REBIRTH Members

Bode, Jürgen

Bode, Jürgen, Prof. Dr. (PhD)

Experimental Hematology

Hannover Medical School
Carl-Neuberg-Str. 1
30625 Hannover

Bode Juergen@mh-hannover.de

Biography / About

Date of Birth:March, 1944
Function:Honorary Professor in Hannover Medical School(MHH) since April 2009

Education:

1963Higher secondary education (Abitur), Neue Oberschule, Braunschweig
1969Diploma (MSc) in Chemistry, Technical University of Braunschweig, FRG
1969 - 1971PhD in Chemistry, Technical University of Braunschweig, FRG
1971 - 1973Research Associate, California Institute of Technology, Pasdena
1974Research Associate University of Oregon, Eugene
Fellowships1971-1974 DFG Career-Development Grant

Academic appointments and Research posts:

1981Post-doctoral lecturing qualification (Habilitation) (Biochemistry) Technical University of Braunschweig
1987Professor (APL) Technical University of Braunschweig
1993Visiting Professor Hadassah Medical School, Jerusalem
1998 - 2001Project Leader “Vectors for Gene Therapy”, GBF
1999 - 2009Group Leader “Epigenetic Regulation” GBF
2009Professor emeritus at MHH (REBIRTH/SFB-subgroups in the Department of Experimental Hematology initiated by C. Baum)

Other professional activities:

1994 - 2009Chairman “Arbeitskreis für Zellbiologie und Biomedizinische Forschung e.V., Braunschweig”
1983 - 2009Leading Irradiation Protection Officer (GBF/HZI)
1996 - 2004GBF Advisory Board (Aufsichtsrat)
Since 1996 Editorial Boards: - „Molecular Biology Reports“
Since 1998 - “Gene Therapy and Molecular Biology”
Since 2007- “PLoS Open Access Journal”
Since 1997Advisory Board Member Regulon Inc.
Since 1999Advisory Board Member Genomatix (Munich)
Since 2001Advisory Board Member ProBioGen (Berlin)
2009 - 2010Advisor CellCA/Rentschler (Laupheim)
Since 2009Int. Society for Gene Therapy and Molecular Biology; ESGCT

Reviewer for DFG, DAAD, Alexander von Humboldt Stiftung, Studienstiftung des Deutschen Volkes, INTAS, NIH

Awards and Prizes:

1971Ph.D. awarded summa cum laude, Technical University of Braunschweig
-DFG Research Awardee (6x); EU (2x); NATO; INTAS

Major research interests:

Structure and function of the eukaryotic nucleus with regard to gene expression

  1. Methods for the systematic modification of the eukaryotic nucleus: transgenic cell lines and –animals with predictable expression characteristics

    1. Development and application of Flp-mediated cassette exchange techniques (RMCE) since 1996 (REBIRTH project 2007-2012);
    2. Development and application of nonviral circular replicating episomes for non-destructive transgenesis since 1999 (FW6-project until 2008; SFB until 2012)

  2. Analysis and design of autonomously regulated transcription units (Chromatin domains)

    1. Chromosome-based vectors
    2. Systems Biology approaches (SIDD) towards and in situ verification of chromatin domains (cooperation with the Genome Center at UC Davis, CA)
    3. Analysis of type I interferon genes in various mammals: domain structure and (co-) regulation of a prototype gene cluster;

  3. Structure and function of retroviral integration sites; relation to nuclear structure
  4. Function of genomic insulators and S/MARs Options for the generation of autonomously regulated expression units by

    • either site-specific integration (RMCE; References 1,3,9)
    • or noviral replicating episomes (“minicircles”; References 2,4,5)

Furthermore:

Supervision of Students and Teaching obligations
Since my habilitation, 1981, I have continuously supervised doctoral candidates for Cell Biology and Biochemistry. Among the 94 PhD procedures l guided ot the TU Braunschweig, mostly on behalf of GBF, there were 22 students that performed their thesis under my direct leadership.

Until WS 2008 I have been in charge of the principal lectures and courses in Biochemistry [lectures "Introduction to Biochemistry 1 and -2", basic practical course in biochemistry and enzyme kinetics].

Selected Publications:

  1. Turan S, Qiao J, Madden S, Benham C, Kotz M, Schambach A, Bode J Expanding Flp-RMCE options: The potential of Recombinase Mediated Twin-site Targeting (RMTT). Gene 2014; 546, 135-144 dx.doi.org/10.1016/j.gene.2014.06.002
  2. Kymäläinen H, Davies A, Ogilvie C, Appelt JU, Giordan F, Laufs S, Schmidt M, Bode J, Yáñez-Muñoz RJ & Dickson G. Long-term episomal transgene expression from mitotically stable integration-deficient lentiviral vectors (IDLVs). Hum. Gene Ther. Vol. 2014; 25, (5) 428-442 dx.doi.org/10.1089/hum.2013.172
  3. Turan S, Kuehle J, Schambach A, Baum, C & Bode J (2010) Multiplexing RMCE: Versatile Extensions of the Flp-Recombinase-Mediated Cassette-Exchange Technology J. Mol. Biol. 2010; 402 52-69 dx.doi.org/10.1016/j.jmb.2010.07.015
  4. Kues W, Niemann H., Iqbal K, Garrels W, Bode J. Nonviral episomes permit targeted expression in mammalian embryos Rebirth News 2009; 03: 6-7. elm-asse-kultur.de/PDF/6_pdfsam_.pdf
  5. Nehlsen K, Broll S Bode J (2006) Replicating minicircles: Generation of nonviral episomes for the efficient modification of dividing cells. Gene Ther. Mol. Biol. 2006; 10:233-244. www.gtmb.org/volumes/Vol10/25.%20Nehlsen%20et%20al,%20233-244.pdf
  6. Bode J, Winkelmann S, Goetze S, Spiker S, Tsutsui K, Bi C, AK P, Benham C Correlations between scaffold/matrix attachment region (S/MAR) binding activity and DNA duplex destabilization energy. J. Mol. Biol. 2006; 358:597-613
  7. Bode J, Goetze S, Heng H, Krawetz SA, Benham C From DNA structure to gene expression: mediators of nuclear compartmentalization and dynamics. Chromosome Res. 2003; 11:435-445. dx.doi.org/10.1023/A:1024918525818
  8. Bode J, Benham C, Knopp A, Mielke C Transcriptional augmentation: modulation of gene expression by scaffold/matrix attached Regions (S/MAR Elements). Crit. Rev. Eukaryot. Gene Expr. 2000; 10:73-90. www.ncbi.nlm.nih.gov/pubmed/10813396
  9. Schlake T & Bode J (1994) Use of mutated FLP-recognition-target-(FRT-)sites for the exchange of expression cassettes at defined chromosomal loci. Biochemistry 1994; 33:12746-12751. dx.doi.org/10.1021/bi00209a003
  10. Bode J, Kohwi Y, Dickinson L, Joh T, Klehr D, Mielke C, Kohwi-Shigematsu T (1992) Biological significance of unwinding capability of nuclear matrix-associating DNAs. Science 1992; 255, 195-197. dx.doi.org/10.1126/science.1553545


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