General Information

Video

Unit 5 - RG Cell Surgery (belongs to AG 27 - W2 Biophotonics)

Objectives

The aims of the RG Cell Surgery and the professorship "Biophotonics" are the study and development of optical tools for the manipulation of cells and cellular functions. Optical techniques allow the achievement of highly localized effects within cells, which can be simultaneously used for high resolution imaging.

Research Focus

The aims of the RG Cell Surgery and the professorship "Biophotonics" are the study and development of optical tools for imaging and manipulation of biological structures. Using nonlinear imaging modalities, like multiphoton microscopy and second- or third-harmonic generation, tissue slices up to 1 mm-thickness can be imaged non-invasively. For three-dimensional visualization of centimeter-sized biological objects, tomographic methods are developed and evaluated.  By using further nonlinear effects, precise manipulation or ablation of cells or tissue structures can be achieved, for example for cell enucleation or cell transfection. To enable high-throughput processing, plasmonic effects in noble nanoparticles, such as gold nanoparticles (GNP) or gold nanorods, are being studied as potential markers for higher contrast or selective therapy.

Collaborations

• Prof. H. Niemann, Institute of Farm Animal Genetics Mariensee, REBIRTH, RG Reprogramming, somatic cloning, sperm sexing (additional funding acquired in 2008)
• Prof. B. Bullerdiek, Prof. I. Nolte, University of Veterinary Medicine Hannover, REBIRTH, W2 Biomaterials, laser transfection of stem cells (additional funding by TR37 in 2007) 
• Dr. S. Barcikowski, Laser Zentrum Hannover, REBIRTH, JRG Nanoparticles, sperm sexing (together with Niemann), plasmonic imaging and cell manipulation 
• Dr. I. Gruh, Hannover Medical School, REBIRTH, JRG Myocardial Tissue Engineering, collagen cross-linking in bioartificial cardiac tissue
• Dr. T. Aper, Hannover Medical School, LEBAO, imaging and quality control of tissue engineered vascular grafts 
• Prof. W. Wolkers, Prof. B. Glasmacher, Leibniz University Hannover, REBIRTH, W3 Biomedical Process Technology, imaging and analysis/test of processed heart valves (homografts), together with Dr. N. Hofmann, Leibniz Universität Hannover, REBIRTH, JRG Biothermodynamics
• Prof. B. Sodeik, Hannover Medical School, REBIRTH, SU Molecular Imaging, laser transfection of primary cell lines 
• Prof. E. Mazur, Harvard University; Prof. J. C. Bischof, University of Minnesota
• In joint projects: Prof. K. Liefeith, IBA Bad Heiligenstadt; Prof. U. Morgner, Leibniz University Hannover; Prof. R. Guthoff, University of Rostock; Prof. C. Junghanss, University Rostock, Medical Clinic; Prof. D. Rath, FLI Mariensee; Prof. R. Lenarz, Hannover Medical School; Prof. Möller, RWTH Aachen; Prof. M. Stiesch, Hannover Medical School, Clinic for Dental Prosthetics; Dr. A. Winkel, Hannover Medical School, Clinic for Dental Prosthetics; Prof. M. Ochs, Hannover Medical School, Institute for Functional and Applied Anatomy; Dr. M.-P. Kühnel, Hannover Medical School, Institute for Functional and Applied Anatomy; Prof. G. Reuter, Hannover Medical School, Clinic for Laryngology, Rhinology and Otology; Dr. Robert Zweigerdt, LEBAO

Further Research Projects

• Principal investigator in the crosssectional project Q1, Transregio 37, Cellular Laser Microscopy
• Project A2, Transregio 37, Laser Transfection of Haematopoetic Stem Cells 
• Collaborative project Nanotome, funded by the BMBF 
• Various industry projects related to cell characterisation and cell sorting

Awards

• Scientific award in the startup-competition of HannoverImpuls (2009)

Teaching

• Lectureship in the physic courses at Leibniz University (Lasermedicine, Biophotonics, etc.), lectureship in HBRS, lectureship in biomedical engineering courses.

Equipment and Service Facilities

[Please find a list of all available equipment here]

Publications

[Please find the publications of this workgroup here]

Further Information

www.biophotonics.uni-hannover.de

Staff