueger A*, Willenzon S, Lyszkiewicz M, Kremmer E, Förster R. CC chemokine receptor (CCR) 7 and 9 double-deficient hematopoietic progenitors are severely impaired in seeding the adult thymus. Blood. 2010; 115: 1906-12 * Corresponding author.

Saran N, Lyszkiewicz M, Pommerencke J, Witzlau K, Vakilzadeh R, Ballmaier, M, von Boehmer H, Krueger A. Multiple extra-thymic precursors contribute to T cell development with different kinetics. Blood. 2010; 115: 1137-44.

Weber C*, Krueger A*, Münk A, Bode C, Van Veldhoven PP, Gräler MH. Discontinued Postnatal Thymocyte Development in Sphingosine 1-Phosphate-Lyase-Deficient Mice. J Immunol. 2009; 183: 4292-301. * Equal contribution.

Garbe AI*, Krueger A*, Gounari F, Zuniga-Pflucker JC, von Boehmer H. (2006) Differential synergy of Notch and T cell receptor signaling determines lineage fate in lymphocyte development. J Exp Med 203, 1579-90. (* Equal contribution).

Krueger A, von Boehmer H. Identification of a T lineage committed progenitor in adult blood. Immunity. 2007; 26, 105-116.

Junt T, Schulze H, Chen Z, Massberg S, Goerge T, Krueger A, Wagner D, Graf T, Italiano Jr, JE, Shivdasani RA, von Andrian UH. Dynamic visualization of thrombopoiesis within bone marrow. Science. 2007; 317, 1767-70.

Kreslavsky T, Garbe AI, Krueger A, von Boehmer H. T-cell receptor instructed alphabeta versus gammadelta lineage commitment. J Exp Med. 2008; 205, 1173-86.

Krueger A*, Garbe AI, von Boehmer H. Phenotypic plasticity of T cell progenitors upon exposure to Notch ligands. J Exp Med. 2006; 203: 1977-84. * Corresponding author.

Unit 16 - JRG Hepatic Cell Therapy

Rothe M, Rittelmeyer I, Iken M, Rüdrich U, Schambach A, Glage S, Manns MP , Baum C, Bock M, Ott M, Modlich U. Epidermal growth factor improves lentivirus vector gene transfer into primary mouse hepatocytes. Gene Therapy 2011: in press.

Iacob R, Rüdrich U, Rothe M, Kirsch S, Maasoumy B, Narain N, Verfaillie CM, Sancho-Bru P, Iken M, Popescu I, Schambach A, Manns MP, Bock M. Induction of a mature hepatocyte phenotype in adult liver derived progenitor cells by ectopic expression of transcription factors. Stem Cell Res 2011;6:251-61

Haridass D, Yuan Q, Becker PD, Cantz T, Iken M, Narain N, Bock M, Nörder M, Legrand N, Wedemeyer H, Weijer K, Spits H, Manns MP, Cai J, Deng H, Di Santo JP, Guzman CA, Ott M. Adult hepatocytes repopulate the liver more efficiently than fetal liver progenitors and embryonic stem cell hepatic precursors in immunodeficient Alb-uPA transgenic mice. Am J Pathol 2009;175(4):1483-92.

Meyburg J, Das AM, Hoerster F, Lindner M, Kriegbaum H, Engelmann G, Schmidt J, Ott M, Pettenazzo A, Luecke T, Bertram H, Hoffmann G, Burlina A. One liver for four children: First clinical series of liver cell transplantation for severe neonatal urea cycle defects. Transplantation. 2009; in press.

Sancho-Bru P, Najimi M, Caruso M, Pawelyn K, Cantz T, Forbes SJ, Roskams T, Ott M, Gehling U, Sokal E, Verfaillie C, Muraca M. Stem and progenitor cells for liver repopulation: can we standardize the process from bench to bedside? Gut. 2008; Dec 17. [Epub ahead of print].

Sharma AD, Cantz T, Vogel A, Schambach A, Haridass D, Iken M, Bleidissel M, Manns MP, Schöler HR, Ott M. Murine embryonic stem cell-derived hepatic progenitor cells engraft in recipient livers with limited capacity of liver tissue formation. Cell Transplant. 2008;17(3):313-23.

Jochheim-Richter A, Rüdrich U, Koczan D, Hillemann T, Tewes S, Petry M, Kispert A, Sharma AD, Attaran F, Manns MP, Ott M. Gene expression analysis identifies novel genes participating in early murine liver development and adult liver regeneration. Differentiation. 2006; Apr;74(4):167-73.

Unit 17 - JRG Tolerance

A. Bajor, S. Tischer, C. Figueiredo, M. Wittmann, S. Immenschuh, R. Blasczyk, B. Eiz-Vesper, Modulatory role of calreticulin as chaperokine for dendritic cell-based immunotherapy, Clin Exp Immunol, 165 (2011) 220-234.

Y. Jaimes, A. Seltsam, B. Eiz-Vesper, R. Blasczyk, C. Figueiredo, Regulation of HLA class II expression prevents allogeneic T-cell responses, Tissue Antigens, 77 (2011) 36-44.

J. Martens, M. Verboom, C. Figueiredo, B. Eiz-Vesper, R. Blasczyk, S. Immenschuh, The new HLA allele, HLA-A*03:57, differs from HLA-A*03:01 by two amino acids at positions 76 and 77 in the alpha2 domain affecting the pocket F of the peptide-binding groove, Tissue Antigens.

L. Schlahsa, Y. Jaimes, R. Blasczyk, C. Figueiredo, Granulocyte-colony-stimulatory factor: a strong inhibitor of natural killer cell function, Transfusion, 51 (2011) 293-305.

C. Figueiredo, L. Goudeva, P.A. Horn, B. Eiz-Vesper, R. Blasczyk, A. Seltsam, Generation of HLA-deficient platelets from hematopoietic progenitor cells, Transfusion, 50 (2010) 1690-1701.

Y. Jaimes, C. Figueiredo, R. Blasczyk, Silencing the expression of platelet endothelial cell adhesion molecule-1 prevents allogeneic T-cell cytotoxicity, Transfusion, 50 (2010) 1988-2000.

C. Figueiredo, Y. Jaimes, P.A. Horn, R. Blasczyk, B. Eiz-Vesper, The new HLA-Cw*0524 allele increase the variability of position 49 in HLA-C, Tissue Antigens, 74 (2009) 258-259.

C. Figueiredo, S. Mbulaiteye, C.M. Ndugwa, T. Schulz, B. Eiz-Vesper, Assessment of the amino acid substitution in HLA-B*5719 for allorecognition, Tissue Antigens, 73 (2009) 376-378.

C. Figueiredo, A. Seltsam, R. Blasczyk, Permanent silencing of NKG2A expression for cell-based therapeutics, J Mol Med (Berl), 87 (2009) 199-210.

C. Figueiredo, M. Wittmann, D. Wang, R. Dressel, A. Seltsam, R. Blasczyk, B. Eiz-Vesper, Heat shock protein 70 (HSP70) induces cytotoxicity of T-helper cells, Blood, 113 (2009) 3008-3016.

C. Gras, S. Immenschuh, B. Eiz-Vesper, R. Blasczyk, C. Figueiredo, The novel allele HLA-B*35:167 differs from HLA-B*35:03:01 by the amino acid exchange Val152Glu, Tissue Antigens. 2011 Nov 15. doi: 10.1111/j.1399-0039.2011.01802.x. [Epub ahead of print]

M. Guech-Ongey, M. Verboom, R.M. Pfeiffer, T.F. Schulz, C.M. Ndugwa, A.M. Owor, P.M. Bakaki, K. Bhatia, C. Figueiredo, B. Eiz-Vesper, R. Blasczyk, S.M. Mbulaiteye, HLA polymorphisms and detection of kaposi sarcoma-associated herpesvirus DNA in saliva and peripheral blood among children and their mothers in the uganda sickle cell anemia KSHV Study, Infect Agent Cancer, 5 21.

J. Hinrichs, C. Figueiredo, K. Hirv, J. Mytilineos, R. Blasczyk, P.A. Horn, B. Eiz-Vesper, Discrimination of HLA null and low expression alleles by cytokine-induced secretion of recombinant soluble HLA, Mol Immunol, 46 (2009) 1451-1457.

A.C. Jirmo, C.H. Nagel, C. Bohnen, B. Sodeik, G.M.N. Behrens, Contribution of direct and cross presentation to CTL immunity against herpes simplex virus 1. J Immunol. 2009;182:283-92.

H. Constable, M. Stankov, C. Hartwig, T. Tschernig, G.M.N. Behrens, Impaired lung dendritic cell migration and T cell stimulation induced by immunostimulatory oligonucleotides contribute to reduced allergic airway inflammation. J Immunol. 2009;183:3443-53.

C. Figueiredo, P.A. Horn, R. Blasczyk, A. Seltsam, Regulating MHC expression for cellular therapeutics. Transfusion. 2007 Jan;47(1):18-27.

E. Jaeckel, N. Mpofu, N. Saal, M.P. Manns, Role of regulatory T cells for the treatment of type 1 diabetes mellitus. Horm Metab Res. 2008; 40:126-36.

C. Figueiredo, M. Wittmann, R. Dressel, A. Seltsam, R. Blasczyk, B. Eiz-Vesper, Heat Shock Protein 70 (HSP70) induces cytotoxicity of T helper cells. Blood. 2008 Nov 18. [Epub ahead of print]

M. Stankov, R.E. Schmidt, G.M.N. Behrens, Zidovudine impairs adipogenic differentiation through inhibition of clonal expansion. Antimicrob AGents Chemother 2008; 52:2882-9.

N. Mpofu, M. Hardtke-Wolenski, N. Saal, M.P. Manns, E. Jaeckel, Antigen-specific Tregs prevent T1D by long lasting persistence and infectious tolerance. (submitted)

C.K. Prajeeth, T. Ebensen, C.A. Guzmann, S. Weiss, R.E. Schmidt, H. Constabel, G.M.N. Behrens, Distinct effects of the synthetic TLR2/6 agonist BPPcysMPEG on cross-priming and antigen presentation by dendritic cells. (submitted)

A.M. Baru, A. Rathinasamy, A.C. Jirmo, M. Scherr, M. Eder, G.M.N. Behrens, Dendritic cells derived from HOXB4-immortalized hematopoietic bone marrow cells. (submitted)

Unit 18 - W3 Cardiorespiratory Tissue Engineering

Fiedler J, Jazbutyte V, Kirchmaier BC, Gupta SK, Lorenzen J, Hartmann D, Galuppo P, Kneitz S, Pena JT. Sohn-Lee C, Loyer X, Soutschek J, Brand T, Tuschl T, Heineke J, Martin U, Schulte-Merker S, Ertl G, Engelhardt S, Bauersachs J, Thum T: MicroRNA-24 Regulates Vascularity After Myocardial Infarction. Circulation 2011; 124 no. 6:720-30.

Hilfiker A, Kasper C, Hass R, Haverich A: Mesenchymal stem cells and progenitor cells in connective tissue engineering and regenerative medicine: is there a future for transplantation? Langenbecks Arch Surg. 2011 Apr;396(4):489-97. Epub 2011 Mar 4. Review.

Hoch M, Fischer P, Stapel B, Missol-Kolka E, Sekkali B, Scherr M, Favret F, Braun T, Eder M, Schuster-Gossler K, Gossler A, Hilfiker A, Balligand J L, Drexler H, Hilfiker-Kleiner D: Erythropoietin Preserves the Endothelial Differentiation Capacity of Cardiac Progenitor Cells and Reduces Heart Failure during Anticancer Therapies. Cell Stem Cell. 2011 Aug 5;9(2):131-43.

Kensah G*, Gruh I*, Viering J, Schumann H, Dahlmann J, Meyer H, Skvorc D, Bär A, Akhyari P, Heisterkamp A, Haverich A, Martin U: A novel miniaturized multimodal bioreactor for continuous in situ assessment of bioartificial cardiac tissue during stimulation and maturation. Tissue Eng Part C 2011; 17 no. 4:463-73. *Authors contributed equally.

Martens A*, Gruh I*, Dimitroulis D, Rojas SV, Schmidt-Richter I, Rathert C, Khaladj N, Gawol A, Chikobava MG, Martin U, Haverich A, Kutschka I.: Rhesus Monkey Cardiosphere Derived Cells for Myocardial Restoration.Cytotherapy 2011; 13 no. 7:864-72. *Authors contributed equally.

Martin U: iPS cells from blood. In: Regenerative Therapy Using Blood Derived Stem Cells. In press.

Mauritz C*, Martens A*, V. Rojas S, Schnick T, Rathert C, Schecker N, Menke S, Glage S, Zweigerdt R, Haverich A, Martin U#, Kutschka I#: Induced pluripotent stem cell (iPSC)-derived Flk-1 progenitor cells engraft, differentiate, and improve heart function in a mouse model of acute myocardial infarction. European Heart Journal. [Published online May 19, 2011]. DOI: 10.1093/eurheartj/ehr166. * #Authors contributed equally. 

Paleek J, Dräger G, Zweigert R, Olmer R, Martin U, Kirschning A: A practical synthesis of Rho-Kinase inhibitor Y-27632 and fluoro derivatives and their evaluation in human pluripotent stem cells. Organ Biomol Chem 2011; 9, no.15:5503-10.

Zweigerdt R*, Olmer R*, Singh H, Haverich A, Martin U: Scalable expansion of human pluripotent stem cells in suspension culture. Nat Protoc 2011; 6, no. 5:689. *Authors contributed equally.

Cantz T and Martin U. Induced Pluripotent Stem (iPS) Cells: Characteristics and Perspectives. Adv Biochem Eng Biotechnol, 2010.

Bär A, Dorfman SE, Fischer P, Hilfiker-Kleiner D, Cebotari S, Tudorache I, Suprunov M, Haverich A, and Hilfiker A: The pro-angiogenic factor CCN1 enhances the re-endothelialization of biological vascularized matrices in vitro. Cardiovasc Res 2010; 85, no. 4:806.

Cebotari S, Tudorache I, Jaekel T, Hilfiker A, Dorfman S, Ternes W, Haverich A, Lichtenberg A: Detergent decellularization of heart valves for tissue engineering: toxicological effects of residual detergents on human endothelial cells. Artif Organs 2010; 34, no. 3:206-10.

Hata A, Bär A., Dorfman SE, Vukadinovic Z, Sawa Y, Haverich A and Hilfiker A. Engineering a novel three-dimensional contractile myocardial patch with cell sheets and decellularised matrix. Eur J Cardiothorac Surg. 2010.

Hess C, Wiegmann B, Maurer AN, Fischer P, Möller L, Martin U, Hilfiker A, Haverich A, Fischer S: Reduced thrombocyte adhesion to endothelialized poly 4-methyl-1-pentene gas exchange membranes - a first step toward bioartificial lung development. Tissue Eng Part A 2010; 16, no. 10:3043-53.

Hilfiker-Kleiner D, Shukla P, Klein G, Schaefer A, Stapel B, Hoch M, Muller W, Scherr M, Theilmeier G, Ernst M, Hilfiker A, and Drexler H: Continuous glycoprotein-130-mediated signal transducer and activator of transcription-3 activation promotes inflammation, left ventricular rupture, and adverse outcome in subacute myocardial infarction. Circulation 2010; 122, no. 2:145.

Kühn C, Graf K, Heuer W, Hilfiker A, Chaberny IF, Stiesch M, and Haverich A: Economic implications of infections of implantable cardiac devices in a single institution. Eur J Cardiothorac Surg 2010; 37 no. 4:875.

Kühn C, Graf K, Mashaqi B, Pichlmaier M, Heuer W, Hilfiker A, Stiesch M, Chaberny IF, and Haverich A: Prevention of early vascular graft infection using regional antibiotic release. J Surg Res 2010; 164, no. 1:e185.

Olmer R, Haase A, Merkert S, Cui W, Palecek J, Kirschning A, Glage S, Miller K, Martin U. Long term expansion of undifferentiated human iPS cells in suspension culture using a defined medium. Stem Cell Research 2010; 5, no. 1:51-64.

Ozkan J, and Martin U: In vitro generation of large blood vessels is the key technology for the generation and implantation of larger dimension tissue. Circulation 2010; 121, no. 13:F76.

Mauritz C and Martin U. 2010. Embryonic Stem Cells: Differentiation into Respiratory Cell Derivatives. In Stem cells: organogenesis and cancer, S.R. Singh, editor. Research Signpost 2010.

Singh H, Mok P, Balakrishnan T, Rahmat SN, Zweigerdt R: Up-scaling single cell-inoculated suspension culture of human embryonic stem cells. Stem Cell Res 2010; 3:165-79.

Tomala M, Lavrentieva A, Moretti P, Rinas U, Stahl F, Wahrlich E, Martin U, Cantz T, and Scheper T. 2010. Preparation of Bioactive Soluble Human Leukemia Inhibitory Factor from recombinant Escherichia coli using Thioredoxin as Fusion Partner. Protein Expression and Purification, 2010

Wernet P, Trapp T, Zweigerdt R, Mann J, Trompeter HI: Lentiviral labeling reveals three germ layer differentiation potential of a single unrestricted somatic stem cell from human cord blood. Exp Hematol 2010; 38, no. 11:1099-104.

Baraki H, Tudorache I, Braun M, Hoffler K, Gorler A, Lichtenberg A, Bara C, Calistru A, Brandes G, Hewicker-Trautwein M, Hilfiker A, Haverich A, and Cebotari S: Orthotopic replacement of the aortic valve with decellularized allograft in a sheep model. Biomaterials 2009; 30, no. 31:6240.

Gruh I, Martin U. Transdifferentiation of stem cells: A critical view. Adv Biochem Eng Biotechnol 2009; 114:73-106.

Haase A, Olmer R, Schwanke K, Wunderlich S, Merkert S, Hess C, Zweigerdt R, Gruh I, Meyer J, Wagner S, Maier LS, Han DW, Glage S, Miller K, Fischer P, Schöler HR, Martin U. Generation of induced pluripotent stem (iPS) cells from human cord blood. Cell Stem Cell 2009, 5, no. 4:434-41.

Tudorache I, Kostin S, Meyer T, Teebken O, Bara C, Hilfiker A, Haverich A, Cebotari S: Viable vascularised autologous patch for transmural myocardial reconstruction. Eur J Cardiothorac Surg 2009;36, no. 2:306-11; discussion 311.

Xaymardan M, Cimini M, Fazel S, Weisel RD, Lu WY, Martin U, Harvey RP, and Li RK. 2009. c-Kit Function Is Necessary for In Vitro Myogenic Differentiation of Bone Marrow Hematopoietic Cells. Stem Cells 27, no. 8:1911.

Ghodsizad A, Niehaus M, Koegler G, Martin U, Wernet P, Bara C, Khaladj N, Loos A., Makoui M, Thiele J, Mengel M, Karck M, Klein HM, Haverich A, Ruhparwar A. 2008. Transplanted human cord blood derived unrestricted somatic stem cells improve left-ventricular function and prevent left-ventricular dilation and scar formation after acute myocardial infarction. Heart 2009; 95, no. 1:27-35.

Gruh I, Wunderlich S, Winkler M, Schwanke K, Heinke J, Blomer U, Ruhparwar A, Rohde B, Li RK, Haverich A, Martin U. Human CMV immediate-early enhancer: a useful tool to enhance cell-type-specific expression from lentiviral vectors. J Gene Med. 2008; 10, 1-21.

Martin U. 2008. Methods for studying stem cells: Adult stem cells for lung repair. Methods 45, no. 2:121.

Mauritz C, Schwanke K, Reppel M, Neef S, Katsirntaki K, Maier LS, Nguemo F, Menke S, Haustein M, Hescheler J, Hasenfuss G, Martin U. Generation of functional murine cardiac myocytes from induced pluripotent stem cells. Circulation. 2008;118, 5-507.

Winkler ME, Mauritz C, Groos S, Kispert A, Menke S, Hoffmann A, Gruh I, Schwanke K, Haverich A, Martin U. Serum-Free Differentiation of Murine Embryonic Stem Cells into Alveolar Type II Epithelial Cells. Cloning Stem Cells. 2008;10, 1-49.

Wunderlich S, Gruh I, Winkler ME, Beier J, Radtke K, Schmiedl A, Groos S, Haverich A, Martin U. Type II pneumocyte-restricted green fluorescent protein expression after lentiviral transduction of lung epithelial cells. Hum Gene Ther. 2008; 19, 1-39.

Aper T, Schmidt A, Duchrow M, Bruch HP. 2007. Autologous blood vessels engineered from peripheral blood sample. Eur J Vasc Endovasc Surg 33, no. 1:33.

Bar A, Haverich A, Hilfiker A. 2007. Cardiac tissue engineering: "reconstructing the motor of life". Scand J Surg 96, no. 2:154.

Hilfiker-Kleiner D, Kaminski K, Podewski E, Bonda T, Schaefer A, Sliwa K, Forster O, Quint A, Landmesser U, Doerries C, Luchtefeld M, Poli V, Schneider MD, Balligand JL, Desjardins F, Ansari A, Struman I, Nguyen NQ, Zschemisch NH, Klein G, Heusch G, Schulz R, Hilfiker A, Drexler H. 2007. A cathepsin D-cleaved 16 kDa form of prolactin mediates postpartum cardiomyopathy. Cell 128, no. 3:589.

Lichtenberg A, Cebotari S, Tudorache I, Hilfiker A, Haverich A. 2007. Biological scaffolds for heart valve tissue engineering. Methods Mol Med 140:309.

Ruhparwar A, Er F, Martin U, Radke K, Gruh I, Niehaus M, Karck M, Haverich A, Hoppe UC. 2007. Enrichment of cardiac pacemaker-like cells: neuregulin-1 and cyclic AMP increase I(f)-current density and connexin 40 mRNA levels in fetal cardiomyocytes.Med Biol Eng Comput 45, no. 2:221.

Tudorache I, Cebotari S, Sturz G, Kirsch L, Hurschler C, Hilfiker A., Haverich A, Lichtenberg A. 2007. Tissue engineering of heart valves: biomechanical and morphological properties of decellularized heart valves. J Heart Valve Dis 16, no. 5:567.

Unit 19 - JRG Myocardial Tissue Engineering

 G. Kensah, I. Gruh, J. Viering, H. Schumann, J. Dahlmann, H. Meyer, D. Skvorc, A. Bar, P. Akhyari, A. Heisterkamp, A. Haverich, U. Martin, A novel miniaturized multimodal bioreactor for continuous in situ assessment of bioartificial cardiac tissue during stimulation and maturation, Tissue Eng Part C Methods, 17 (2011) 463-473.

K. Kütemeyer, G. Kensah, M. Heidrich, H. Meyer, U. Martin, I. Gruh, A. Heisterkamp, Two-photon induced collagen cross-linking in bioartificial cardiac tissue, Optics Express, 19 (2011) 15996-16007.

A. Martens, I. Gruh, D. Dimitroulis, S.V. Rojas, I. Schmidt-Richter, C. Rathert, N. Khaladj, A. Gawol, M.G. Chikobava, U. Martin, A. Haverich, I. Kutschka, Rhesus monkey cardiosphere-derived cells for myocardial restoration, Cytotherapy, 13 (2011) 864-872.

L. Moller, A. Krause, J. Dahlmann, I. Gruh, A. Kirschning, G. Drager, Preparation and evaluation of hydrogel-composites from methacrylated hyaluronic acid, alginate, and gelatin for tissue engineering, The International journal of artificial organs, 34 (2011) 93-102.

I. Gruh, U. Martin, 2009. Transdifferentiation of stem cells: a critical view. Adv Biochem Eng Biotechnol 114:73-106.

A. Haase, R. Olmer, K. Schwanke, S. Wunderlich, S. Merkert, C. Hess, R. Zweigerdt, I. Gruh, J. Meyer, S. Wagner, L.S. Maier, D.W. Han, S. Glage, K. Miller, P. Fischer, H.R. Scholer, and U. Martin. 2009. Generation of induced pluripotent stem cells from human cord blood. Cell Stem Cell 5:434-441.

I. Gruh, S. Wunderlich, M. Winkler, K. Schwanke, J. Heinke, U. Blomer, A. Ruhparwar, B. Rohde, R.K. Li, A. Haverich, U. Martin, Human CMV immediate-early enhancer: a useful tool to enhance cell-type-specific expression from lentiviral vectors. J Gene Med. (2008); 10, 21-32.

S. Wunderlich, I. Gruh, M.E. Winkler, J. Beier, K. Radtke, A. Schmiedl, S. Groos, A. Haverich, U. Martin, Type II pneumocyte-restricted green fluorescent protein expression after lentiviral transduction of lung epithelial cells. Hum Gene Ther. 2008; 19, 39-52.

M.E. Winkler, C. Mauritz, S. Groos, A. Kispert, S. Menke, A. Hoffmann, I. Gruh, K. Schwanke, A. Haverich, U. Martin, Serum-free differentiation of murine embryonic stem cells into alveolar type II epithelial cells. Cloning Stem Cells. (2008); 10, 49-64.

I. Gruh, J. Beilner, U. Blomer, A. Schmiedl, I. Schmidt-Richter, M.L. Kruse, A. Haverich, U. Martin, No evidence of transdifferentiation of human endothelial progenitor cells into cardiomyocytes after coculture with neonatal rat cardiomyocytes. Circulation. (2006); 113, 1326-34.

I. Gruh, K. Schwanke, S. Wunderlich, U. Blomer, M. Scherr, A. Ganser, A. Haverich, U. Martin, Shuttle system allowing simplified cloning of expression cassettes into advanced generation lentiviral vectors, Biotechniques. 2005; 38, 530, 532, 534.

I. Gruh, U. Martin, Transdifferentiation of Stem Cells: A Critical View, in Engineering of Stem Cells, edited by U. Martin, Springer-Verlag GmbH, 2009.

A. Ruhparwar, F. Er, U. Martin, K. Radke, I. Gruh, M. Niehaus, M. Karck, A. Haverich, U.C. Hoppe, Enrichment of cardiac pacemaker-like cells: neuregulin-1 and cyclic AMP increase I(f)-current density and connexin 40 mRNA levels in fetal cardiomyocytes. Med Biol Eng Comput. 2007; Feb;45(2):221-7.

Unit 20 - W2 Bioartificial Lung

Zardo P, Zhang R, Wiegmann B, Haverich A, Fischer S: “Biological materials for diaphragmatic repair: initial experiences with the PeriGuard Repair PatchÒ” Thorac Cardiovasc Surg., 2011 Feb;59(1):40-4. Epub 2011 Jan 17.

Beutel G, Wiesner O, Eder M, Hafer C, Schneider AS, Kielstein JT, Kuehn C, Heim A, Ganzenmueller T, Kreipe H, Haverich A, Tecklenburg A, Ganser A, Welte T, Hoeper MM:  “Virus-associated hemophagocytic syndrome as a major contributor to death in patients with 2009 influenza A (H1N1) infection”, Critical Care, (2011), 15, R80.

Martens A, Gruh I, Dimitroulis D, Rojas SV, Schmidt-Richter I, Rathert C, Khaladj N, Gawohl A, Chikobava MG, Martin U, Haverich A, Kutschka I: “Rhesus monkey cardiosphere derived cells for myocardial restoration” (REBIRTH-acknowledgment), Cytotherapy, 2011, (Epub ahead)

Zweigerdt R, Olmer R, Singh H, Haverich A, Martin U: “Scalable expansion culture of human pluripotent stem cells in suspension culture” Nature Protocols, (2011), 6, 4, 1-12

Hess C, Herr C, Beiswenger C, Zakharkina T, Schmid RM, Bals R: “Myeloid RelA regulates pulmonary host defense networks” European Respiratory Journal, 2010, 35-2, 343-352

Hess C, Wiegmann B, Maurer A, Fischer P, Möller L, Martin U, Hilfiker A, Haverich A, Fischer S: „Surface endothelialization reduces thrombogenicity of poly 4-methyl-1-pentene (PMP gas exchange membranes“ Tissue Engineering Part A, 2010, 16-10, 3043-3053 (REBIRTH-acknowledgment)

Olsson KM, Simon A, Strueber M, Hadem J, Wiesner O, Gottlieb J, Fuehner T, Fischer S, Warnecke G, Kühn C, Haverich A, Welte T, Hoeper MM: „Extracorporeal Membrane Oxygenation in Nonintubated Patients as Bridge to Lung Transplantation“ Am.J.Transplant. (2010), 10, 9, 2173-2178

Fischer S, Hoeper M, Bein T, Simon A, Gottlieb J, Wisser W, Frey L, van Raemdonck D, Welte T, Haverich A, Strüber M. Interventional lung assist: a new concept of protective ventilation in bridge to lung transplantation. ASAIO J. 2008; Jan-Feb; 54:-3-10.

Meyer A, Strueber M, Fischer S. Advances in Extracorporeal Ventilation. Anesthesiology Clin 2008; Vol 26(2): 381–391

Fischer S, Bohn D, Rycus P, Pierre AF, dePerrot M, Waddell TK, Keshavjee S. Extracorporeal membrane oxygenation for primary graft dysfunction after lung transplantation: analysis of the Extracorporeal Life Support Organization (ELSO) registry. Journal of Heart and Lung Transplantation. 2007; May;26(5):472-7.

Fischer S, Hoeper M, Tomaszek S, Simon A, Gottlieb J, Welte T, Haverich A, Strueber M. Bridge to lung transplantation with the extracorporeal membrane ventilator Novalung in the veno-venous mode: the initial Hannover experience. ASAIO Journal. 2007; Mar-Apr;53(2):168-70

Fischer S, Simon AR, Welte T, Hoeper MM, Meyer A, Tessmann R, Gohrbandt B, Gottlieb J, Haverich A, Strueber M. Bridge to lung transplantation using the novel pumpless Interventional lung assist device NovaLung®. Journal of Thoracic and Cardiovascular Surgery 2006; 131(3):719-23.

Unit 21 - W2 Biomaterials

[1] M.C. Duran, S. Willenbrock, A. Barchanski, J.M. Muller, A. Maiolini, J.T. Soller, S. Barcikowski, I. Nolte, K. Feige, H. Murua Escobar, Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: efficiency and cytotoxicity, Journal of nanobiotechnology, 9 (2011) 47.

[2] J. Fitting, D. Killian, C. Junghanss, S. Willenbrock, H. Murua Escobar, S. Lange, I. Nolte, S. Barth, M.K. Tur, Generation of recombinant antibody fragments that target canine dendritic cells by phage display technology, Veterinary and comparative oncology, 9 (2011) 183-195.

[3] C.R. Henjes, S. Hungerblueher, I.B. Bojarski, I. Nolte, P. Wefstaedt, Assessment of left ventricular function by 64-detector-row computed tomography in healthy dogs: comparison with echocardiography, American Journal of Veterinary Research, in press (2011).

[4] C.R. Henjes, I. Nolte, P. Wefstaedt, Multidetector-row computed tomography of thoracic aortic anomalies in dogs and cats: patent ductus arteriosus and vascular rings., BMC Vet Res, 23 (2011) 57.

[5] A.A. Ismail, S. Wagner, H. Murua Escobar, S. Willenbrock, K.A. Sterenczak, M.T. Samy, A.M. Abd El-Aal, I. Nolte, P. Wefstaedt, Effects of High Mobility Group A Protein Application on Canine Adipose Derived Mesenchymal Stem Cell in vitro, Veterinary Medicine International, in press (2011).

[6] N. Reimann-Berg, H. Murua Escobar, Y. Kiefer, R. Mischke, S. Willenbrock, N. Eberle, I. Nolte, J. Bullerdiek, Cytogenetic analysis of CpG-oligonucleotide DSP30 plus Interleukin-2-Stimulated canine B-Cell lymphoma cells reveals the loss of one X Chromosome as the sole abnormality, Cytogenetic and genome research, 135 (2011) 79-82.

[7] S. Van der Heyden, S. Croubels, C. Gadeyne, R. Ducatelle, S. Daminet, H. Murua Escobar, K.A. Sterenczak, I. Polis, S. Schauvliege, M. Hesta, K. Chiers, Influencing Oral Prednisolone Bioavailability in Beagle Dogs by Modulation of P-glycoprotein expression, American Journal of Vetrinary Research, in press (2011).

[8] S. Willenbrock, O. Braun, J. Baumgart, S. Lange, C. Junghanss, A. Heisterkamp, I. Nolte, J. Bullerdiek, H. Murua Escobar, TNF-alpha stimulation triggers release of high mobility group box 1 (HMGB1) protein in mammary cells (MTH53A) of epithelial origin, Cytokine Ms., No CYTO-10270 (2011).

[9] K.A. Sterenczak, S. Kleinschmidt, P. Wefstaedt, N. Eberle, M. Hewicker-Trautwein, J. Bullerdiek, I. Nolte, H. Murua Escobar, Quantitative PCR and immunohistochemical analyses of HMGB1 and RAGE expression in canine disseminated histiocytic sarcoma (malignant histiocytosis), Anticancer research, 31 (2011) 1541-1548.

[10] K.A. Sterenczak, A.E. Joetzke, S. Willenbrock, N. Eberle, S. Lange, C. Junghanss, I. Nolte, J. Bullerdiek, D. Simon, H. Murua Escobar, High-mobility group B1 (HMGB1) and receptor for advanced glycation end-products (RAGE) expression in canine lymphoma, Anticancer research, 30 (2010) 5043-5048.

[11] A.E. Joetzke, K.A. Sterenczak, N. Eberle, S. Wagner, J.T. Soller, I. Nolte, J. Bullerdiek, H. Murua Escobar, D. Simon, Expression of the high mobility group A1 (HMGA1) and A2 (HMGA2) genes in canine lymphoma: analysis of 23 cases and comparison to control cases, Veterinary and comparative oncology, 8 (2010) 87-95.

[12] S. Petersen, J.T. Soller, S. Wagner, A. Richter, J. Bullerdiek, I. Nolte, S. Barcikowski, H. Murua Escobar, Co-transfection of plasmid DNA and laser-generated gold nanoparticles does not disturb the bioactivity of GFP-HMGB1 fusion protein, Journal of nanobiotechnology, 7 (2009) 6.

[13] A. Richter, G. Hauschild, H. Murua Escobar, I. Nolte, J. Bullerdiek, Application of high-mobility-group-A proteins increases the proliferative activity of chondrocytes in vitro, Tissue engineering. Part A, 15 (2009) 473-477.

[14] K.A. Sterenczak, S. Willenbrock, M. Barann, M. Klemke, J.T. Soller, N. Eberle, I. Nolte, J. Bullerdiek, H. Murua Escobar, Cloning, characterisation, and comparative quantitative expression analyses of receptor for advanced glycation end products (RAGE) transcript forms, Gene, 434 (2009) 35-42.

[15] D. Wolf, M. Lupke, P. Wefstaedt, K. Melichar, I. Nolte, H. Seifert, [Optimising MR image quality of the canine nose], Berliner und Munchener tierarztliche Wochenschrift, 122 (2009) 219-226.

[16] J.T. Soller, C. Beuing, H. Murua Escobar, S. Winkler, N. Reimann-Berg, N. Drieschner, G. Dolf, C. Schelling, I. Nolte, J. Bullerdiek, Chromosomal assignment of canine THADA gene to CFA 10q25, Molecular cytogenetics, 1 (2008) 11

[17] S. Altmann, S. Lange, J. Pommerencke, H. Murua Escobar, J. Bullerdiek, I. Nolte, M. Freund, C. Junghanss, High Mobility Group Box 1-Protein expression in canine haematopoietic cells and influence on canine peripheral blood mononuclear cell proliferative activity, Veterinary immunology and immunopathology, 126 (2008) 367-372.

[18] C. Beuing, J.T. Soller, M. Muth, S. Wagner, G. Dolf, C. Schelling, A. Richter, S. Willenbrock, N. Reimann-Berg, S. Winkler, I. Nolte, J. Bullerdiek, H. Murua Escobar, Genomic characterisation, chromosomal assignment and in vivo localisation of the canine high mobility group A1 (HMGA1) gene, BMC genetics, 9 (2008) 49.

[19] J. Jensen, D. Simon, H. Murua Escobar, J.T. Soller, J. Bullerdiek, P. Beelitz, K. Pfister, I. Nolte, Anaplasma phagocytophilum in dogs in Germany, Zoonoses and public health, 54 (2007) 94-101.

[20] B. Meyer, D. Krisponeit, C. Junghanss, H. Murua Escobar, J. Bullerdiek, Quantitative expression analysis in peripheral blood of patients with chronic myeloid leukaemia: correlation between HMGA2 expression and white blood cell count, Leukemia & lymphoma, 48 (2007) 2008-2013.

[21] J.T. Soller, H. Murua-Escobar, S. Willenbrock, M. Janssen, N. Eberle, J. Bullerdiek, I. Nolte, Comparison of the human and canine cytokines IL-1(alpha/beta) and TNF-alpha to orthologous other mammalians, The Journal of heredity, 98 (2007) 485-490.

Unit 22 - JRG Basic Mechanisms of Tissue Formation

K. Chinthalapudi, M.H. Taft, R. Martin, S.M. Heissler, M. Preller, F.K. Hartmann, H. Brandstaetter, J. Kendrick-Jones, G. Tsiavaliaris, H.O. Gutzeit, R. Fedorov, F. Buss, H.J. Knolker, L.M. Coluccio, D.J. Manstein, Mechanism and Specificity of Pentachloropseudilin-mediated Inhibition of Myosin Motor Activity, Journal of Biological Chemistry, 286 (2011) 29700-29708.

R.P. Diensthuber, M. Muller, S.M. Heissler, M.H. Taft, I. Chizhov, D.J. Manstein, Phalloidin perturbs the interaction of human non-muscle myosin isoforms 2A and 2C1 with F-actin, Febs Letters, 585 (2011) 767-771.

S.M. Heissler, D. Manstein, Functional characterization of the human myosin-7a, Cell Mol Life Sci, (2011).

S.M. Heissler, D.J. Manstein, Comparative Kinetic and Functional Characterization of the Motor Domains of Human Nonmuscle Myosin-2C Isoforms, Journal of Biological Chemistry, 286 (2011) 21191-21202.

M. Preller, S. Bauer, N. Adamek, S. Fujita-Becker, R. Fedorov, M.A. Geeves, D.J. Manstein, Structural Basis for the Allosteric Interference of Myosin Function by Reactive Thiol Region Mutations G680A and G680V, Journal of Biological Chemistry, 286 (2011) 35051-35060.

M. Preller, K. Chinthalapudi, R. Martin, H.J. Knolker, D.J. Manstein, Inhibition of Myosin ATPase Activity by Halogenated Pseudilins: A Structure-Activity Study, Journal of Medicinal Chemistry, 54 (2011) 3675-3685.

T.F. Reubold, S. Wohlgemuth, S. Eschenburg, Crystal Structure of Full-Length Apaf-1: How the Death Signal Is Relayed in the Mitochondrial Pathway of Apoptosis, Structure, 19 (2011) 1074-1083.

R. Forke, K.K. Gruner, K.E. Knott, S. Auschill, S. Agarwal, R. Martin, M. Bohl, S. Richter, G. Tsiavaliaris, R. Fedorov, D.J. Manstein, H.O. Gutzeit, H.J. Knolker, Total synthesis of biologically active alkaloids using transition metals, Pure and Applied Chemistry, 82 (2010) 1975-1991.

R. Fedorov, M. Bohl, G. Tsiavaliaris, F.K. Hartmann, M.H. Taft, P. Baruch, B. Brenner, R. Martin, H.J. Knolker, H.O. Gutzeit, D.J. Manstein, The mechanism of pentabromopseudilin inhibition of myosin motor activity, Nature structural & molecular biology, 16 (2009) 80-88.

T. Knoth, K. Warburg, C. Katzka, A. Rai, A. Wolf, A. Brockmeyer, P. Janning, T.F. Reubold, S. Eschenburg, D.J. Manstein, K. Hubel, M. Kaiser, H. Waldmann, The Ras pathway modulator melophlin A targets dynamins, Angewandte Chemie, 48 (2009) 7240-7245.

R. Martin, A. Jager, M. Bohl, S. Richter, R. Fedorov, D.J. Manstein, H.O. Gutzeit, H.J. Knolker, Total synthesis of pentabromo- and pentachloropseudilin, and synthetic analogues--allosteric inhibitors of myosin ATPase, Angewandte Chemie, 48 (2009) 8042-8046.

E. Stolboushkina, S. Nikonov, A. Nikulin, U. Blasi, D.J. Manstein, R. Fedorov, M. Garber, O. Nikonov, Crystal structure of the intact archaeal translation initiation factor 2 demonstrates very high conformational flexibility in the alpha- and beta-subunits. J Mol Biol. 2008; 382:680-91.

M.H. Taft, F.K. Hartmann, A. Rump, H. Keller, I. Chizhov, D.J. Manstein, G. Tsiavaliaris, Dictyostelium Myosin-5b is a conditional processive motor. J Biol Chem. 2008; 283:26902-10.

G. Tsiavaliaris, S. Fujita-Becker, U. Dürrwang, R.P. Diensthuber, M.A. Geeves, D.J. Manstein, Mechanism, Regulation, and Functional Properties of Dictyostelium Myosin-1B. J. Biol. Chem. 2008; 283:4520-27.

R. Fedorov, M. Böhl, G. Tsiavaliaris, F.K. Hartmann, M.H. Taft, P. Baruch, B. Brenner, R. Martin, H.J. Knölker, H.O. Gutzeit, D.J. Manstein, The mechanism of pentabromopseudilin inhibition of myosin motor activity. Nat. Struct. Mol. Biol. 2009; 16:80-88.

Unit 23 - JRG Polymer Design

Palecek, J., Zweigerdt, R., Olmer, R., Martin, U., Kirschning, A., Dräger, G. (2011) A practical synthesis of Rho-Kinase inhibitor Y-27632 and fluoro derivatives and their evaluation in human pluripotent stem cells. Organic and Biomolecular Chemistry; 9(15):5503-5510.

Ovsianikov, A.; Deiwick, A.; Van Vlierberghe, S.; Dubruel,P.; Möller, L.; Dräger, G.; Chichkov, B. (2011) Laser fabrication of 3D CAD Scaffolds from photosensitive gelatin for applications in tissue engineering. Biomacromolecules; 12(4):851-858.

Möller, L.; Krause, A., Dahlmann, J.; Gruh, I.; Kirschning, A.; Dräger, G. (2011) Preparation and evaluation of hydrogel-composites from methacrylated hyaluronic acid, alginate, and gelatin for tissue engineering. Int. J. Artif. Organs; 2011, 34(2):93-102.

Palecek, J., Dräger, G., Kirschning, A. (2011), A practical large-scale synthesis of cyclic RGD pentapeptides suitable for further functionalization through click' chemistry. Synthesis; (4):653-661.

Harmrolfs, K., Brünjes, M., Dräger, G., Floss, H.G., Sasse, F., Taft, F., Kirschning, A. (2010) Cyclization of synthetic seco-proansamitocins to ansamitocin macrolactams by actinosynnema pretiosum as biocatalyst. ChemBioChem; 11(18):2517-2520.

Su, Y., Kasper, C., Kirschning, A., Dräger, G., Berski, S. (2010) Synthesis of new polysialic acid derivatives. Macromolecular Bioscience; 10(9):1028-1033.

Haastert-Talini, K., Schaper-Rinkel, J., Schmitte, R., Bastian, R., Mühlenhoff, M., Schwarzer, D., Draeger, G., Su, Y., Scheper, T., Gerardy-Schahn, R, Grothe, C. (2010) In vivo evaluation of polysialic acid as part of tissue-engineered nerve transplants. Tissue Engineering - Part A; 16(10):3085-3098.

Olmer, R.; Haase, A.; Merkert, S.; Cui, W., Pale?ek, J.; Ran, C.; Kirschning, A.; Scheper, T.; Glage, S.; Miller, K.; Curnow, E. C.; Hayes, E. S.; Martin, U. (2010) Long term expansion of undifferentiated human iPS and ES cells in suspension culture using a defined medium. Stem Cell Research; 5(1):51-64.

Assmann, U., Szentivanyi, A., Stark, Y., Scheper, T., Berski, S., Dräger, G., Schuster, R.H.: Fiber scaffolds of polysialic acid via electrospinning for peripheral nerve regeneration (2010) Journal of Materials Science: Materials in Medicine 21: 2115-2124.

Berski, S.; van Bergeijk, J.; Schwarzer, D.; Stark, Y.; Kasper, C.; Scheper, T.; Grothe, C.; Gerardy-Schahn, R.; Kirschning, A.; Dräger, G.: Synthesis and Biological Evaluation of a Polysialic Acid-Based Hydrogel as Enzymatically Degradable Scaffold Material for Tissue Engineering (2008) Biomacromolecules 9: 2353-2359.

Hartlieb, S., Günzel, A., Gerardy-Schahn, R., Münster-Kühnel, A.K., Kirschning, A., Dräger, G.: Chemoenzymatic synthesis of CMP-N-acetyl-7-fluoro-7-deoxy-neuraminic acid (2008) Carbohydr. Res. 343: 2075-2082.

Haile, Y., Berski, S., Dräger, G., Nobre, A., Stummeyer, K., Gerardy-Schahn, R., Grothe, C.: The effect of modified polysialic acid based hydrogels on the adhesion and viability of primary neurons and glial cells (2008) Biomaterials 29: 1880-1891.

Dräger, G., Kiss, C., Kunz, U., Kirschning, A.: Enzyme-purification and catalytic transformations in a microstructured PASSflow reactor using a new tyrosine-based Ni-NTA linker system attached to a polyvinylpyrrolidinone-based matrix (2007) Org. Biomol. Chem. 5: 3657-3664.

Bruns, S., Stark, Y., Röker, S., Wieland, M., Dräger, G., Kirschning, A., Stahl, F., Kasper, C., Scheper, T.: Collagen biomaterial doped with colominic acid for cell culture applications with regard to peripheral nerve repair (2007) Journal of Biotechnology 131: 335-345.

Dräger, G., Jeske, F., Kunst, E., Lopez, E.G., Sanchez, H.V., Tsichritzis, F., Kirschning, A., Jakupovic, J.: Tonantzitlolone and other diterpenes from Stillingia sanguinolenta (2007) Eur. J. Org. Chem. 5020-5026.

Haile, Y., Haastert, K., Cesnulecicius, K., Stummeyer, K., Timmer, M., Berski, S., Dräger, G., Gerardy-Schahn, R. and Grothe, C.: Culturing of glial and neuronal cells on polysialic acid (2007) Biomaterials 28: 1163-1173.

Dräger, G.; Dumitriu, S.; Krause, A.; Möller, L. (2010) Carbohydrates. In: Handbook of Biodegradable Polymers, Schröter, M.; Lendlein, A. (Eds.), Wiley-VCH, Weinheim, pp 155-193.

Unit 24 - W3 Biomedical Process Technology

Stoll C, Holovati JL, Acker JP, Wolkers WF. Liposomes composed of unsaturated lipids for membrane modification of human erythrocytes. Molecular Membrane Biology. 2011; doi:10.3109/09687688.2011.622304

Stadnick H, Stoll C, Wolkers WF,  Acker JP, Holovati JL. The effect of liposome treatment on the quality of hypothermically stored red blood cells. Biopreservation and Biobanking. 2011; in press

Diekmann U, Spindler R, Wolkers WF, Glasmacher B, Müller T. Cryopreservation and quality control of mouse embryonic feeder cells. Cryobiology 2011; 63, 104-110.

Röpcke T, Oldenhof H, Leiding C, Sieme H, Bollwein H, Wolkers WF. Liposomes for cryopreservation of bull sperm. Theriogenology. 2011; 76, 1465–1472.

Akhoondi M, Oldenhof H, Stoll C, Sieme H, Wolkers WF. Membrane hydraulic permeability changes during cooling of mammalian cells. Biochimica et Biophysica Acta. 2011; 1808, 642–648.

Stoll C, Wolkers WF. Membrane stability during biopreservation of blood cells. Transfusion medicine and hemotherapy. 2011; 38, 89–97.

Stoll C, Stadnick H, Kollas O, Holovati JL, Glasmacher B, Acker JP, Wolkers WF. Liposomes alter thermal phase behavior of red blood cell membranes. Biochimica et Biophysica Acta. 2011; 1808, 474-481.

Oldenhof H, Blässe AK, Wolkers WF, Bollwein H, Sieme H. Osmotic properties of subpopulations of stallion sperm. Theriogenology 2011; 76, 386-391.

Spindler R, Wolkers WF, Glasmacher B. Dimethylsulfoxide and ethylene glycol promote membrane dehydration during cryopreservation. CryoLetters. 2011; 32, 148-157.

Venkatasubramanian R, Wolkers WF, Shenoi M, Barocas V, Lafontaine D, Soule C, Iaizzo P, Bischof JC. Freeze–Thaw Induced Biomechanical changes in arteries: Role of collagen matrix and smooth muscle cells. Annals of Biomechanical Engineering. 2010; 38, 694-706.

Hagiwara M, Choi J, Devireddy RV, Roberts KP, Wolkers WF, Makhlouf A, Bischof JC. Cellular biophysics during freezing of rat and mouse sperm predicts post-thaw motility. Biology of Reproduction. 2009; 81, 700–706.

Shah N, Wolkers WF, Morrissey M, Sun WQ, Bischof JC. Fourier transform infrared spectroscopy investigation of native tissue matrix modifications using a gamma irradiation process. Tissue Engineering Part C. 2009; 15, 33-40.

Balasubramanian SK, Wolkers, WF, Bischof JC. Membrane hydration correlates to cellular biophysics during freezing in mammalian cells. Biochimica et Biophysica Acta. 2009; 1788, 945-953.

Wolkers WF, Balasubramanian SK, Ongstad EL, Zec H, Bischof JC. Effects of freezing on membranes and proteins in LNCaP prostate tumor cells. Biochimica et Biophysica Acta. 2007; 1768, 728-736.

Unit 25 - JRG Biothermodynamics

Bernemann I, Mueller T, Blasczyk R, Glasmacher B, Hofmann N: Colonization of collagen scaffolds by adipocytes derived from mesenchymal stem cells of the common marmoset monkey. Biochem Biophys Res Commun.: 411(2): 317-22, 2011.

Hofmann N, Bernemann I, Pogozhykh D, Glasmacher B: Development of systematic parameter optimization for cryopreservation regimens for cellular suspensions. Problems of Cryobiology, Vol 21, No 2, 2011 (ISSN: 0233-7673), in press.

Freimark D, Sehl C, Weber C, Hudel K, Czermak P, Hofmann N, Spindler R, Glasmacher B: Systematic parameter optimization of a Me2SO- and serum-free cryopreservation protocol for human mesenchymal stem cells, Cryobiology: 63 (2), 67-75, 2011.

Diekmann U, Spindler R, Wolkers W F, Glasmacher B, Müller T: Cryopreservation and quality control of mouse embryonic feeder cells, Cryobiology: 63 (2), 104-110, 2011.

Wiedemann A, Schwarmborn J, Honndorf S, Bernemann I, Figureido C, Schwarmbach A, Blasczyk R, Müller T: Induced pluripotent stem cells of the common marmoset monkey display high cellular plasticity. Regenerative Medicine 6 (2), 244, 2011.

Pogozhykh D, Hofmann N, Müller T, Glasmacher B: Development of optimal cryopreservation protocol for suspensions of MSCs with application of systematic parameter optimization. Regenerative Medicine 6 (2), 229, 2011.

Bernemann I, Deiwick A, Hofmann N, Grüne M, Koch L, Glasmacher B: Cell seeding of collagen scaffolds via cell printing. Regenerative Medicine 6 (2), 43, 2011.

Bernemann I, Deiwick A, Marx C, Mueller T, Glasmacher B, Hofmann N: Adipogenic differentiation potential of MSCs of the common marmoset monkeys in collagen scaffolds. Regenerative Medicine 6 (2),189, 2011.

Bernemann I, Spindler R, Manuchehrabadi N, Choi J, Wolkers W, Bischof J, Glasmacher B: Visualization of DMSO diffusion in engineering constructs for long-term storage. Regenerative Medicine 6 (2), 343, 2011.

Wiedemann A, Schwarmborn J, Bernemann I, Figureido C, Honndorf S, Schwarmbach A, Blasczyk R, Müller T: Induced pluripotent stem cells of the common marmoset monkey. Transfu Med Hemother 38 (1),19-20, 2011.

Bernemann I, Spindler R, Manuchehrabadi N, Choi J H, Wolkers W, Bischof J, Glasmache B: Long-term storage of engineered tissues: Visualization of CPA diffusion in collagen scaffolds. Histol and Histopathol 26 (1), 303, 2011.

Bernemann I, Deiwick A, Hofmann N, Grüne M, Koch L, Chichkov B, Glasmacher B: Cell seeding of collagen scaffolds via LIFT. TERMIS EU-2010, Abstract, ISBN: 978-0-95644492-0-7, 2010.

Bernemann I, Deiwick A, Marx C, Müller T, Glasmacher B, Hofmann N: Adipogenic differentiation potential of mesenchymal stem cells of the common marmoset monkey in collagen scaffolds. TERMIS EU-2010, ISBN 978-0-9564492-0-7, 2010. (‘REBIRTH-acknowledgement’)

http://lib.bioinfo.pl/pmid:20673023Gruene M, Deiwick A, Koch L, Schlie S, Unger C, Hofmann N, Bernemann I, Glasmacher B, Chichkov B: Laser printing of stem cells for biofabrication of scaffold-free autologous grafts. Tissue Engineering Part C 17(1):79-87, 2010.

Bernemann I, Manuchehrabadi N, Spindler R, Choi J, Wolkers W, Bischof J, Glasmacher B: Diffusion of dimethyl sulfoxide in tissue-engineered collagen scaffolds visualized by computer tomography. Cryoletters 31(6), 493-503, 2010.http://www.ifv.uni-hannover.de/picture/upload/file/DMSO%20paper/CryoLetters31%206%20pp493-503.pdf

Sehl C, Freimark D, Weber C, Hudel K, Hofmann N, Glasmacher B, Czermak P: Entwicklung eines GMP-gerechten Verfahrens zur Kryokonservierung von Zellen und zellbasierten Implantaten für die regenerative Medizin. DKV-Jahresbericht 37, Band 1, ISBN 978-3-932715-46-4, 2010

Sun H, Hofmann N, Glasmacher B: Application of compatible solute within cryopreservation of human endothelial cells. CryoLetters 31(2), 2010

Bernemann I, Spindler R, Manuchehrabadi N, Choi J, Wolkers W, Bischof J, Glasmacher B: Dimethyl sulfoxide diffusion in tissue-engineered collagen scaffolds visualized by computer tomography. CryoLetters 31(2), 2010.

Wolkers W F, Diekmann U, Müller T, Spindler R, Glasmacher B: Cryomicroscopy and FTIR studies on gamma treated mouse embryonic fibroblast feeder cells during freezing, CryoLetters 31(2), 2010

Spindler R, Glasmacher B: Cryopreservation of human pulmonary endothelial cells: Determination of osmotic parameters, CryoLetters 31(2), 2010

Bernemann I, Manuchehrabadi N, Spindler R, Choi J, Wolkers W, Bischof J, Glasmacher B: Diffusion of dimethyl sulfoxide in tissue-engineered collagen scaffolds visualized by computer tomography. CryoLetters 2010;31(6):493-503. (submitted, 04/2010).

Spindler R, Glasmacher B: Kryomikroskopie: Eine Methode zur Visualisierung von Gefrierprozessen, KI Kälte-Luft-Klimatechnik 4: 28-31, 2009.

Spindler R, Wolkers W F, Glasmacher B: Effect of Me2SO on membrane phase behavior and protein denaturation of human pulmonary endothelial cells studied by in situ FTIR spectroscopy, ASME, Journal of Biomechanical Engineering 131(7):074517-19, 2009.

Bernemann I, Sun H, Hofmann N, Glasmacher B. Increase of cell survival of native tissues and tissue engineered products by optimisation of the cryopreservation process. CryoLetters 30(1): 82, 2009.

Sun H, Hofmann N, Glasmacher B: Proline improves the cryopreservation of human endothe-lial cells for TE . Int. J. Artif.Organs: 32(7) 412, 2009,

Bernemann I, Deiwick A, Hofmann N, Grüne M, Koch L, Chichkov B, Glasmacher B: Laser-induced cell seeding of collagen scaffolds. Int. J. Artif.Organs 32(7): 416, 2009.

Hofmann N, Stoll C, Bernemann I, Glasmacher B.: Improvement of human pulmonary endothelial cells (HPMEC). CryoLetters 30(2): 156, 2009.

Sun H, Hofmann N, Glasmacher B: Biomolecules for improvement of cryopreservation of human endothelial cells. Cryobiology 59(3): 397, 2009.

Evertz F, Blume H, Glasmacher B: µ-Cryopreservation. Int. J. Artif.Organs 32(7): 412, 2009

Bernemann I, Hofmann N, Szentivanyi A, Kuberka M, Glasmacher B: Optimierung von Kryokonservierungsprotokollen: Systematische Parameteranalyse. KI Kälte-Luft-Klimatechnik KI-01-02/2008: 24-27, 2008.

Bernemann I, Hofmann N, Glasmacher B. Optimisation of thawing parameters to improve cryopreservation of cells and tissue engineered products. Int. J. Artif. Organs 31(7): 637, 2008.

Bernemann I, Hofmann N, Spindler R, Szentivanyi A, Glasmacher B. Strategy to improve cryopreservation protocols. CryoLetters 29(1) Abstracts, 73-88, 2008.

Bernemann I, Hofmann N, Glasmacher B. Optimisation of the thawing process improves the cryopreservation of cell suspension and tissue engineered products. Cryobiology 57, 337, 2008.

Evertz F, Szentivanyi A, Bernemann I, Hofmann N, Glasmacher B. Development of a new controlled rate freezing device for systematic parameter optimization in cryopreservation. Cryobiology 57(3): 337, 2008.

Hofmann N, Sun H, Bernemann I, Glasmacher B. Influence of Temperature Profile and CPA Distribution on Cryopreservation of Native Tissue. Cryobiology 57, 330, 2008.

Hofmann N, Bernemann I, Glasmacher B. Cryopreservation of tissue and tissue engineered products. Int. J. Artif. Organs. 31(7): 583, 2008.

Spindler R, Hofmann N, Glasmacher B: Controlled ice nucleation during cryopreservation, Cryobiology 57(3): 337, 2008.

Stoll C, Bernemann I, Hofmann N, Glasmacher B. Systematic Optimisation of Parameters for Cryopreservation of Human Pulmonal Endothelial Cells (HPMEC): Difference in Cell Survival Rates Analyzed by Recultivation vs. Trypanblue Staining. Cryobiology 57: 337-338, 2008.

Bernemann I, Hofmann N, Szentivanyi A, Glasmacher B. A systematic parametric analysis to improve cryopreservation of cells in suspension. Int. J. Artif.Organs. 30(8): 712, 2007.

Bernemann I, Hofmann N, Szentivanyi A, Glasmacher B. Strategy to improve cryopreservation protocols: A systematic parametric analysis for suspended cells. Cryobiology 55, (3): 338, 2007.

Hofmann N, Bernemann I, Glasmacher B. Kryokonservierung von Gewebe. DKV-Jahresbericht 34, Band 1-I3, ISBN-3-932715-41-1, 2007.

Unit 26 - RG Biofluid Mechanics

Bernemann I, Manuchehrabadi N, Spindler R, Choi J,  Wolkers W, Bischof J,  Glasmacher B: Diffusion of dimethyl sulfoxide in tissue-engineered collagen scaffolds visualized by computer tomography. CryoLetters 2010; 31 (6):493-503 

Chakradeo T; Szentivanyi A; Glasmacher B: Growth Factor Release from Electrospun Polymer Fibres for Tissue Engineering. International Journal of Artificial Organs 2009, 32 (7), 459.

Gruene M, Deiwick A, Koch L, Schlie S, Unger C, Hofmann N, Bernemann I, Glasmacher B,  Chichkov B: Laser printing of stem cells for biofabrication of scaffold-free autologous grafts. Tissue Engineering Part C Methods, 2011; 17(1): 79-87 

H. Zernetsch, T. Chakradeo, A. Szentivanyi, B. Glasmacher, H. Menzel, P. Behrens: Träger, Tracer und Depots: Submikron- und Nanotechnologie in der Biomedizin. Tagungsband „Kunststoffe in der Medizintechnik“, ISBN 978-3-18-234309-7, VDI Verlag GmbH, Düsseldorf, 2010

Krings M., Kanellopoulou D., Koutsoukos P. D., Mavrilas D., Glasmacher B.: Development of a new combined test set-up for accelerated dynamic pH controlled in vitro calcification of  porcine heart valves. Int J Artif Organs 2009;39:794-801

Szentivanyi A, Chakradeo T, Zernetsch H, Glasmacher B: Electrospun cellular microenvironments: Understanding controlled release and scaffold structure. Adv. Drug Deliv.Rev. (2010) doi10.1016/ j.addr.2010.12.002 

Szentivanyi A, Zernetsch H, Menzel H, Glasmacher B: A review of developments in electrospinning technology: New Opportunities fort he design of artificial tissue structures.  Int. J Artif Organs 2011 (accepted for publication Jan 2011)

Wadouh T, Mavrilas D, Krings M, Bach F-W, Glasmacher B: Explantatanalysen klinischer Herzklappenbioprothesen. BIOmaterialien 9 (3), 2007, p. 230 

Unit 27 - W2 Biophotonics (see AG 5)

Unit 28 - W3 Nanoengineering

The REBIRTH research groups No. 29, 30, and 31 belong to this full professorship nanoengineering. See relevant publications also there.

R. Narayan, A. Doraiswamy, D. Chrisey, B. Chichkov, Materials Today, Medical Prototyping using two-photon polymerization, v. 13 (2010), 42-48.

K. Obata, J. Koch, U. Hinze, B. Chichkov, Multi-focus two-photon polymerization technique based on individually controlled phase modulation, Opt. Express v. 18 (2010), 17193-17200.

M. Farsari, B.N. Chichkov, Two-photon polymerization, Nat. Photonics, 3(2009), 450-452

Hartmann N, Franzka S, Koch J, Ostendorf A, Chichkov BN, Subwavelength patterning of alkylsiloxane monolayers via nonlinear processing with single femtosecond laser pulses, Appl. Phys. Lett. 2008; 92, 223111.

Kuznetsov AI, Koch J, Chichkov BN, Nanostructuring of thin gold films by femtosecond lasers, Appl. Phys. A. 2009; 94, 221.

Heinroth F, Bremer I, Muenzer S, Behrens P, Reinhardt C, Passinger S, Ohrt C, Chichkov BN, Microstructured templates produced using femtosecond laser pulses for the deposition of mesoporous silicas, Microporous and Mesoporous Materials. 2009; 119, 104.

Gittard SD, Ovsianikov A, Monteiro-Riviere NA, Lusk J, Morel P, Minghetti P, Lenardi C, Chichkov BN, Narayan RJ, Fabrication of polymer microneedles using a two-photon polymerization and micromolding process, J. Diabetes. Sci. Technol. 2009; 3, 305.

Dinca V, Kasotakis E, Catherine J, Mourka A, Ranella A, Ovsianikov A, Chichkov BN, Farsari M, Mitraki A, Fotakis C. Directed Three-Dimensional, Patterning of Self-Assembled Peptide Fibrils. Nano Lett. 2008; 8, 538-43.

Ovsianikov A, Schlie S, Ngezahayo A, Haverich A, Chichkov BN. Two-photon polymerization technique for microfabrication of CAD designed three-dimensional scafolds from commercially available photosensitive materials. J Tissue Eng Regen Med. 2007; 1, 443-9.

Barcikowski S, Hahn A, Kabashin AV, Chichkov BN. Properties of nanoparticles generated during femtosecond laser machining in air and water. Appl. Phys. 2007; A 87, 47-55.

Ovsianikov A, Ostendorf A, Chichkov BN. Three-dimensional photofabrication with femtosecond lasers for applications in photonics and biomedicine., Applied Surface Science. 2007; 253, 6599-602.

Schlie S, Ngezahayo A, Ovsianikov A, Fabian T, Kolb H-A, Haferkamp H, Chichkov BN. Three-dimensional cell growth on structures fabricated from ORMOCER by two-photon polymerization technique. J Biomater Appl. 2007; 22, 275-87.

Unit 29 - RG Biological Laser Printing

M Gruene, M Pflaum, C Hess, S Diamantouros, S Schlie, A Deiwick, L Koch, M Wilhelmi, S Jockenhoevel, A Haverich, B Chichkov, Laser Printing of Three-Dimensional Multicellular Arrays for Studies of Cell–Cell and Cell–Environment Interactions, Tissue Engineering Part C Methods, 17(10):973-82 (2011)

Gaebel R, Ma N, Liu J, Guan J, Koch L, Klopsch C, Gruene M, Toelk A, Wang W, Mark P, Wang F, Chichkov B, Li W, Steinhoff G, Patterning human stem cells and endothelial cells with laser printing for cardiac regeneration, Biomaterials 32(35):9218-30 (2011)

M Gruene, A Deiwick, L Koch, S Schlie, C Unger, N Hofmann, I Bernemann, B Glasmacher, B Chichkov, Laser printing of stem cells for biofabrication of scaffold-free autologous grafts, Tissue Engineering Part C Methods, 17(1): 79-87 (2011)

M Gruene, M Pflaum, A Deiwick, L Koch, S Schlie, C Unger, M Wilhelmi, A Haverich and B N Chichkov, Adipogenic differentiation of laser-printed 3D tissue grafts consisting of human adipose-derived stem cells, Biofabrication, 3: 015005 (2011) Featured Article 

M Gruene, C Unger, L Koch, A Deiwick, B Chichkov, Dispensing pico to nanolitre of a natural hydrogel by laser-assisted bioprinting, Biomedical Engineering Online, 2011, 10:19 (2011), Highly Accessed Article

L Koch, S Kuhn, H Sorg, M Gruene, S Schlie, R Gaebel, B Polchow, K Reimers, S Stoelting, N Ma, PM Vogt, G Steinhoff, B Chichkov, Laser Printing of Skin Cells and Human Stem Cells, Tissue Engineering Part C Methods, 16(5):847-54 (2010)

A Ovsianikov, M Gruene, M Pflaum, L Koch, F Maiorana, M Wilhelmi, A Haverich, B Chichkov, Laser printing of cells into 3D scaffolds, Biofabrication 2 014104 (2010)

C Unger, M Gruene, L Koch, J Koch, B Chichkov, Time-resolved imaging of hydrogel printing via laser-induced forward transfer, Appl Phys A, DOI: 10.1007/s00339-010-6030-4 (2010)

 C Unger, M Gruene, L Koch, J Koch, B Chichkov, Time-resolved imaging of hydrogel transport by laser-induced forward transfer (LIFT), Proceedings of LPM2010 (2010)

Unit 30 - JRG Nanoparticles

A. Hahn, J. Fuhlrott, A. Loos, S. Barcikowski: Cytotoxicity and ion release of alloy nanoparticles. Submitted to J. Nanopart. Res., 2011.

A. Barchanski, N. Hashimoto, S. Petersen, C. L. Sajti, S. Barcikowski: Impact of strand length and binding orientation on the oligonucleotide attachment to the surface of laser-generated gold nanoparticles. Submitted to Bioconjugate Chemistry, 2011.

M. C. Duran, S. Willenbrock, A. Barchanski, J.-M. V. Müller, A. Maiolini, J. T. Soller, S. Barcikowski, I. Nolte, K. Feige, H. M. Escobar: Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: efficiency and cytotoxicity. J. Nanobiotech., 2011, 9:47, DOI:10.1186/1477-3155-9-47.

A. Barchanski, C. L. Sajti, C. Sehring, S. Petersen, S. Barcikowski: Design of bi-functional bioconjugated gold nanoparticles by pulsed laser ablation with minimized degradation. JLMN, 2011, 6(2), 124-130, DOI: 10.2961/jlmn.2011.02.0006.

A. Hahn, G. Brandes, P. Wagener, S. Barcikowski: Metal ion release kinetics from nanoparticle silicone composites. J. Control. Release, 2011, 154(2), 164-170, DOI:10.1016/j.jconrel.2011.05.023.

A. Barchanski, U. Taylor, S. Klein, S. Petersen, D. Rath, S. Barcikowski: Golden perspective: Application of Laser-Generated Gold Nanoparticle Conjugates in Reproductive Biology. Reprod. Domest. Anim., 2011, 46(s3), 42-52, DOI: 10.1111/j.1439-0531.2011.01844.x.

S. Petersen, A. Barchanski, U. Taylor, S. Klein, D. Rath, and S. Barcikowski: Penetratin-Conjugated Gold Nanoparticles ? Design of Cell-Penetrating Nanomarkers by Femtosecond Laser Ablation. J. Phys. Chem. C, 2011, 115(12), 5152-5159, DOI:10.1021/jp1093614.

P. Wagener, G. Brandes, A. Schwenke, S. Barcikowski: Impact of in situ polymer coating on particle dispersion into solid laser-generated nanocomposites. Phys. Chem. Chem. Phys., 2011, 13(11), 5120-5126, DOI: 10.1039/c0cp02262d.

C. L. Sajti, A. Barchanski, P. Wagener, S. Klein, S. Barcikowski: Delay Time and Concentration Effects During Bioconjugation of Nanosecond Laser-Generated Nanoparticles in Liquid Flow. J. Phys. Chem. C, 2011, 115(12), 5094-5101, DOI:10.1021/jp1093405. 

J. Jakobi, A. Menendez-Manjon, P. Wagener, S. Barcikowski: Stoichiometry of alloy nanoparticles from laser ablation of PtIr in acetone and their electrophoretic deposition on PtIr. Nanotechnology, 2011, 22(14), 145601, DOI:10.1088/0957-4484/22/14/145601.

Menendez-Manjon, P. Wagener, S. Barcikowski: Transfer-Matrix Method for Efficient Ablation by Pulsed Laser Ablation and Nanoparticle Generation in Liquids. J. Phys. Chem. C, 2011, 115(12), 5108-5114, DOI:10.1021/jp109370q.

A. Hahn, S. Günther, P. Wagener, S. Barcikowski: Electrochemistry-controlled metal ion release from silicone elastomer nanocomposites through combination of different metal nanoparticles. J. Mater. Chem., 2011, 21(28), 10287-10289, DOI:10.1039/C0JM04480F.

C. L. Sajti, S. Petersen, A. Menéndez-Manjón, S. Barcikowski: In-Situ Bioconjugation in Stationary Media and in Liquid Flow by Femtosecond Laser Ablation. Appl. Phys. A-Mater., 2011, 101:259­-264, DOI:10.1007/s00339-010-5813-y.

S. Barcikowski, A. Hahn, M. Guggenheim, K. Reimers, A. Ostendorf: Biocompatibility of nanoactuators: stem cell growth on laser-generated nickel-titanium shape memory alloy nanoparticles. J. Nanopart. Res., 2010, 12:1733-1742, DOI:10.1007/s11051-009-9834-4.

A. Hahn, T. Stöver, G. Paasche, M. Löbler, K. Sternberg, H. Rohm, S. Barcikowski: Therapeutic Window for Bioactive Nanocomposites Fabricated by Laser Ablation in Polymer-Doped Organic Liquids. Adv. Eng. Mater., 2010, 12:B156-B162, DOI:10.1002/adem.200980071.

A. Menendez-Manjon, S. Barcikowski, G. A. Shafeev, V. I. Mazhukin, B. N. Chichkov: Influence of beam intensity profile on the aerodynamic particle size distributions generated by femtosecond laser ablation. Laser and Particle Beams, 2010, 28:45-52, DOI:10.1017/S0263034609990553.

J. Walter, S. Petersen, U. Taylor, D. Rath, S. Barcikowski: Laser Ablation-Based One-Step Generation and Bio-Functionalization of Gold Nanoparticles Conjugated with Aptamers, J. Nanobiotech, 2010, 8:21, DOI:10.1186/1477-3155-8-21.

A. Menendez-Manjon, B. N. Chichkov, S. Barcikowski: Influence of Water Temperature on the Hydrodynamic Diameter of Gold Nanoparticles from Laser Ablation. J. Phys. Chem. C, 2010, 114:2999-2504, DOI:10.1021/jp909897v.

J. Jakobi, S. Petersen, A. Menendez-Manjon, P. Wagener, S. Barcikowski: Magnetic Alloy Nanoparticles from Laser Ablation in Cyclopentanone and Their Embedding into a Photoresist. Langmuir, 2010, 26:6892-6897, DOI:10.1021/la101014g.

A. Menendez-Manjon, S. Barcikowski: Hydrodynamic size distribution of gold nanoparticles controlled by repetition rate during pulsed laser ablation in water. Appl. Surf. Sci., 2010, 257:4285-4290, DOI:10.1016/j.apsusc.2010.12.037.

U. Taylor, S. Klein, S. Petersen, S. Barcikowski, D. Rath: Non-endosomal uptake of cellular uptake of ligand-free, positively charged gold nanoparticles, Cytometry, 2011, 77(5), 439-446, DOI:10.1002/cyto.a.20846.

S. Klein, S. Petersen, U. Taylor, D. Rath, S. Barcikowski: Quantitative visualisation of colloidal and intracellular gold nanoparticles by confocal microscopy, J. Biomed. Opt., 2010, 15(3):036015, DOI:10.1117/1.3461170.

S. Barcikowski, J. Walter, A. Hahn, J. Koch, H. Haloui, T. Herrmann, A. Gatti: Picosecond and femtosecond laser machining may cause health risks related to nanoparticle emission, JLMN, 2009, 4(3), 159-164, DOI:10.2961/jlmn.2009.03.0003.

S. Barcikowski, F. Devesa, K. Moldenhauer: Impact and strucutre of literature on nanoparticle generation by laser ablation in liquids, J. of Nanopart. Res., 2009, 11(8), 1883-1893, DOI:10.1007/s11051-009-9765-0.

N. Bärsch, J. Jakobi, S. Weiler, S. Barcikowski: Pure colloidal metal and ceramic nanoparticles from high-power picosecond laser ablation in water and acetone, Nanotechnology, 2009, 20:445603, DOI:10.1088/0957-4484/20/44/445603.

A. Menéndez-Manjón, J. Jakobi, K. Schwabe, J. K. Krauss, S. Barcikowski: Mobility of nanoparticles generated by femtosecond laser ablation in liquids and its application to surface patterning, JLMN, 2009, 4(2), 95-99, DOI:10.2961/jlmn.2009.02.0004.

N. Bärsch, A. Gatti, R. Sattari, S. Barcikowski: Improving Laser Ablation of Zirconia by Liquid Films: Multiple Influences of Liquids on Surface Machining and Nanoparticle Generation, JLMN, 2009, 4(1), 66-70, DOI:10.2961/jlmn.2009.01.0013.

S. Petersen, S. Barcikowski. In-situ Bioconjugation - Single Step Approach to Tailored Nanoparticle-Bioconjugates by Ultrashort Pulsed Laser Ablation. Adv. Funct. Mater, 2009, 19, 1167-1172, DOI:10.1002/adfm.200801526.

A. Hahn, S. Barcikowski. Production of Bioactive Nanomaterial using Laser Generated Nanoparticles. JLMN, 2009, 4(1), 51-4, DOI:10.2961/jlmn.2009.01.0010.

S. Petersen, J. Jakobi, A. Hörtinger, S. Barcikowski: In-Situ Conjugation - Tailored Nanoparticle-Conjugates by Laser Ablation in Liquids, JLMN, 2009, 4, 71-74, DOI:10.2961/jlmn.2009.01.0014.

S. Petersen, J. Jakobi, S. Barcikowski: In-situ bioconjugation of nanoparticles – Novel laser based approach to pure nanoparticle-conjugates. Appl. Surf. Sci., 2009, 255, 5435-5438, DOI:10.1016/j.apsusc.2008.08.064.

S. Petersen, S. Barcikowski: Conjugation Efficiency of the Laser-based Bioconjugation of Gold Nanoparticles with Nucleic Acids, J. Phys. Chem. C, 2009, 113, 19830-19835, DOI:10.1021/jp905962f.

S. Petersen, J. T. Soller, S. Wagener, A. Richter, J. Bullerdiek, I. Nolte, S. Barcikowski, H. M. Escobar: Co-transfection of Plasmid DNA and Laser-generated Gold Nanoparticles Does Not Disturb the Bioactivity of GFP-HMGB I Fusion Protein, J. Nanobiotech., 2009, 7(6), DOI:10.1186/1477-3155-7-6.

A. Hahn, S. Barcikowski, B. N. Chichkov. Influences on nanoparticle production during pulsed laser ablation. JLMN, 2008, 3(2), 73-77, DOI:10.2961/jlmn.2008.02.0003.

N. Bärsch, S. Barcikowski, K. Baier: Ultrafast-laser-processed zirconia and its adhesion to dental cement, JLMN, 2008, 3(2), 78-83, DOI:10.2961/jlmn.2008.02.0004.

R. Sattari, C. Dieling, S. Barcikowski, B. N. Chichkov: Laser-based fragmentation of microparticles for nanoparticle generation, JLMN, 2008, 3(2), 100-105, DOI:10.2961/jlmn.2008.02.0008.

S. Barcikowski, A. Hahn, A. V. Kabashin, B. N. Chichkov. Properties of nanoparticles generated during femtosecond laser machining in air and water. Appl. Phys. A, 2007, 87, 47–55, DOI:10.1007/s00339-006-3852-1.

S. Barcikowski, A. Menéndez-Manjón, B. N. Chichkov, M. Brikas, G. Raciûkaitis. Generation of nanoparticle colloids by picosecond and femtosecond laser ablation in liquid flow. Appl. Phys. Lett, 2007, 91:083113, DOI:10.1063/1.2773937.

A. Hahn, S. Barcikowski, A. Ostendorf: The nanoparticle risk during pulsed laser ablation, The Laser User, 2007, 47, 32-33.

S. Barcikowski, A. Hahn, B. N. Chichkov. Nanoparticles as Potential Risk during Femtosecond Laser Ablation, J. Laser Appl., 2007, 19(2), 65-73, DOI:10.2351/1.2402526.

L. L. Sartinska, S. Barcikowski, N. Wardenga, B. M. Rud, I. I. Timofeeva: Laser induced modification of surface structures, Appl. Surf. Sci., 2007, 253(9), 4295-4299, DOI:10.1016/j.apsusc.2006.09.032.

Unit 31 - RG Nanosurfaces

A. Ovsianikov, M. Malinauskas, S. Schlie, B. Chichkov, S. Gittard, R. Narayan, M. Löbler, K. Sternberg, K. Schmitz, A, Haverich, Three-dimensional laser micro- and nanostructuring of acrylated poly(ethylene glycol) materials and evolution of their cytoxicity for tissue engineering applications, Acta Biomaterialia, v. 7, (2011), 967-974.

A. Ovsianikov, A. Deiwick, S. Vlierberghe, M. Pflaum, M. Wilhelmi, P. Dubruel, B. Chichkov, Laser fabrication of 3D gelatin scaffolds for the generation of bioartificail tissues, Materials v. 4 (2011), 288-299.

A. Ovsianikov, A. Deiwick, S. Vlierberghe,  P. Dubruel, L. Möller, G. Dräger,  B. Chichkov, Laser fabrication of three-dimensional CAD  scaffolds from photosensitive gelatin for applications in tissue engineering, Biomacromolecules v. 12 (2011), 851-858.

A. Koroleva, S. Schlie, E. Fadeeva, S. Gittard, P. Miller, A. Ovsianikov, J. Koch, R. Narayan, B. Chichkov, Microreplication of laser-fabricated surface and three-dimensional structures, J. Opt. v. 12, (2010), 124009

Ovsianikov A, Shizhou X, Farsari M, Vamvakaki M, Fotakis C, Chichkov BN. Shrinkage of microstructures produced by two-photon polymerization of Zr-based hybrid photosensitive materials, Optics Express, 17, 4, 2143-2148; 2009.

Claeyssens F, Hasan EA, Gaidukeviciute A, Achilleos DS, Ranella A, Reinhardt C, Ovsianikov A, Shizhou X, Fotakis C, Vamvakaki M, Chichkov BN, Farsari M. Three-Dimensional Biodegradable Structures Fabricated by Two-Photon Polymerization, Langmuir, 25, 5, 3219-3223; 2009.

Ovsianikov A, Viertl J, Oubaha M, MacCraith B, Sakellari I, Giakoumaki A, Gray D, Vamvakaki M, Farsari M, Fotakis C, Chichkov BN. Ultra-Low Shrinkage Hybrid Photosensitive Material for Two-Photon Polymerization Microfabrication, ASC Nano, 2, 2257-2262; 2008.

Ovsianikov A, Chichkov BN. Three-dimensional Microfabrication by Two-photon Polymerization Technique, in: Computer-Aided Tissue Engineering, Liebschner, Michael; Sun, Wei (Eds.), Springer (in production).

Dinca V, Kasotakis E, Catherine J, Mourka A, Ranella A, Ovsianikov A, Chichkov BN, Farsari M, Mitraki A, Fotakis C. Directed Three-Dimensional Patterning of Self-Assembled Peptide Fibrils, Nanoletters, ASAP Article 10.1021/nl072798r, Nano Lett. 2008; 8, 2, 538-543.

Schlie S, Ngezahayo A, Ovsianikov A, Fabian T, Kolb HA, Haferkamp H, Chichkov BN. Three-dimensional cell growth on structures fabricated from ORMOCER® by two-photon polymerization technique. J Biomater Appl. 2007; 22, 275-87.

Ovsianikov A, Ostendorf A, Chichkov BN. Three-dimensional photofabrication with femtosecond lasers for applications in photonics and biomedicine. Applied Surface Science. 2007; 253: 15, 6599.

Ovsianikov A, Schlie S, Ngezahayo A, Haverich A, Chichkov BN. Two-photon polymerization technique for microfabrication of CAD designed three-dimensional scaffolds from commercially available photosensitive materials. J Tissue Eng Regen Med. 2007;1, 443-9.

Unit 32 - JRG Genetic &Epigenetic Integrity

C. Baum, U. Modlich, G. Gohring, B. Schlegelberger, Concise review: managing genotoxicity in the therapeutic modification of stem cells, Stem cells, 29 (2011) 1479-1484.

Q. Chen, X. Shi, C. Rudolph, Y. Yu, D. Zhang, X. Zhao, S. Mai, G. Wang, B. Schlegelberger, Q. Shi, Recurrent trisomy and Robertsonian translocation of chromosome 14 in murine iPS cell lines, Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 19 (2011) 857-868.

G. Gohring, A. Giagounidis, G. Busche, W. Hofmann, H.H. Kreipe, P. Fenaux, E. Hellstrom-Lindberg, B. Schlegelberger, Cytogenetic follow-up by karyotyping and fluorescence in situ hybridization: implications for monitoring patients with myelodysplastic syndrome and deletion 5q treated with lenalidomide, Haematologica, 96 (2011) 319-322.

G. Gohring, K. Lange, W. Hofmann, K.V. Nielsen, E. Hellstrom-Lindberg, L. Roy, M. Morgan, H. Kreipe, G. Busche, A. Giagounidis, B. Schlegelberger, Telomere shortening, clonal evolution and disease progression in myelodysplastic syndrome patients with 5q deletion treated with lenalidomide, Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, (2011).

G. Gohring, K. Michalova, H.B. Beverloo, D. Betts, J. Harbott, O.A. Haas, G. Kerndrup, L. Sainati, E. Bergstraesser, H. Hasle, J. Stary, M. Trebo, M.M. van den Heuvel-Eibrink, M. Zecca, E.R. van Wering, A. Fischer, P. Noellke, B. Strahm, F. Locatelli, C.M. Niemeyer, B. Schlegelberger, Complex karyotype newly defined: the strongest prognostic factor in advanced childhood myelodysplastic syndrome, Blood, 116 (2010) 3766-3769.

C. Lange, B. Brunswig-Spickenheier, H. Cappallo-Obermann, K. Eggert, U.M. Gehling, C. Rudolph, B. Schlegelberger, K. Cornils, J. Zustin, A.N. Spiess, A.R. Zander, Radiation rescue: mesenchymal stromal cells protect from lethal irradiation, PloS one, 6 (2011) e14486.

K. Lange, L. Holm, K. Vang Nielsen, A. Hahn, W. Hofmann, H. Kreipe, B. Schlegelberger, G. Gohring, Telomere shortening and chromosomal instability in myelodysplastic syndromes, Genes, chromosomes & cancer, 49 (2010) 260-269.

A. Osiak, F. Radecke, E. Guhl, S. Radecke, N. Dannemann, F. Luetge, S. Glage, C. Rudolph, T. Cantz, K. Schwarz, R. Heilbronn, T. Cathomen, Selection-independent generation of gene knockout in mouse embryonic stem cells using zinc-finger nucleases, PloS one, accepted (2011).

T. Ripperger, M. Tauscher, D. Haase, F. Griesinger, B. Schlegelberger, D. Steinemann, Managing individuals with propensity to myeloid malignancies due to germline RUNX1 deficiency, Haematologica, (2011).

T. Ripperger, M. Tauscher, I. Praulich, B. Pabst, A. Teigler-Schlegel, A. Yeoh, G. Gohring, B. Schlegelberger, C. Flotho, C.M. Niemeyer, D. Steinemann, Constitutional trisomy 8p11.21-q11.21 mosaicism: a germline alteration predisposing to myeloid leukaemia, British journal of haematology, 155 (2011) 209-217.

B. Schlegelberger, G.H. G, F. Thol, M. Heuser, Update on cytogenetic and molecular changes in myelodysplastic syndromes, Leukemia & lymphoma, (2011).

S. Stein, M.G. Ott, S. Schultze-Strasser, A. Jauch, B. Burwinkel, A. Kinner, M. Schmidt, A. Kramer, J. Schwable, H. Glimm, U. Koehl, C. Preiss, C. Ball, H. Martin, G. Gohring, K. Schwarzwaelder, W.K. Hofmann, K. Karakaya, S. Tchatchou, R. Yang, P. Reinecke, K. Kuhlcke, B. Schlegelberger, A.J. Thrasher, D. Hoelzer, R. Seger, C. von Kalle, M. Grez, Genomic instability and myelodysplasia with monosomy 7 consequent to EVI1 activation after gene therapy for chronic granulomatous disease, Nature medicine, 16 (2010) 198-204.

R. Tehranchi, P.S. Woll, K. Anderson, N. Buza-Vidas, T. Mizukami, A.J. Mead, I. Astrand-Grundstrom, B. Strombeck, A. Horvat, H. Ferry, R.S. Dhanda, R. Hast, T. Ryden, P. Vyas, G. Gohring, B. Schlegelberger, B. Johansson, E. Hellstrom-Lindberg, A. List, L. Nilsson, S.E. Jacobsen, Persistent malignant stem cells in del(5q) myelodysplasia in remission, The New England journal of medicine, 363 (2010) 1025-1037.

Nowak-Imialek M, Kues WA, Rudolph C, Schlegelberger B, Taylor U, Carnwath JW, Niemann H. Preferential loss of porcine chromosomes in reprogrammed inter-species cell hybrids. Cloning Stem Cells. 2009; accepted.

Rudolph C, Schlegelberger B. Spectral karyotyping and fluorescence in situ hybridization of murine cells. Methods Mol Biol. 2009; 506:453-66.

Koenigsmann J, Rudolph C, Sander S, Kershaw O, Gruber AD, Bullinger L, Schlegelberger B, Carstanjen D. NF1 haploinsufficiency and ICSBP-deficiency synergize in the development of leukemias. Blood. 2009;113:4690-701.

Christgen M, Bruchhardt H, Hadamitzky C, Rudolph C, Steinemann D, Gadzicki D, Hasemeier B, Römermann D, Focken T, Krech T, Ballmaier M, Schlegelberger B, Kreipe H, Lehmann U. Comprehensive genetic and functional characterization of IPH-926: a novel CDH1-null tumour cell line from human lobular breast cancer. J Pathol. 2008; J Pathol;217:620-32.

Modlich U, Schambach A, Brugman MH, Wicke DC, Knoess S, Li Z, Maetzig T, Rudolph C, Schlegelberger B, Baum C. Leukemia induction after a single retroviral vector insertion in Evi1 or Prdm16. Leukemia. 2008; 22:1519-28.

Templin C, Kotlarz D, Rathinam C, Rudolph C, Schätzlein S, Ramireddy K, Rudolph KL, Schlegelberger B, Klein C, Drexler H (2008). Establishment of immortalized multipotent hematopoietic progenitor cell lines by retroviral-mediated gene transfer of beta-catenin. Exp Hematol. 36:204-215.

Schaetzlein S, Kodandaramireddy NR, Ju Z, Lechel A, Stepczynska A, Lilli DR, Clark AB, Rudolph C, Wei K, Schlegelberger B, Schirmacher P, Kunkel TA, Greenberg RA, Edelmann W, Rudolph KL (2007): Exonuclease-1 deletion impairs DNA damage signaling and prolongs lifespan of telomere-dysfunctional mice. Cell. 130:863-877.

Meyer J, Rhein M, Schiedlmeier B, Kustikova O, Rudolph C, Kamino K, Neumann T, Yang M, Wahlers A, Fehse B, Reuther GW, Schlegelberger B, Ganser A, Baum C, Li Z (2007): Remarkable leukemogenic potency and quality of a constitutively active neurotrophin receptor, DeltaTrkA. Leukemia. 21:2171-2180.

Reimann M, Loddenkemper C, Rudolph C, Schildhauer I, Teichmann B, Stein H, Schlegelberger B, Dörken B, Schmitt CA (2007): The Myc-evoked DNA damage response accounts for treatment resistance in primary lymphomas in vivo. Blood. 110:2996-3004.

Heuser M, Argiropoulos B, Kuchenbauer F, Yung E, Piper J, Fung S, Schlenk RF, Dohner K, Hinrichsen T, Rudolph C, Schambach A, Baum C, Schlegelberger B, Dohner H, Ganser A, Humphries RK (2007): MN1 overexpression induces acute myeloid leukemia in mice and predicts ATRA resistance in AML patients. Blood. 110:1639-1647.

Ripperger T, von Neuhoff N, Kamphues K, Emura M, Lehmann U, Tauscher M, Schraders M, Groenen M, Skawran B, Rudolph C, Callet-Bauchu E, van Krieken JHJM, Schlegelberger B, Steinemann D (2007): Promoter methylation of PARG1, a novel candidate tumor suppressor gene in mantle cell lymphomas. Haematologica. 92:460-468.

Will E, Bailey J, Schuesler T, Modlich M, Balcik B, Burzynski B, Witte D, Layh-Schmitt G, Rudolph C, Schlegelberger B, von Kalle C, Baum C, Sorrentino BP, Wagner LM, Kelly, Lilith Reeves P, Williams DA (2007): Importance of murine study design for testing toxicity of retroviral vectors in support of Phase I trials. Mol Ther.15:782-791.

Unit 33 - SU Pathology of experimental animal models

D. Heckl, D.C. Wicke, M.H. Brugman, J. Meyer, A. Schambach, G. Busche, M. Ballmaier, C. Baum, U. Modlich, Lentiviral gene transfer regenerates hematopoietic stem cells in a mouse model for Mpl-deficient aplastic anemia, Blood, 117 (2011) 3737-3747.

C. Meyer-Schwesinger, C. Lange, V. Brocker, P.A. Agustian, U. Lehmann, A. Raabe, M. Brinkmeyer, E. Kobayashi, M. Schiffer, G. Busche, H.H. Kreipe, F. Thaiss, J.U. Becker, Bone marrow-derived progenitor cells do not contribute to podocyte turnover in the puromycin aminoglycoside and renal ablation models in rats, Am J Pathol, 178 (2011) 494-499.

M. Pincha, G. Salguero, D. Wedekind, B.S. Sundarasetty, A. Lin, N. Kasahara, M.H. Brugman, A.C. Jirmo, U. Modlich, R. Gutzmer, G. Busche, A. Ganser, R. Stripecke, Lentiviral vectors for induction of self-differentiation and conditional ablation of dendritic cells, Gene Ther, 18 (2011) 750-764.

D. Poehnert, V. Broecker, M. Mengel, B. Nashan, M. Koch, Induction of chronic renal allograft dysfunction in a rat model with complete and exclusive MHC incompatibility, Transpl Immunol, 22 (2010) 137-143.

D.C. Wicke, J. Meyer, G. Busche, D. Heckl, H. Kreipe, Z. Li, K.H. Welte, M. Ballmaier, C. Baum, U. Modlich, Gene therapy of MPL deficiency: challenging balance between leukemia and pancytopenia., Mol Ther., 18 (2010) 343-352.

T. Bisdas, M. Bredt, M. Pichlmaier, T. Aper, M. Wilhelmi, S. Bisdas, A. Haverich, O.E. Teebken, Eight-year experience with cryopreserved arterial homografts for the in situ reconstruction of abdominal aortic infections, J Vasc Surg, 52 (2010) 323-330.

M. Christgen, R. Geffers, M. Ballmaier, H. Christgen, J. Poczkaj, T. Krech, H. Kreipe, U. Lehmann, Down-regulation of the fetal stem cell factor SOX17 by H33342: a mechanism responsible for differential gene expression in breast cancer side population cells, J Biol Chem, 285 (2010) 6412-6418.

Singh AK, Riederer B, Krabbenhöft A, Rausch B, Bonhagen J, Lehmann U, de Jonge HR, Donowitz M, Yun C, Weinman EJ, Kocher O, Hogema BM, Seidler U. Differential roles of NHERF1, NHERF2, and PDZK1 in regulating CFTR-mediated intestinal anion secretion in mice. J Clin Invest. 2009 Mar;119(3):540-50.

Haase A, Olmer R, Schwanke K, Wunderlich S, Merkert S, Hess C, Zweigerdt R, Gruh I, Meyer J, Wagner S, Maier LS, Han DW, Glage S, Miller K, Fischer P, Schöler HR, Martin U. Generation of induced pluripotent stem cells from human cord blood. Cell Stem Cell. 2009 Oct 2;5(4):434-41.

Fischer K, Wolenski M, Manns MP, Jaeckel E. A new animal model of chronic autoimmune hepatitis mediated by the adaptive immune system. Paper presented at: 2008 EASL Monothematic Conference: Immune Mediated Liver Injury; Hamburg, Dec. 4-6, 2008

Warnecke G, Hutchinson JA, Riquelme P, Kruse B, Thissen S, Avsar M, Zehle G, Steinkamp T, Peters C, Baumann R, Gövert F, Ungefroren H, Länger F, Simon AR, Karstens JH, Kaever V, Haverich A, Fändrich F, Strüber M. Postoperative intravenous infusion of donor-derived transplant acceptance-inducing cells as an adjunct immunosuppressive therapy in a porcine pulmonary allograft model. Transpl Int. 2009 Mar;22(3):332-41. Epub 2008 Oct 21

Kruse B, Thissen S, Warnecke G, Avsar M, Gottlieb J, Hohlfeld JM, Karstens JH, Kaever V, Länger F, Pabst B, Ungefroren H, Haverich A, Strüber M. Correlation of donor leukocyte chimerism with pulmonary allograft survival after immunosuppressive drug withdrawal in a porcine model. Transplantation. 2009 May 27;87(10):1468-77.

Wiegmann B, Zardo P, Dickgreber N, Länger F, Fegbeutel C, Haverich A, Fischer S. Biological materials in chest wall reconstruction: initial experience with the Peri-Guard Repair Patch((R)). Eur J Cardiothorac Surg. 2010 Mar;37(3):602-5.

Schmitt R, Jacobi C, Susnik N, Broecker V, Haller H, Melk A. Ageing mouse kidney--not always the SAME old story. Nephrol Dial Transplant. 2009 Oct;24(10):3002-5.

Huber TB, Hartleben B, Winkelmann K, Schneider L, Becker JU, Leitges M, Walz G, Haller H, Schiffer M. Loss of podocyte aPKClambda/iota causes polarity defects and nephrotic syndrome. J Am Soc Nephrol. 2009 Apr;20(4):798-806. Epub 2009 Mar 11.

Ghosh A, Koestner W, Hapke M, Schlaphoff V, Langer F, Baumann R, Koenecke C, Cornberg M, Welte K, Blazar B, Sauer M. Donor T cells primed on leukemia lysate-pulsed recipient APCs mediate strong graft versus leukemia effects across MHC barriers in full chimeras. Blood. 2009 Apr 30;113(18):4440-8. Epub 2009 Jan 30.2009

Kocks JR, Adler H, Danzer H, Hoffmann K, Jonigk D, Lehmann U, Förster R. Chemokine receptor CCR7 contributes to a rapid and efficient clearance of lytic murine gamma-herpes virus 68 from the lung, whereas bronchus-associated lymphoid tissue harbors virus during latency. J Immunol. 2009 Jun 1;182(11):6861-9.

Nickel N, Kempf T, Tapken H, Tongers J, Laenger F, Lehmann U, Golpon H, Olsson K, Wilkins MR, Gibbs JS, Hoeper MM, Wollert KC. Growth differentiation factor-15 in idiopathic pulmonary arterial hypertension. Am J Respir Crit Care Med. 2008 Sep 1;178(5):534-41. Epub 2008 Jun 19.

Ghosh A, Koestner W, Hapke M, Schlaphoff V, Langer F, Baumann R, Koenecke C, Cornberg M, Welte K, Blazar B, Sauer M. Donor T cells primed on leukemia lysate-pulsed recipient APCs mediate strong graft versus leukemia effects across MHC barriers in full chimeras. Blood. 2009 Apr 30;113(18):4440-8. Epub 2009 Jan 30.2009

Kocks JR, Adler H, Danzer H, Hoffmann K, Jonigk D, Lehmann U, Förster R. Chemokine receptor CCR7 contributes to a rapid and efficient clearance of lytic murine gamma-herpes virus 68 from the lung, whereas bronchus-associated lymphoid tissue harbors virus during latency. J Immunol. 2009 Jun 1;182(11):6861-9

Hesse E, Kluge G, Atfi A, Correa D, Haasper C, Berding G, Shin HO, Viering J, Länger F, Vogt PM, Krettek C, Jagodzinski M. Repair of a segmental long bone defect in human by implantation of a novel multiple disc graft. Bone. 2010 Feb 12. [Epub ahead of print]

Nickel N, Kempf T, Tapken H, Tongers J, Laenger F, Lehmann U, Golpon H, Olsson K, Wilkins MR, Gibbs JS, Hoeper MM, Wollert KC. Growth differentiation factor-15 in idiopathic pulmonary arterial hypertension. Am J Respir Crit Care Med. 2008 Sep 1;178(5):534-41. Epub 2008 Jun 19.

Winter C, Taut K, Srivastava M, Länger F, Mack M, Briles DE, Paton JC, Maus R, Welte T, Gunn MD, Maus UA Lung-specific overexpression of CCL2 enhances the host defense to Streptococcus pneumoniae infection in mice: role of the CCL2-CCR2 axis. J Immunol, 2007 178: 5828-5838

Winter C, Taut K, Länger F, Mack M, Briles DE, Paton JC, Maus R, Srivastava M, Welte T, Maus UA FMS-like tyrosine kinase 3 ligand aggravates the lung inflammatory response to Streptococcus pneumoniae infection in mice: role of dendritic cells. J Immunol. 2007 179:3099-108

Mrigank Srivastava, Kathrin Steinwede, Riku Kiviranta, Jukka Morko, Heinz G Hoymann, Florian Laenger, Frank Buehling, Tobias Welte, Ulrich A Maus. Overexpression of cathepsin K in mice decreases collagen deposition and lung resistance in response to bleomycin-induced pulmonary fibrosis. Respiratory Research 2008, 9:54

Schreiber O, Steinwede K, Ding N, Srivastava M, Maus R, Länger F, Prokein J, Ehlers S, Welte T, Gunn MD, Maus UA. Mice that overexpress CC chemokine ligand 2 in their lungs show increased protective immunity to infection with Mycobacterium bovis bacille Calmette-Guérin. J Infect Dis 2008 198(7):1044-1054

Winter C, Herbold W, Maus R, Länger F, Briles DE, Paton JC, Welte T, Maus UA. Important role for CC chemokine ligand 2-dependent lung mononuclear phagocyte recruitment to inhibit sepsis in mice infected with Streptococcus pneumoniae. J Immunol. 2009 Apr 15;182(8):4931-7.

Nolff MC, Kokemueller H, Hauschild G, Fehr M, Bormann KH, Spalthoff S, Rohn K, Ruecker M, Gellrich NC. J Craniomaxillofac Surg. 2010 Jan;38(1):38-46. Epub 2009 Aug 22. Comparison of computed tomography and microradiography for graft evaluation after reconstruction of critical size bone defects using beta-tricalcium phosphate.

Kokemueller H, Spalthoff S, Nolff M, Tavassol F, Essig H, Stuehmer C, Bormann KH, Rücker M, Gellrich NC. Prefabrication of vascularized bioartificial bone grafts in vivo for segmental mandibular reconstruction: experimental pilot study in sheep and first clinical application. Int J Oral Maxillofac Surg. 2010 Feb 16. [Epub ahead of print]

Zhao F, Vermeer B, Lehmann U, Kreipe H, Manns MP, Korangy F, Greten TF. Identification of a novel murine pancreatic tumour antigen, which elicits antibody responses in patients with pancreatic carcinoma. Immunology. 2009 Sep;128(1):134-40

Wicke DC, Heckl D, Wintterle S, Buesche G, Meyer J, Kreipe H, Welte KH, Ballmaier M, Baum C, Modlich U. Ectopic expression of the extracellular domain of Mpl is sufficient to induce a hematopoietic population crisis. Submitted

Heckl D, Wicke DC, Schambach A, Büsche G, Meyer J, Kreipe H, Ballmaier M, Baum C, Modlich U. Correction of Mpl deficiency by lentiviral vectors with lineage-specific expression. Submitted

Heckl D, Wicke DC, Meyer J, Büsche G, Meyer J, Kreipe H, Baum C, Modlich U. Jak/Stat signal transduction and Ras activation are both essential for Mpl induced leukemogenicity. Submitted

Lenarz M, Lim HH, Lenarz T, Reich U, Marquardt N, Klingberg MN, Paasche G, Reuter G, Stan AC. Auditory midbrain implant: histomorphologic effects of long-term implantation and electric stimulation of a new deep brain stimulation array. Eur Arch Otorhinolaryngol. 2007 May;264(5):569-71

Wicke DC, Meyer J, Buesche G, Kreipe H, Li Z, Welte KH, Ballmaier M, Baum C, Modlich U. Gene therapy of mpl deficiency: challenging balance between leukemia and pancytopenia; submitted.

Scherr M, Dogan Y, Beutel G, Battmer K, Venturini L, Rudolph C, Büsche G, Schäfer D, Wilkens L, Schlegelberger B, Kreipe HH, Ganser A, Eder M: Stepwise ex vivo transformation of primary bone marrow progenitor cells to malignant phenotypes; submitted.

Haile LA, von Wasielewski R, Gamrekelashvili J, Krüger C, Bachmann O, Westendorf AM, Buer J, Liblau R, Manns MP, Korangy F, Greten TF. Myeloid-derived suppressor cells in inflammatory bowel disease: a new immunoregulatory pathway.Gastroenterology. 2008 Sep;135(3):871-81.

Schubert S, Kamino K, Böhm D, Adham I, Engel W, von Wasielewski R, Moharregh-Khiabani D, Mauceri G, Vaske B, Meinhardt A, Schöner A, Gonzalez-Fassrainer D, Schmidtke J. SPY expression is variably altered in transgenic mice with testicular feminization. Biol Reprod. 2008 Jul;79(1):125-33.

Lamlé J, Marhenke S, Borlak J, von Wasielewski R, Eriksson CJ, Geffers R, Manns MP, Yamamoto M, Vogel A. Nuclear factor-eythroid 2-related factor 2 prevents alcohol-induced fulminant liver injury. Gastroenterology. 2008 Apr;134(4):1159-68.

Metzelder M, Kuebler J, Shimotakahara A, Vieten G, von Wasielewski R, Ure BM. CO(2) pneumoperitoneum increases systemic but not local tumor spread after intraperitoneal murine neuroblastoma spillage in mice. Surg Endosc. 2008 Feb 13.

Ma B, Wang L, von Wasielewski R, Lindenmaier W, Dittmar KE. Serial sectioning and three-dimensional reconstruction of mouse Peyer's patch. Micron. 2008 Oct;39(7):967-75.

Bleich A, Sundberg JP, Smoczek A, von Wasielewski R, de Buhr MF, Janus LM, Julga G, Ukena SN, Hedrich HJ, Gunzer F. Sensitivity to Escherichia coli Nissle 1917 in mice is dependent on environment and genetic background. Int J Exp Pathol. 2008 Feb;89(1):45-54.

Ma B, von Wasielewski R, Lindenmaier W, Dittmar KE. Immmunohistochemical study of the blood and lymphatic vasculature and the innervation of mouse gut and gut-associated lymphoid tissue.Anat Histol Embryol. 2007 Feb;36(1):62-74.

Gamrekelashvili J, Krüger C, von Wasielewski R, Hoffmann M, Huster KM, Busch DH, Manns MP, Korangy F, Greten TF.Necrotic tumor cell death in vivo impairs tumor-specific immune responses.J Immunol. 2007 Feb 1;178(3):1573-80.

Christgen M, Ballmaier M, Bruchhardt H, von Wasielewski R, Kreipe H, Lehmann U. Identification of a distinct side population of cancer cells in the Cal-51 human breast carcinoma cell line. Mol Cell Biochem. 2007 Dec;306(1-2):201-12.

Büsche G, Hussein K, Bock O, Kreipe H. Insights into JAK2-V617F mutation in CML. Lancet Oncol. 2007 Oct;8(10):863-4.

Unit 34 - SU Molcular Toxicology

Rohrbeck, A. and Borlak, J. (2009) Cancer genomics identifies regulatory gene networks associated with the transition from dysplasia to advanced lung adenocarcinomas induced by c-Raf-1. PLoS One. 4:e7315.

Rohrbeck, A. Mueller V. and Borlak, J (2009) Molecular characterization of lung dysplasia induced by c-raf-. PLoS ONE. 4(5):e5637.

Falck, C. v., Rodt, T., Halter, R., Spanel, R., Galanski, M and Borlak, J (2009) Combined microPET/CT for imaging of hepatocellular carcinoma in mice. Frontiers in Bioscience. 14:2193-2202.

Rodt, T., Falck, C. v., Halter, R., Ringe, K., Shin, H.O., Galanski, M. and Borlak, J. (2009) In-vivo microCT quantification of lung tumor growth in SPC-raf transgenic mice. Frontiers in Bioscience. 14:1939-1944.

Rodt T, von Falck C, Halter R, Diensthuber M, Galanski M, Borlak J. (2009) Mikro-CT und 3D-Nachverarbeitung des Tracheobronchialsystems der Maus. 39. Kongress der Deutschen Gesellschaft für Endoskopie und Bildgebende Verfahren, München, Germany. Endoskopie heute 2009, 22: 65. (Lecture)

Rodt T, von Falck C, Halter R, Tillkorn C, Lotz J, Galanski M, Borlak J. (2009) Small animal imaging of lung cancer; comparison of different imaging modalities exemplified by SPC-myc and SPC-raf transgenic mouse models. European Congress of Radiology, Wien, Austria. European Radiology 2009, 19: Suppl. 1: S389

Falk, C. v., Rodt, T., Halter, R., Shin, H., Galanski, M. and Borlak, J. (2008) Die in-vivo microCT-Cholangiographie zur Evaluation des biliären Systems im murinen Tiermodell. 89. Deutscher Röntgenkongress. 5. Gemeinsamer Kongress der DRG und ÖRG, Berlin, Germany. RöFo - Fortschritte auf dem Gebiet der Röntgenstrahlen und der bildgebenden Verfahren 180, S1

Falk, C. v., Halter, R., Galanski, M. and Borlak, J. (2007) Morphologische und metabolische Phänotypisierung von transgenen Mausmodellen der Hepatokarzinogenese, 45. Jahrestagung der Deutschen Gesellschaft für Nuklearmedizin. Nuklearmedizin - Molecular Imaging and Therapy, 46: 49-64 (Abstract V184)

Unit 35 - SU Molcular Imaging and Marking

35a

Ibiricu, Huiskonen, Döhner, Bradke, Sodeik & Grünewald (2011). Cryo Electron Tomography of Herpes Simplex Virus during Axonal transport and Secondary envelopment in Neurons. PLoS Pathogens, accepted in October 2011.

Voelkel, Galla, Dannhauser, Mätzig, Sodeik, Schambach (corresponding) & Baum (corresponding) (2011). Pseudotype-independent non-specific uptake of gammaretroviral and lentiviral particles in human cells. Human Gene Therapy, accepted in October 2011.

Rode, Döhner, Binz, Glass, Strive, Bauerfeind & Sodeik (2011). Uncoupling uncoating of Herpes Simplex Virus genomes from their nuclear import and gene expression. Journal of Virology 85:4271-83 (Cover Image and Spotlight feature).

Radtke, Kieneke, Wolfstein, Michael, Steffen, Scholz, Karger & Sodeik (2010). Plus- and minus-end directed microtubule motors bind simultaneously to Herpes Simplex Virus capsids using different inner tegument structures. PLoS Pathogens 6:e1000991.doi10.1371/journal.ppat.1000991.

Zhang, Nagel, Sodeik & Lippe (2009). Early, active and specific localization of HSV1 gM to nuclear membranes. Journal of Virology, 83:12984-12997.

Roos (joint first author), Radtke (joint first author), Kniesmeijer, Geertsema, Sodeik (corresponding) & Wuite (corresponding) (2009). Scaffold expulsion and genome packaging trigger stabilization of Herpes Simplex Virus capsids. Proceedings of the National Academy of Sciences USA 106:9673-9678.

Jung, Ruthardt, Lewis, Michaelis, Sodeik, Nolde, Peneva, Müllen & Bräuchle (2009). Photophysics of new water-soluble terrylenediimide derivatives and applications in biology. ChemPhysChem 10:180-190.

Jirmo, Nagel, Bohnen, Sodeik & Behrens (2009). Contribution of direct and cross-presentation to CTL immunity against herpes simplex virus 1. Journal of Immunology 182:283-292.

Maurer, Sodeik & Grünewald (2008). Native 3D intermediates of membrane fusion in herpes simplex virus 1 entry. Proceedings of the National Academy of Sciences USA 105:10559-64. (Editors’ choice: Science 2008:321, page 892; Faculty of 1000 Biology)

Nagel, Döhner, Fathollahy, Strive, Borst, Messerle & Sodeik (2008). Nuclear egress and envelopment of herpes simplex virus capsids analyzed with dual-color fluorescence HSV1(17+). Journal of Virology 82:3109-24.

Borst, Wagner, Binz, Sodeik & Messerle (2008). The essential human cytomegalovirus gene UL25 is required for cleavage-packaging of the viral genome. Journal of Virology 82:2065-78.

Sodeik (2007). The cell entry of herpes simplex virus. Zellbiologie aktuell 33:10-13.

Döhner, Radtke, Schmidt & Sodeik (2006). The eclipse phase of Herpes Simplex Virus type 1 infection: Efficient dynein-mediated nuclear capsid targeting without the small capsid protein VP26. Journal of Virology 80:8211-24.

Köllner, Sodeik, Schreek, Heyn, von Neuhoff, Germeshausen, Zeidler, Krüger, Schlegelberger, Welte, Beger (2006). Mutations in neutrophil elastase causing congenital neutropenia lead to cytoplasmic protein accumulation and induction of the unfolded protein response. Blood 108:493-500.

Wolfstein, Nagel, Radtke, Döhner, Allan & Sodeik (2006). The inner tegument promotes herpes simplex virus capsid motility along microtubules in vitro. Traffic 7:227-237.

Radtke, Döhner & Sodeik (2006). Viral interactions with the cytoskeleton: a hitchhiker’s guide to the cell. Cellular Microbiology 8:387-400 (Invited Review).

35b

S. Turan, J. Bode, Review: Site-specific recombinases: from tag-and-target- to tag-and-exchange-based genomic modifications, The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, (2011).

Broll S, Oumard A, Hahn K, Schambach A & Bode J (2010) Minicircle Performance Depending on S/MAR-Nuclear Matrix Interactions. J. Mol.Biol. 395 (5), 950-965

S. Turan, M. Galla, E. Ernst, J. Qiao, C. Voelkel, B. Schiedlmeier, C. Zehe, J. Bode, Review: Recombinase-mediated cassette exchange (RMCE): traditional concepts and current callenges, J. Mol. Biol., 407 (2010) 193-221.

C. Voelkel, M. Galla, T. Maetzig, E. Warlich, J. Kuehle, D. Zychlinski, J. Bode, T. Cantz, A. Schambach, C. Baum, Protein transduction from retroviral Gag precursors, Proceedings of the National Academy of Sciences of the United States of America, 107 (2010) 7805-7810.

S. Turan, J. Kuehle, A. Schambach, C. Baum, J. Bode, Multiplexing RMCE: versatile extensions of the Flp-recombinase-mediated cassette-exchange technology, Journal of molecular biology, 402 (2010) 52-69.

Vidakovic M, Gluch A, Poznanovic G, Bode J. PARP-1 protein autoregulates its expression by binding to a recognition sequence and to an upstream S/MAR element in its own promoter. J. Mol. Biol. 2009; 388:730-50.

Qiao J, Oumard A, Wegloehner W, Bode J. Novel Tag-and-Exchange (RMCE) Strategies Generate Master Cell Clones with Predictable and Stable Transgene Expression Properties. J. Mol. Biol. 2009; 390:579-94.

Iqbal K, Barg-Kues B, Broll S, Bode J, Niemann H, Kues WA. Reprogramming events in early embryos are reflected by circular plasmid encoded marker genes. BioTechniques. 2009; in press. 

Voelkel, C., Galla, M., Maetzig, T., Warlich, E., Kuehle, J., Zychlinski, D., Bode, J., Schambach, A., Baum, C. (2009) Retroviral polyprotein-mediated transduction of Flp recombinase. 

Iqbal K, Barg-Kues B, Broll S., Bode. J., Niemann H, Kues WA (2009) Cytoplasmic injection of circular plasmids allows targeted expression in mammalian embryos. BioTechniques 47, 959–968

Qiao, J., Oumard, A., Wegloehner, W., Bode, J. (2009) Novel Tag-and-Exchange (RMCE) Strategies Generate Master Cell Clones with Predictable and Stable Transgene Expression Properties. J. Mol. Biol. 390, 579–594. doi:10.1016/j.jmb.2009.05.012

Zeitz MJ, Marella NRV, Malyavantham KS, Goetze S, Bode J, Raska I, Berezney R. (2009) Organization of the Amplified Type I Interferon Gene Cluster and Associated Chromosome Regions in the Interphase Nucleus of Human Osteosarcoma Cells., Chromosome Res.

Vidakovic M, Gluch A, Poznanovic G, Bode J. (2009) PARP-1 protein autoregulates its expression by binding to a recognition sequence and to an upstream S/MAR element in its own promoter,. 2009 Aug 15;107(6):1205-2.

Marella NRV, Zeitz MJ, Malyavantham KS, Pliss A, Matsui S, Goetze S, Bode J, Raska I & Berezney R. Organization of the Type I IFN Gene Cluster in the Human Osteosarcoma Cell Line MG63. Chromosoma, Chromosome Res. 2008, 16:1177–1192.

Unit 36 - SU MRT (Magnetic Resonance Tomography/Imaging)

Bankstahl J, Bankstahl M, Kuntner C, Stanek J, Wanek T, Meier M, Ding X, Müller M, Langer O, Löscher W, A Novel Positron Emission Tomography Imaging Protocol Identifies Seizure-Induced Regional Overactivity of P-Glycoprotein at the Blood-Brain Barrier, Journal of Neuroscience 31(24), 8803-8811, 2011

Kuntner C, Bankstahl JP, Bankstahl M, Stanek J, Wanek T, Stundner G, Karch R, Brauner R, Meier M, Ding X, Müller M, Löscher W, Langer O. Dose-response assessment of tariquidar and elacridar and regional quantification of P-glycoprotein inhibition at the rat blood-brain barrier using (R)-[(11)C]verapamil PET. Eur J Nucl Med Mol Imaging. 37(5):942-53. Epub 2009 Dec 17. PubMed PMID: 20016890, 2010

Meier M, Artifacts or Art in Fact: Useful susceptibility effects in MRI" in Biomagnetism: Interdisciplinary Research and Exploration, Suppl. 2008; ISBN 978-4-8329-0355-5.

Dreher W, Meier M, Erhard P, Leibfritz D. On the use of spin echo and stimulated echo based functional MRI sequences. Herrmann, M. & Thiel, C. (Eds.)(2007). Topics in Advanced Imaging. Oldenburg: bis-Publishers, 51-4.

Unit 37 - JRG Large Scale Cultivation

C. Bals, A. Schambach, J. Meyer, T. Scheper, U. Rinas, Expression and purification of bioactive soluble murine stem cell factor from recombinant Escherichia coli using thioredoxin as fusion partner, J Biotechnol, 152 (2011) 1-8.

R. Chen, J. John, A. Lavrentieva, S. Müller, M. Tomala, Y. Zhao, R. Zweigerdt, S. Beutel, B. Hitzmann, C. Kasper, U. Martin, U. Rinas, F. Stahl, T. Scheper, Cytokine production using membrane adsorbers: Human basic fibroblast growth factor produced by Escherichia coli., Eng. Life Sci., 12 (2012) 1-10.

R. Chen, J. John, B. Rode, B. Hitzmann, R. Gerardy-Schahn, C. Kasper, T. Scheper, Comparison of polysialic acid production in Escherichia coli K1 during batch cultivation and fed-batch cultivation applying two different control strategies, J Biotechnol, 154 (2011) 222-229.

A. Christel, S. Williams, C. Kasper, A. Hoffmann, P. Behrens, Nanoparticles for multiple functionalization of scaffolds for stem cell engineering, Tissue Engineering Part A, 17 (2011) 564-565.

R. Hass, C. Kasper, S. Bohm, R. Jacobs, Different populations and sources of human mesenchymal stem cells (MSC): A comparison of adult and neonatal tissue-derived MSC, Cell Commun Signal, 9 (2011) 12.

T. Hatlapatka, C. Kasper, A. Lavrentieva, R. Hass, N. Marquardt, R. Jacobs, Shaping the Microenvironment of Umbilical Cord-derived Mesenchymal Stem Cell-like Cells., Tissue Engineering Part A, 17 (2011) 575.

T. Hatlapatka, P. Moretti, A. Lavrentieva, R. Hass, N. Marquardt, R. Jacobs, C. Kasper, Optimization of culture conditions for the expansion of umbilical cord-derived mesenchymal stem or stromal cell-like cells using xeno-free culture conditions, Tissue Eng Part C Methods, 17 (2011) 485-493.

A. Hilfiker, C. Kasper, R. Hass, A. Haverich, Mesenchymal stem cells and progenitor cells in connective tissue engineering and regenerative medicine: is there a future for transplantation?, Langenbecks Arch Surg, 396 (2011) 489-497.

J.W. Kuhbier, K. Reimers, C. Kasper, C. Allmeling, A. Hillmer, B. Menger, P.M. Vogt, C. Radtke, First investigation of spider silk as a braided microsurgical suture, J Biomed Mater Res B Appl Biomater, 97 (2011) 381-387.

I. Majore, P. Moretti, F. Stahl, R. Hass, C. Kasper, Growth and differentiation properties of mesenchymal stromal cell populations derived from whole human umbilical cord, Stem Cell Rev, 7 (2011) 17-31.

P. Moretti, T. Hatlapatka, D. Marten, A. Lavrentieva, I. Majore, R. Hass, C. Kasper, Mesenchymal stromal cells derived from human umbilical cord tissues: primitive cells with potential for clinical and tissue engineering applications, Adv Biochem Eng Biotechnol, 123 (2010) 29-54.

K. Ralla, U. Sohling, K. Suck, F. Sander, C. Kasper, F. Ruf, T. Scheper, Adsorption and separation of proteins by a synthetic hydrotalcite, Colloids Surf B Biointerfaces, 87 (2011) 217-225.

F. Stahl, B. Hitzmann, K. Mutz, D. Landgrebe, M. Lubbecke, C. Kasper, J. Walter, T. Scheper, Transcriptome Analysis, Adv Biochem Eng Biotechnol, (2011).

K. Suck, S. Roeker, S. Diederichs, F. Anton, J.A. Sanz-Herrera, I. Ochoa, M. Doblare, T. Scheper, M. van Griensven, C. Kasper, A rotating bed system bioreactor enables cultivation of primary osteoblasts on well-characterized Sponceram regarding structural and flow properties, Biotechnol Prog, 26 (2010) 671-678.

H. Wendt, A. Hillmer, K. Reimers, J.W. Kuhbier, F. Schafer-Nolte, C. Allmeling, C. Kasper, P.M. Vogt, Artificial skin--culturing of different skin cell lines for generating an artificial skin substitute on cross-weaved spider silk fibres, PLoS One, 6 (2011) e21833.

Tomala M, Lavrentieva A, Moretti P, Rinas U, Kasper C, Stahl F, Schambach A, Wahrlich E, Martin U, Cantz T, Scheper T, Preparation of bioactive soluble human leukemia inhibitory factor from recombinant Escherichia coli using thioredoxin as fusion partner. Protein Expr Purif. 2010 Apr 8. [Epub ahead of print]

Kuhbier JW, Weyand B, Radtke C, Vogt PM, Kasper C, Reimers K, Isolation, Characterization, Differentiation, and Application of Adipose-Derived Stem Cells. Adv Biochem Eng Biotechnol. 2010 Jan 21. [Epub ahead of print]

M. van Griensven , S. Diederichs , S. Roeker , S. Boehm , A. Peterbauer, S. Wolbank , D. Riechers , F. Stahl , and C. Kasper, Mechanical Strain Using 2D and 3D Bioreactors Induces Osteogenesis: Implications for Bone Tissue Engineering. Adv Biochem Engin/Biotechnol (2009) 112: 95–123

Solvig Diederichs, Stefanie Roeker, Dana Marten, Anja Peterbauer, Thomas Scheper, Martijn van Griensven, Cornelia Kasper Biotechnol, Dynamic Cultivation of Human Mesenchymal Stem Cells in a Rotating Bed Bioreactor System Based on the ZRP Platform. Prog., 2009, Vol. 25, No. 6

Majore I, Moretti P, Hass R, Kasper C. Identification of subpopulations in mesenchymal stem cell-like cultures from human umbilical cord. Cell Commun Signal. 2009;7:6.

Ralla K, Anton F, Scheper T, Kasper C. Application of conjoint liquid chromatography with monolithic disks for the simultaneous determination of immunoglobulin G and other proteins present in a cell culture medium. Journal of Chromatography A. 2009;1216(13):2671-75.

Rober M, Walter J, Vlakh E, Stahl F, Kasper C, Tennikova T. New 3 D microarray platform based on macroporous polymer monoliths. Analytica Chimica Acta. 2009;644, 95.

Roeker S, Diederichs S, Peterbauer A, Scheper T, van Griensven M, Kasper C. Dynamic cultivation of human mesenchymal stem cells in a rotating bed bioreactor system based on the ZRP platform. Biotechnology Progress (accepted for publication).

Roeker S, Boehm S, Diederichs S, Bode F, Quade A, Korzhikov V, van Griensven M, Tennikova TB, Kasper C. A study on the influence of biocompatible composites with bioactive ligands toward their effect on cell adhesion and growth for the application in bone tissue engineering. J Biomed Mater Res B Appl Biomater. 2009; 91B(1-2), 153.

Suck K, Behr L, Fischer M, Hofmeister H, van Griensven M, Stahl F, Scheper T, Kasper C. Cultivating preosteoblastic MC3T3-E1 cells on a newly developed material (Sponceram®) using a rotating bed system (RBS) bioreactor; J. Biomed. Mat.. Res. 80A, 2, 2006, 268.

Bruns S, Stark Y, Wieland M, Stahl F, Kasper C, Scheper T. Fast and efficient screening systems for new biomaterials in tissue engineering: a model for peripheral nerve regeneration; J. Biomed. Mat.. Res. A, 3, 2007, 736.

Stark Y, Bruns S, Stahl F, Kasper C, Grothe C, Wesemann M, Scheper T. Polysialic acid as material for cell cultivation; J. Biomed. Mat. Res. A 85A, 2008, 1.

Korzikov V, Roeker S, Vlakh E, Kasper C, Tennikova T. Synthesis of multifunctional polyvinylsaccaride containing controllable amounts of biospecific ligands; Bioconjugate Chem. 19 (3), 2008, 617.

Rode B, Endres C, Ran C, Stahl F, Beutel S, Kasper C, Galuska S, Geyer R, Mühlenhoff M, Gerardy-Schahn R, Scheper T. Large-scale production and homogenous purification of long chain polysialic acids from E. coli K1; J. Biotechnol. 135 (2), 2008, 202.

Unit 38 - SU Biocompatibility

Hahn, J. Fuhlrott, A. Loos, S. Barcikowski, Cytotoxicity and ion release of alloy nanoparticles, Journal of Nanoparticle Research, 14 (2012) 1-10.

Fuhlrott J.; Loos A.; Glasmacher B.; Löbler M.: Endozytose und Zytotoxizität von Nanopartikeln. BIOmaterialien, 2009; 10 (3/4): 109.

Ghodsizad A, Niehaus M, Kögler G, Martin U, Wernet P, Bara C, Khaladj N, Loos A, Makoui M, Thiele J, Mengel M, Karck M, Klein HM, Haverich A, Ruhparwar A. Transplanted human cord blood-derived unrestricted somatic stem cells improve left-ventricular function and prevent left-ventricular dilation and scar formation after acute myocardial infarction. Heart. 2009 Jan;95(1):27-35.

Loos A., Rohde R, Haverich A, Barlach S. In Vitro and In Vivo Biocompatibility Testing of Absorbable Metal Stents. Macromolecular Symposia, 2007; 253 (1) 103-8.

Waksman R, Pakala R, Kuchulakanti PK, Baffour R, Hellinga D, Seabron R, Tio FO, Wittchow E, Hartwig S, Harder C, Rohde R, Heublein B, Andreae A, Waldmann KH, Haverich A. Catheter Cardiovasc Interv. 2006; 68(4):607-17; discussion 618-9.

Heublein B, Rohde R, Kaese V, Niemeyer M, Hartung W, Haverich A. Heart. 2003; 89(6):651-6.

Heublein B, Evagorou EG, Rohde R, Ohse S, Meliss RR, Barlach S, Haverich A. Int J Artif Organs. 2002; 25(12):1166-73.

Unit 39 - SU GXP

Gama-Norton, L., Botezatu, L., Herrmann, S., Schweizer, M., Alves, P.M., Hauser, H., Wirth.D. Lentivirus production is influenced by SV40 large T Antigen and chromosomal integration of the vector in HEK293 cells (2011) Hum. Gene Ther. In press

Alkharsah, K., Singh, V.V., Bosco, R., Santag, S., Grundhoff, A., Konrad, A., Stürzl, M., Wirth, D., Dittrich-Breiholz, O., Kracht, M., Schulz, T. F. (2011). Deletion of Kaposi’s sarcoma herpesvirus FLICE inhibitory protein (vFLIP) from the viral genome compromises the activation of STAT-1 responsive cellular genes and spindle cell formation in endothelial cells. J Virol 85(19):10375-88.

L. Botezatu, S.  Sievers, L. Gama-Norton, R. Schucht, H. Hauser, D. Wirth. Genetic aspects of cell line development from a synthetic biology perspective. Adv. Biochem. Eng. Biotechnol., in press

Loew, R., Meyer M., Kuehlcke. K., Gama-Norton, L., Wirth, D.,Hauser, H., Stein., S., Grez., M. Thornhill, S., Thrasher, A., Baum, C., and Schambach, A. (2010) A new PG13-based packaging cell line for stable production of clinical grade self inactivating g-retroviral vectors using targeted integration. Gene Therapy, 17, 272-280.

Nehlsen, K., Schucht, R., da Gama-Norton, L., Kromer, W., Baer, A., Cayli, A., Hauser, H. and Wirth, D. (2009) Recombinant protein expression by targeting pre-selected chromosomal loci. BMC Biotechnol, 9, 100.

Schucht, R., Wirth, D. and May, T. (2010) Precise regulation of transgene expression level and control of cell physiology. Cell Biol Toxicol, 26, 29-42.

Gama-Norton, L., Herrmann, S., Schucht, R., Coroadinha, A., Löw, R., Alves, P., Baum, C., Schambach, A., Hauser, H., and Wirth, D.  Retroviral vector performance upon integration into defined chromosomal loci of modular packaging cell lines. Hum. Gene Ther. 2010 21(8):979-91.

Surdziel E, Cabanski M, Dallmann I, Lyszkiewicz M, Krueger A, Ganser A, Scherr M, Eder M (2011). Enforced expression of miR-125b affects myelopoiesis by targeting multiple signalling pathways. Blood, 117(16):4338-4348.

Schucht, R., Lyford, S., Andzinski, L., Zauers, J., Cooper, J., Hauser, H., Wirth, D., May, T. Rapid establishment of GPCR expressing cell lines by site specific integration. J. Biomol. Screening, 16(3):323-331

Carrondo, M., Panet, M., Wirth, D., Coroadinha, AS, Cruz, P., Falk, H., Schucht, R., Dupont, F., Geny-Fiamma, C., Merten, O.-W., Hauser, H. Integrated strategy for the production of therapeutic retroviral vectors. Hum Gene Ther., 22(3):370-9.

Sandhu, U., Cebula, M., Behme, S., Riemer, P., Wodarczyk, C., Reimann, J., Schirmbeck, R., Hauser, H. and Wirth, D. Strict control of transgene expression expression in a mouse model for sensitive biological applications. (2011) Nucleic Acids Res. 39, e1

Surdziel E, Eder M, Scherr M. Lentivirus-mediated antagomir expression. (2010) Methods Mol Biol. ‘MicroRNAs and the Immune System’ , chapter 4.1

Lindenmaier,W., Lachmann,K., Meyring,W., Garritsen,H., Thomas,M., and Dittmar,K.E.J. (2011). Innenbeschichtung von Beuteln für die Kultur adhärenter humaner Zellen. BIOspektrum 17, 32-34.

Shahab-Osterloh,S., Witte,F., Hoffmann,A., Winkel,A., Laggies,S., Neumann,B., Seiffart,V., Lindenmaier,W., Gruber,A.D., Ringe,J., Haupl,T., Thorey,F., Willbold,E., Corbeau,P., and Gross,G. (2010). Mesenchymal stem cell-dependent formation of heterotopic tendon-bone insertions (osteotendinous junctions). Stem Cells. 28, 1590-1601.

Macke,L., Garritsen,H.S., Meyring,W., Hannig,H., Pägelow,U., Wörmann,B., Piechaczek,C., Geffers,R., Rohde,M., Lindenmaier,W., and Dittmar,K.E. (2010). Evaluating maturation and genetic modification of human dendritic cells in a new polyolefin cell culture bag system. Transfusion. 50, 843-855.

Lachmann, K., Dohse, A., Zghoul, N., Thomas, M., Dittmar, K. E. J., Lindenmaier, W., and Klages, C.-P. (2009) Tailor-made surface coatings for cell cultivation in a closed plastic bag system. ISPC-Proceedings.2009, epub.

Garritsen,H.S., Macke,L., Meyring,W., Hannig,H., Pägelow,U., Wormann,B., Geffers,R., Dittmar,K.E., and Lindenmaier,W. (2010). Efficient generation of clinical-grade genetically modified dendritic cells for presentation of multiple tumor-associated proteins. Transfusion. 50, 831-842.

Dittmar,K.E.J., Simann,M., Zghoul,N., Schön,O., Meyring,W., Hannig,H., Macke,L., Dirks,W., Miller,K., Garritsen,H., and Lindenmaier,W. (2010). Quality of cell products: authenticity, identity, genomic stability and status of differentiation. Transfusion Medicine and Hemotherapy 37, 57-64.

Nehlsen, K., Herrmann, S., Zauers, J., Hauser, H. and Wirth, D. (2010) Toxin-antitoxin based transgene expression in mammalian cells. Nucleic Acids Res. 38:e32

May, T., Butueva, M., Hauser, H. and Wirth, D. (2010) Synthetic gene regulation circuits for control of cell expansion. Tissue Engineering,  16, 441-452

Dogan Y, Ganser A, Scherr M, Eder M. (2010) Quantification of transforming capacity and cooperation of defined genetic alterations in myeloid malignancies. Exp Hematol. 38:11-9.

Wirth D, Gamma Norton L, Riemer P, Sandhu U, Schucht R, and Hauser H. (2007) Road to precision: recombinase based targeting technologies for genome engineering. Curr. Opin. Biotech. 18, 411-419.

May T, Eccleston L, Herrmann S, Hauser H, Goncalves J, Wirth D. (2008) Bimodal and hysteretic expression in mammalian cells from a synthetic gene circuit. ) PlosOne 3(6):e2372.

Ma B, Lin Z, Winkelbach S, Lindenmaier W, Dittmar KE. Automatic registration of serial sections of mouse lymph node by using Image-Reg. Micron. 39 (4):387-396, 2008.

Ma B, Zimmermann T, Rohde M, Winkelbach S, He F, Lindenmaier W, Dittmar KE. Use of Autostitch for automatic stitching of microscope images. Micron. 2007; 38: 492-499

Ma B, Jablonska J, Lindenmaier W, Dittmar K.E. Immunohistochemical study of the reticular and vascular network of mouse lymph node using vibratome sections. Acta Histochem. 2007; 109: 15-28.

Ma B, He F, Jablonska J, Winkelbach S, Lindenmaier W, Zeng AP, Dittmar K.E.J. Six-color segmentation of multicolor images in the infection studies of Listeria monocytogenes. Microscopy Research and Technique 2007; 70: 171-178.

Klopper J, Lindenmaier W, Fiedler U, Mehlhorn A, Stark GB, Finkenzeller G. High efficient adenoviral-mediated VEGF and Ang-1 gene delivery into osteogenically differentiated human mesenchymal stem cells. Microvasc.Res. 2008; 75: 83-90.

Ma B, Wang L, von Wasielewski R, Lindenmaier W, Dittmar KEJ. Serial sectioning and three-dimensional reconstruction of mouse Peyer's patch. Micron. 39 (7):967-975, 2008.

Ma B, von Wasielewski R, Lindenmaier W, Dittmar K.E.J. Immunohistochemical study of the blood and lymphatic vasculature and the innervation of mouse gut and gut-associated lymphoid tissue. Anat.Histol.Embryol. 2007; 36: 62-74.1.

Lohmeyer, J. A., Liu, F., Krüger, S., Lindenmaier, W., Siemers, F., and Machens, H. G. Use of gene-modified keratinocytes and fibroblasts to enhance regeneration in a full skin defect. Langenbeck's Archives of Surgery . 2011; 396:543-50

Spanholtz T, Maichle A, Niedworok C, Stoeckelhuber BM, Kruger S, Wedel T, Aach T, Middeler G, Hellwig-Burgel T, Bader A, Krengel S, Muller OJ, Franz WM, Lindenmaier W, Machens HG. Timing and Targeting of Cell-based VEGF(165) Gene Expression in Ischemic Tissue. J.Surg.Res., 2008,151:153-162.

May T, Hauser H, Wirth D. In vitro expansion of tissue cells by conditional proliferation. Methods in Molecular Biology. 2007;140:1-15.

Scherr M, Venturini L, Eder M. Knock-down of gene expression in hematopoietic cells. Methods Mol Biol. 2009;506:207-19.

Scherr M, Venturini L, Battmer K, Schaller-Schoenitz M, Schaefer D, Dallmann I, Ganser A, Eder M. Lentivirus-mediated antagomir expression for specific inhibition of miRNA function. Nucleic Acids Res. 2007;35(22):e149. Epub 2007 Nov 19.

May T, Hauser H. and Wirth D. (2007) In vitro expansion of tissue cells by conditional proliferation. Methods in Molecular Biology;140:1-15

Unit 40 - Hannover Clinical Trial Center

C. Walter, B. Rohde, D.C. Wicke, C. Pohler, A. Luehrmann, H. von der Leyen, Klinische Entwicklung neuartiger Therapien: Vom Labor zur klinischen Prüfung., Bundesgesundheitsblatt, 54 (2011) 803-810.

C. Voelkel, A. Luehrmann, C. Baum, H.E. von der Leyen, Retrovirus mediated hematopoietic gene therapy: A European regulatory perspective with special focus on the situation in Gemany., www.ctt-journal.com/1-4-en-voelkel-et-al-2009may.html, (2009).

H. von der Leyen, C.L. Voelkel, Hannover Clinical Trial Center - Zentrale Schnittstelle für das Management innovativer klinischer Forschung, Biospektrum, 7 (2007).